Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco
Abstract Background Bovine tuberculosis (BTB) is caused by Mycobacterium bovis, which belongs to the Mycobacterium tuberculosis complex. Mycobacterium bovis have been described to be responsible of most cases of bovine tuberculosis. Although M. tuberculosis, M. africanum and non-complex mycobacteria...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-08-01
|
| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-017-1165-6 |
| _version_ | 1818952397648560128 |
|---|---|
| author | Hind Yahyaoui-Azami Hamid Aboukhassib Mohammed Bouslikhane Jaouad Berrada Soukaina Rami Miriam Reinhard Sebastien Gagneux Julia Feldmann Sonia Borrell Jakob Zinsstag |
| author_facet | Hind Yahyaoui-Azami Hamid Aboukhassib Mohammed Bouslikhane Jaouad Berrada Soukaina Rami Miriam Reinhard Sebastien Gagneux Julia Feldmann Sonia Borrell Jakob Zinsstag |
| author_sort | Hind Yahyaoui-Azami |
| collection | DOAJ |
| description | Abstract Background Bovine tuberculosis (BTB) is caused by Mycobacterium bovis, which belongs to the Mycobacterium tuberculosis complex. Mycobacterium bovis have been described to be responsible of most cases of bovine tuberculosis. Although M. tuberculosis, M. africanum and non-complex mycobacteria were isolated from cattle. In Morocco, so far, no molecular studies were conducted to characterize the strains responsible of BTB. The present study aims to characterize M. bovis in Morocco. The present study was conducted in slaughterhouses in Rabat and El Jadida. Samples were collected from 327 slaughtered animals with visible lesions suggesting BTB. Results A total of 225 isolates yielded cultures, 95% (n = 215) of them were acid-fast (AF). Sixty eight per cent of the AF positive samples were confirmed as tuberculous mycobacteria (n = 147), 99% of these (n = 146) having RD9 and among the latter, 98% (n = 143) positive while 2% (n = 3) negative for RD4 A total of 134 samples were analyzed by spoligotyping of which 14 were in cluster and with 41 different spoligotypes, ten of them were new patterns (23%). The most prevalent spoligotypes were SB0121, SB0265, and SB0120, and were already identified in many other countries, such as Algeria, Spain, Tunisia, the United States and Argentina. Conclusion The shared borders between Algeria and Morocco, in addition to the previous importation of cattle from Europe and the US could explain the similarities found in M. bovis spoligotypes. On the other hand, the desert of Morocco could be considered as an efficient barrier preventing the introduction of BTB to Morocco from West Central and East Africa. Our findings suggest a low level endemic transmission of BTB similar to other African countries. However, more research is needed for further knowledge about the transmission patterns of BTB in Morocco. |
| first_indexed | 2024-12-20T09:49:44Z |
| format | Article |
| id | doaj.art-251956c5edfa4cbf9f74e61dfbcb79d7 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-20T09:49:44Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-251956c5edfa4cbf9f74e61dfbcb79d72022-12-21T19:44:37ZengBMCBMC Veterinary Research1746-61482017-08-011311710.1186/s12917-017-1165-6Molecular characterization of bovine tuberculosis strains in two slaughterhouses in MoroccoHind Yahyaoui-Azami0Hamid Aboukhassib1Mohammed Bouslikhane2Jaouad Berrada3Soukaina Rami4Miriam Reinhard5Sebastien Gagneux6Julia Feldmann7Sonia Borrell8Jakob Zinsstag9Department of Epidemiology and Public Health, Swiss Tropical and Public Health InstituteOffice Nationale de Sécurité Sanitaire des produits AlimentairesDepartment of Pathology and Veterinary Public Health, Hassan II Agronomy and Veterinary InstituteDepartment of Pathology and Veterinary Public Health, Hassan II Agronomy and Veterinary InstituteDepartment of Pathology and Veterinary Public Health, Hassan II Agronomy and Veterinary InstituteUniversity of BaselUniversity of BaselUniversity of BaselUniversity of BaselDepartment of Epidemiology and Public Health, Swiss Tropical and