Bot Gummosis of Lemon (<i>Citrus</i> × <i>limon</i>) Caused by <i>Neofusicoccum parvum</i>
<i>Neofusicoccum parvum</i>, in the family Botryosphaeriaceae, was identified as the causal agent of bot gummosis of lemon (<i>Citrus</i> × <i>limon</i>) trees, in the two major lemon-producing regions in Italy. Gummy cankers on trunk and scaffold branches of matu...
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MDPI AG
2021-04-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/4/294 |
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| author | Francesco Aloi Mario Riolo Rossana Parlascino Antonella Pane Santa Olga Cacciola |
| author_facet | Francesco Aloi Mario Riolo Rossana Parlascino Antonella Pane Santa Olga Cacciola |
| author_sort | Francesco Aloi |
| collection | DOAJ |
| description | <i>Neofusicoccum parvum</i>, in the family Botryosphaeriaceae, was identified as the causal agent of bot gummosis of lemon (<i>Citrus</i> × <i>limon</i>) trees, in the two major lemon-producing regions in Italy. Gummy cankers on trunk and scaffold branches of mature trees were the most typical disease symptoms. <i>Neofusicoccum parvum</i> was the sole fungus constantly and consistently isolated from the canker bark of symptomatic lemon trees. It was identified on the basis of morphological characters and the phylogenetic analysis of three loci, i.e., the internal transcribed spacer of nuclear ribosomal DNA (ITS) as well as the translation elongation factor 1-alpha (<i>TEF1</i>) and β-tubulin (<i>TUB2</i>) genes. The pathogenicity of <i>N. parvum</i> was demonstrated by wound inoculating two lemon cultivars, ‘Femminello 2kr’ and ‘Monachello’, as well as citrange (<i>C. sinensis</i> × <i>Poncirus trifoliata</i>) ‘Carrizo’ rootstock. In artificial inoculations, the fungus was very aggressive on lemons and weakly virulent on citrange, consistently with symptoms observed in the field as a consequence of natural infections. This is the first report of <i>N. parvum</i>, both in a wide and in a strict taxonomic sense, as a pathogen of lemon in Italy. |
| first_indexed | 2024-03-10T12:20:56Z |
| format | Article |
| id | doaj.art-25142fcc3ff24498963439c611cf95b3 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T12:20:56Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-25142fcc3ff24498963439c611cf95b32023-11-21T15:29:41ZengMDPI AGJournal of Fungi2309-608X2021-04-017429410.3390/jof7040294Bot Gummosis of Lemon (<i>Citrus</i> × <i>limon</i>) Caused by <i>Neofusicoccum parvum</i>Francesco Aloi0Mario Riolo1Rossana Parlascino2Antonella Pane3Santa Olga Cacciola4Department of Agriculture, Food and Environment, University of Catania, 95123 Catania, ItalyDepartment of Agriculture, Food and Environment, University of Catania, 95123 Catania, ItalyDepartment of Agriculture, Food and Environment, University of Catania, 95123 Catania, ItalyDepartment of Agriculture, Food and Environment, University of Catania, 95123 Catania, ItalyDepartment of Agriculture, Food and Environment, University of Catania, 95123 Catania, Italy<i>Neofusicoccum parvum</i>, in the family Botryosphaeriaceae, was identified as the causal agent of bot gummosis of lemon (<i>Citrus</i> × <i>limon</i>) trees, in the two major lemon-producing regions in Italy. Gummy cankers on trunk and scaffold branches of mature trees were the most typical disease symptoms. <i>Neofusicoccum parvum</i> was the sole fungus constantly and consistently isolated from the canker bark of symptomatic lemon trees. It was identified on the basis of morphological characters and the phylogenetic analysis of three loci, i.e., the internal transcribed spacer of nuclear ribosomal DNA (ITS) as well as the translation elongation factor 1-alpha (<i>TEF1</i>) and β-tubulin (<i>TUB2</i>) genes. The pathogenicity of <i>N. parvum</i> was demonstrated by wound inoculating two lemon cultivars, ‘Femminello 2kr’ and ‘Monachello’, as well as citrange (<i>C. sinensis</i> × <i>Poncirus trifoliata</i>) ‘Carrizo’ rootstock. In artificial inoculations, the fungus was very aggressive on lemons and weakly virulent on citrange, consistently with symptoms observed in the field as a consequence of natural infections. This is the first report of <i>N. parvum</i>, both in a wide and in a strict taxonomic sense, as a pathogen of lemon in Italy.https://www.mdpi.com/2309-608X/7/4/294Botryosphaeriaceae<i>ITS</i><i>TEF1</i><i>TUB2</i>citrustrunk and branch cankers |
| spellingShingle | Francesco Aloi Mario Riolo Rossana Parlascino Antonella Pane Santa Olga Cacciola Bot Gummosis of Lemon (<i>Citrus</i> × <i>limon</i>) Caused by <i>Neofusicoccum parvum</i> Journal of Fungi Botryosphaeriaceae <i>ITS</i> <i>TEF1</i> <i>TUB2</i> citrus trunk and branch cankers |
| title | Bot Gummosis of Lemon (<i>Citrus</i> × <i>limon</i>) Caused by <i>Neofusicoccum parvum</i> |
| title_full | Bot Gummosis of Lemon (<i>Citrus</i> × <i>limon</i>) Caused by <i>Neofusicoccum parvum</i> |
| title_fullStr | Bot Gummosis of Lemon (<i>Citrus</i> × <i>limon</i>) Caused by <i>Neofusicoccum parvum</i> |
| title_full_unstemmed | Bot Gummosis of Lemon (<i>Citrus</i> × <i>limon</i>) Caused by <i>Neofusicoccum parvum</i> |
| title_short | Bot Gummosis of Lemon (<i>Citrus</i> × <i>limon</i>) Caused by <i>Neofusicoccum parvum</i> |
| title_sort | bot gummosis of lemon i citrus i i limon i caused by i neofusicoccum parvum i |
| topic | Botryosphaeriaceae <i>ITS</i> <i>TEF1</i> <i>TUB2</i> citrus trunk and branch cankers |
| url | https://www.mdpi.com/2309-608X/7/4/294 |
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