The Role of <i>TSHR, PTEN</i> and <i>RASSF1A</i> Promoters’ Methylation Status for Non-Invasive Detection of Papillary Thyroid Carcinoma
Aim: We investigated whether a difference exists between <i>TSHR, PTEN</i> and <i>RASSF1A</i> methylation status in plasma of subjects with papillary thyroid cancer (PTC). Methods: Peripheral blood samples were collected from 68 patients with PTC and 86 healthy controls (HC)....
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/16/4917 |
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| author | Raimonda Klimaitė Mintautė Kazokaitė Aistė Kondrotienė Dalia Daukšienė Rasa Sabaliauskaitė Kristina Žukauskaitė Birutė Žilaitienė Sonata Jarmalaitė Albertas Daukša |
| author_facet | Raimonda Klimaitė Mintautė Kazokaitė Aistė Kondrotienė Dalia Daukšienė Rasa Sabaliauskaitė Kristina Žukauskaitė Birutė Žilaitienė Sonata Jarmalaitė Albertas Daukša |
| author_sort | Raimonda Klimaitė |
| collection | DOAJ |
| description | Aim: We investigated whether a difference exists between <i>TSHR, PTEN</i> and <i>RASSF1A</i> methylation status in plasma of subjects with papillary thyroid cancer (PTC). Methods: Peripheral blood samples were collected from 68 patients with PTC and 86 healthy controls (HC). Thyroid cancer tissue and corresponding adjacent normal tissue methylation levels were analyzed. DNA methylation level changes in <i>TSHR, PTEN</i> and <i>RASSF1A</i> genes were analyzed by quantitative methylation-sensitive polymerase chain reaction. Results: We observed that the methylation level of <i>TSHR</i> was significantly higher in the thyroid cancer tissue compared to adjacent normal tissue (<i>p</i> = 0.040). <i>TSHR</i> methylation levels in the PTC group plasma samples were significantly higher compared to HC (<i>p</i> = 0.022). After surgery, PTC plasma samples showed lower <i>TSHR</i> and <i>PTEN</i> methylation levels compared to the levels before surgery (<i>p</i> = 0.003, <i>p</i> = 0.031, respectively). The <i>TSHR</i> methylation level was significantly higher in PTC with larger tumor size (>2 cm) (<i>p</i> < 0.001), and lymph node metastases (<i>p</i> = 0.01), lymphovascular invasion (<i>p</i> = 0.02) and multifocality (<i>p</i> = 0.013) 0ROC analysis revealed that the <i>TSHR</i> methylation level provides high accuracy in distinguishing PTC from HC (<i>p</i> = 0.022, AUC of 0.616). Conclusion: <i>TSHR</i> methylation in peripheral blood samples is expected to be a sensitive and specific minimally invasive tool for the diagnosis of PTC, especially in combination with other diagnostic means. |
| first_indexed | 2024-03-09T04:16:15Z |
| format | Article |
| id | doaj.art-251af1c0a58542ad8286fe28b8cdd884 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T04:16:15Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-251af1c0a58542ad8286fe28b8cdd8842023-12-03T13:53:24ZengMDPI AGJournal of Clinical Medicine2077-03832022-08-011116491710.3390/jcm11164917The Role of <i>TSHR, PTEN</i> and <i>RASSF1A</i> Promoters’ Methylation Status for Non-Invasive Detection of Papillary Thyroid CarcinomaRaimonda Klimaitė0Mintautė Kazokaitė1Aistė Kondrotienė2Dalia Daukšienė3Rasa Sabaliauskaitė4Kristina Žukauskaitė5Birutė Žilaitienė6Sonata Jarmalaitė7Albertas Daukša8Institute of Endocrinology, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, LithuaniaInstitute of Endocrinology, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, LithuaniaInstitute of Endocrinology, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, LithuaniaInstitute of Endocrinology, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, LithuaniaLaboratory of Genetic Diagnostic, National Cancer Institute, 50009 