First waikavirus infectious clones and vascular expression of green fluorescent protein from maize chlorotic dwarf virus
Plant viruses classified in the genus Waikavirus, family Secoviridae, are positive sense single-stranded RNA viruses that include important pathogens of maize (maize chlorotic dwarf virus; MCDV) and rice (rice tungro spherical virus; RTSV). Many aspects of the molecular biology of waikaviruses remai...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Virology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fviro.2023.1216285/full |
| _version_ | 1797791860625768448 |
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| author | Lucy R. Stewart Kristen Willie Wenshuang Xie Jane Todd Hong Hanh Tran |
| author_facet | Lucy R. Stewart Kristen Willie Wenshuang Xie Jane Todd Hong Hanh Tran |
| author_sort | Lucy R. Stewart |
| collection | DOAJ |
| description | Plant viruses classified in the genus Waikavirus, family Secoviridae, are positive sense single-stranded RNA viruses that include important pathogens of maize (maize chlorotic dwarf virus; MCDV) and rice (rice tungro spherical virus; RTSV). Many aspects of the molecular biology of waikaviruses remain unexplored because of experimental challenges and lack of infectious clones for low titer, phloem-limited, and obligately vector-transmitted waikaviruses. Here we report the first development of waikavirus infectious clones for two MCDV strains, MCDV-S and MCDV-M1, and insect-free launching of infections from these clones in maize by vascular puncture inoculation. We further developed a green fluorescent protein (GFP)-tagged MCDV clone by replacing the viral p27-encoding sequence with GFP-encoding sequence. GFP-tagged virus moved systemically in plants and caused symptomatic infection similar to wild type virus, with vascular expression of GFP. Development of waikavirus infectious clones is a major advance for this group of agriculturally significant viruses. |
| first_indexed | 2024-03-13T02:24:49Z |
| format | Article |
| id | doaj.art-2516c75ddc5146389a8e9c48dae682c1 |
| institution | Directory Open Access Journal |
| issn | 2673-818X |
| language | English |
| last_indexed | 2024-03-13T02:24:49Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Virology |
| spelling | doaj.art-2516c75ddc5146389a8e9c48dae682c12023-06-30T04:37:19ZengFrontiers Media S.A.Frontiers in Virology2673-818X2023-06-01310.3389/fviro.2023.12162851216285First waikavirus infectious clones and vascular expression of green fluorescent protein from maize chlorotic dwarf virusLucy R. Stewart0Kristen Willie1Wenshuang Xie2Jane Todd3Hong Hanh Tran4Foreign Disease-Weed Science Research Unit, United States Department of Agriculture, Agricultural Research Service (USDA, ARS), Frederick, MD, United StatesCorn, Soybean and Wheat Quality Research Unit, United States Department of Agriculture, Agricultural Research Service (USDA, ARS), Wooster, OH, United StatesDepartment of Plant Pathology, The Ohio State University, Wooster, OH, United StatesCorn, Soybean and Wheat Quality Research Unit, United States Department of Agriculture, Agricultural Research Service (USDA, ARS), Wooster, OH, United StatesDepartment of Plant Pathology, The Ohio State University, Wooster, OH, United StatesPlant viruses classified in the genus Waikavirus, family Secoviridae, are positive sense single-stranded RNA viruses that include important pathogens of maize (maize chlorotic dwarf virus; MCDV) and rice (rice tungro spherical virus; RTSV). Many aspects of the molecular biology of waikaviruses remain unexplored because of experimental challenges and lack of infectious clones for low titer, phloem-limited, and obligately vector-transmitted waikaviruses. Here we report the first development of waikavirus infectious clones for two MCDV strains, MCDV-S and MCDV-M1, and insect-free launching of infections from these clones in maize by vascular puncture inoculation. We further developed a green fluorescent protein (GFP)-tagged MCDV clone by replacing the viral p27-encoding sequence with GFP-encoding sequence. GFP-tagged virus moved systemically in plants and caused symptomatic infection similar to wild type virus, with vascular expression of GFP. Development of waikavirus infectious clones is a major advance for this group of agriculturally significant viruses.https://www.frontiersin.org/articles/10.3389/fviro.2023.1216285/fullWaikavirusSecoviridaeinfectious clonevirus vectormaizeleafhopper transmission |
| spellingShingle | Lucy R. Stewart Kristen Willie Wenshuang Xie Jane Todd Hong Hanh Tran First waikavirus infectious clones and vascular expression of green fluorescent protein from maize chlorotic dwarf virus Frontiers in Virology Waikavirus Secoviridae infectious clone virus vector maize leafhopper transmission |
| title | First waikavirus infectious clones and vascular expression of green fluorescent protein from maize chlorotic dwarf virus |
| title_full | First waikavirus infectious clones and vascular expression of green fluorescent protein from maize chlorotic dwarf virus |
| title_fullStr | First waikavirus infectious clones and vascular expression of green fluorescent protein from maize chlorotic dwarf virus |
| title_full_unstemmed | First waikavirus infectious clones and vascular expression of green fluorescent protein from maize chlorotic dwarf virus |
| title_short | First waikavirus infectious clones and vascular expression of green fluorescent protein from maize chlorotic dwarf virus |
| title_sort | first waikavirus infectious clones and vascular expression of green fluorescent protein from maize chlorotic dwarf virus |
| topic | Waikavirus Secoviridae infectious clone virus vector maize leafhopper transmission |
| url | https://www.frontiersin.org/articles/10.3389/fviro.2023.1216285/full |
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