Public Health InstituteAbstract Background Bovine tuberculosis (BTB) is caused by Mycobacterium bovis, which belongs to the Mycobacterium tuberculosis complex. Mycobacterium bovis have been described to be responsible of most cases of bovine tuberculosis. Although M. tuberculosis, M. africanum and non-complex mycobacteria were isolated from cattle. In Morocco, so far, no molecular studies were conducted to characterize the strains responsible of BTB. The present study aims to characterize M. bovis in Morocco. The present study was conducted in slaughterhouses in Rabat and El Jadida. Samples were collected from 327 slaughtered animals with visible lesions suggesting BTB. Results A total of 225 isolates yielded cultures, 95% (n = 215) of them were acid-fast (AF). Sixty eight per cent of the AF positive samples were confirmed as tuberculous mycobacteria (n = 147), 99% of these (n = 146) having RD9 and among the latter, 98% (n = 143) positive while 2% (n = 3) negative for RD4 A total of 134 samples were analyzed by spoligotyping of which 14 were in cluster and with 41 different spoligotypes, ten of them were new patterns (23%). The most prevalent spoligotypes were SB0121, SB0265, and SB0120, and were already identified in many other countries, such as Algeria, Spain, Tunisia, the United States and Argentina. Conclusion The shared borders between Algeria and Morocco, in addition to the previous importation of cattle from Europe and the US could explain the similarities found in M. bovis spoligotypes. On the other hand, the desert of Morocco could be considered as an efficient barrier preventing the introduction of BTB to Morocco from West Central and East Africa. Our findings suggest a low level endemic transmission of BTB similar to other African countries. However, more research is needed for further knowledge about the transmission patterns of BTB in Morocco.http://link.springer.com/article/10.1186/s12917-017-1165-6Mycobacterium bovisBovine tuberculosisMoroccoCattleSlaughterhouseSpoligotype |
| spellingShingle | Hind Yahyaoui-Azami Hamid Aboukhassib Mohammed Bouslikhane Jaouad Berrada Soukaina Rami Miriam Reinhard Sebastien Gagneux Julia Feldmann Sonia Borrell Jakob Zinsstag Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco BMC Veterinary Research Mycobacterium bovis Bovine tuberculosis Morocco Cattle Slaughterhouse Spoligotype |
| title | Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco |
| title_full | Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco |
| title_fullStr | Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco |
| title_full_unstemmed | Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco |
| title_short | Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco |
| title_sort | molecular characterization of bovine tuberculosis strains in two slaughterhouses in morocco |
| topic | Mycobacterium bovis Bovine tuberculosis Morocco Cattle Slaughterhouse Spoligotype |
| url | http://link.springer.com/article/10.1186/s12917-017-1165-6 |
| work_keys_str_mv | AT hindyahyaouiazami molecularcharacterizationofbovinetuberculosisstrainsintwoslaughterhousesinmorocco AT hamidaboukhassib molecularcharacterizationofbovinetuberculosisstrainsintwoslaughterhousesinmorocco AT mohammedbouslikhane molecularcharacterizationofbovinetuberculosisstrainsintwoslaughterhousesinmorocco AT jaouadberrada molecularcharacterizationofbovinetuberculosisstrainsintwoslaughterhousesinmorocco AT soukainarami molecularcharacterizationofbovinetuberculosisstrainsintwoslaughterhousesinmorocco AT miriamreinhard molecularcharacterizationofbovinetuberculosisstrainsintwoslaughterhousesinmorocco AT sebastiengagneux molecularcharacterizationofbovinetuberculosisstrainsintwoslaughterhousesinmorocco AT juliafeldmann molecularcharacterizationofbovinetuberculosisstrainsintwoslaughterhousesinmorocco AT soniaborrell molecularcharacterizationofbovinetuberculosisstrainsintwoslaughterhousesinmorocco AT jakobzinsstag molecularcharacterizationofbovinetuberculosisstrainsintwoslaughterhousesinmorocco |