Vilnius, LithuaniaLaboratory of Genetic Diagnostic, National Cancer Institute, 50009 Vilnius, LithuaniaInstitute of Endocrinology, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, LithuaniaLaboratory of Genetic Diagnostic, National Cancer Institute, 50009 Vilnius, LithuaniaInstitute of Digestive Research, Faculty of Medicine, Medical Academy, Lithuanian University of Health Sciences, 50161 Kaunas, LithuaniaAim: We investigated whether a difference exists between <i>TSHR, PTEN</i> and <i>RASSF1A</i> methylation status in plasma of subjects with papillary thyroid cancer (PTC). Methods: Peripheral blood samples were collected from 68 patients with PTC and 86 healthy controls (HC). Thyroid cancer tissue and corresponding adjacent normal tissue methylation levels were analyzed. DNA methylation level changes in <i>TSHR, PTEN</i> and <i>RASSF1A</i> genes were analyzed by quantitative methylation-sensitive polymerase chain reaction. Results: We observed that the methylation level of <i>TSHR</i> was significantly higher in the thyroid cancer tissue compared to adjacent normal tissue (<i>p</i> = 0.040). <i>TSHR</i> methylation levels in the PTC group plasma samples were significantly higher compared to HC (<i>p</i> = 0.022). After surgery, PTC plasma samples showed lower <i>TSHR</i> and <i>PTEN</i> methylation levels compared to the levels before surgery (<i>p</i> = 0.003, <i>p</i> = 0.031, respectively). The <i>TSHR</i> methylation level was significantly higher in PTC with larger tumor size (>2 cm) (<i>p</i> < 0.001), and lymph node metastases (<i>p</i> = 0.01), lymphovascular invasion (<i>p</i> = 0.02) and multifocality (<i>p</i> = 0.013) 0ROC analysis revealed that the <i>TSHR</i> methylation level provides high accuracy in distinguishing PTC from HC (<i>p</i> = 0.022, AUC of 0.616). Conclusion: <i>TSHR</i> methylation in peripheral blood samples is expected to be a sensitive and specific minimally invasive tool for the diagnosis of PTC, especially in combination with other diagnostic means.https://www.mdpi.com/2077-0383/11/16/4917papillary thyroid carcinomaPTCmethylation<i>TSHR</i><i>PTEN</i><i>RASSF1A</i> |
| spellingShingle | Raimonda Klimaitė Mintautė Kazokaitė Aistė Kondrotienė Dalia Daukšienė Rasa Sabaliauskaitė Kristina Žukauskaitė Birutė Žilaitienė Sonata Jarmalaitė Albertas Daukša The Role of <i>TSHR, PTEN</i> and <i>RASSF1A</i> Promoters’ Methylation Status for Non-Invasive Detection of Papillary Thyroid Carcinoma Journal of Clinical Medicine papillary thyroid carcinoma PTC methylation <i>TSHR</i> <i>PTEN</i> <i>RASSF1A</i> |
| title | The Role of <i>TSHR, PTEN</i> and <i>RASSF1A</i> Promoters’ Methylation Status for Non-Invasive Detection of Papillary Thyroid Carcinoma |
| title_full | The Role of <i>TSHR, PTEN</i> and <i>RASSF1A</i> Promoters’ Methylation Status for Non-Invasive Detection of Papillary Thyroid Carcinoma |
| title_fullStr | The Role of <i>TSHR, PTEN</i> and <i>RASSF1A</i> Promoters’ Methylation Status for Non-Invasive Detection of Papillary Thyroid Carcinoma |
| title_full_unstemmed | The Role of <i>TSHR, PTEN</i> and <i>RASSF1A</i> Promoters’ Methylation Status for Non-Invasive Detection of Papillary Thyroid Carcinoma |
| title_short | The Role of <i>TSHR, PTEN</i> and <i>RASSF1A</i> Promoters’ Methylation Status for Non-Invasive Detection of Papillary Thyroid Carcinoma |
| title_sort | role of i tshr pten i and i rassf1a i promoters methylation status for non invasive detection of papillary thyroid carcinoma |
| topic | papillary thyroid carcinoma PTC methylation <i>TSHR</i> <i>PTEN</i> <i>RASSF1A</i> |
| url | https://www.mdpi.com/2077-0383/11/16/4917 |
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