The VP1 unique region of human parvovirus B19 and human bocavirus induce lung injury in naïve Balb/c mice.
Both human parvovirus B19 (B19V) and human bocavirus (HBoV) are known to be important human pathogens of the Parvoviridae family. Our earlier investigation demonstrated that both B19V-VP1u and HBoV-VP1u have a significantly disruptive effect on tight junctions (TJs) in A549 cells, implying the essen...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6095614?pdf=render |
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| author | Chun-Yu Lin Yu-Han Chung Ya-Fang Shi Bor-Show Tzang Tsai-Ching Hsu |
| author_facet | Chun-Yu Lin Yu-Han Chung Ya-Fang Shi Bor-Show Tzang Tsai-Ching Hsu |
| author_sort | Chun-Yu Lin |
| collection | DOAJ |
| description | Both human parvovirus B19 (B19V) and human bocavirus (HBoV) are known to be important human pathogens of the Parvoviridae family. Our earlier investigation demonstrated that both B19V-VP1u and HBoV-VP1u have a significantly disruptive effect on tight junctions (TJs) in A549 cells, implying the essential role of parvovirus in airway infection and lung injury. However, no direct evidence that B19V-VP1u and HBoV-VP1u induce lung injury exists. The present study further investigates the induction of lung injury by B19V-VP1u and HBoV-VP1u in naïve Balb/c mice following subcutaneous injection of PBS, recombinant B19V-VP1u or HBoV-VP1u. The experimental results reveal significantly increased activity, protein expression and ratio of matrix metalloproteinase-9 (MMP-9) to MMP-2 in Balb/c mice that received B19V-VP1u or HBoV-VP1u compared to those that received PBS. Significantly higher levels of inflammatory cytokines, including IL-6 and IL-1β, and greater lymphocyte infiltration in lung tissue sections were detected in mice that received B19V-VP1u or HBoV-VP1u. Additionally, significantly increased levels of phosphorylated p65 (NF-κB) and MAPK signaling proteins were observed in lung tissue of mice that received B19V-VP1u or HBoV-VP1u compared to those of mice that received PBS. These findings demonstrate for the first time that B19V-VP1u and HBoV-VP1u proteins induce lung inflammatory reactions through p65 (NF-κB) and MAPK signaling. |
| first_indexed | 2024-12-10T18:28:19Z |
| format | Article |
| id | doaj.art-2511c0de9f0d46d28d38a050537044bb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T18:28:19Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2511c0de9f0d46d28d38a050537044bb2022-12-22T01:38:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01138e020266710.1371/journal.pone.0202667The VP1 unique region of human parvovirus B19 and human bocavirus induce lung injury in naïve Balb/c mice.Chun-Yu LinYu-Han ChungYa-Fang ShiBor-Show TzangTsai-Ching HsuBoth human parvovirus B19 (B19V) and human bocavirus (HBoV) are known to be important human pathogens of the Parvoviridae family. Our earlier investigation demonstrated that both B19V-VP1u and HBoV-VP1u have a significantly disruptive effect on tight junctions (TJs) in A549 cells, implying the essential role of parvovirus in airway infection and lung injury. However, no direct evidence that B19V-VP1u and HBoV-VP1u induce lung injury exists. The present study further investigates the induction of lung injury by B19V-VP1u and HBoV-VP1u in naïve Balb/c mice following subcutaneous injection of PBS, recombinant B19V-VP1u or HBoV-VP1u. The experimental results reveal significantly increased activity, protein expression and ratio of matrix metalloproteinase-9 (MMP-9) to MMP-2 in Balb/c mice that received B19V-VP1u or HBoV-VP1u compared to those that received PBS. Significantly higher levels of inflammatory cytokines, including IL-6 and IL-1β, and greater lymphocyte infiltration in lung tissue sections were detected in mice that received B19V-VP1u or HBoV-VP1u. Additionally, significantly increased levels of phosphorylated p65 (NF-κB) and MAPK signaling proteins were observed in lung tissue of mice that received B19V-VP1u or HBoV-VP1u compared to those of mice that received PBS. These findings demonstrate for the first time that B19V-VP1u and HBoV-VP1u proteins induce lung inflammatory reactions through p65 (NF-κB) and MAPK signaling.http://europepmc.org/articles/PMC6095614?pdf=render |
| spellingShingle | Chun-Yu Lin Yu-Han Chung Ya-Fang Shi Bor-Show Tzang Tsai-Ching Hsu The VP1 unique region of human parvovirus B19 and human bocavirus induce lung injury in naïve Balb/c mice. PLoS ONE |
| title | The VP1 unique region of human parvovirus B19 and human bocavirus induce lung injury in naïve Balb/c mice. |
| title_full | The VP1 unique region of human parvovirus B19 and human bocavirus induce lung injury in naïve Balb/c mice. |
| title_fullStr | The VP1 unique region of human parvovirus B19 and human bocavirus induce lung injury in naïve Balb/c mice. |
| title_full_unstemmed | The VP1 unique region of human parvovirus B19 and human bocavirus induce lung injury in naïve Balb/c mice. |
| title_short | The VP1 unique region of human parvovirus B19 and human bocavirus induce lung injury in naïve Balb/c mice. |
| title_sort | vp1 unique region of human parvovirus b19 and human bocavirus induce lung injury in naive balb c mice |
| url | http://europepmc.org/articles/PMC6095614?pdf=render |
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