Effect of α-linolenic acid on endoplasmic reticulum stress-mediated apoptosis of palmitic acid lipotoxicity in primary rat hepatocytes
<p>Abstract</p> <p>Background</p> <p>Hepatic inflammation and degeneration induced by lipid depositions may be the major cause of nonalcoholic fatty liver disease (NAFLD). In this study, we investigated the effects of saturated and unsaturated fatty acids (FA) on apopto...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-07-01
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| Series: | Lipids in Health and Disease |
| Online Access: | http://www.lipidworld.com/content/10/1/122 |
| _version_ | 1811294358772645888 |
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| author | Dong Lei Shi Hongyang Yang Xia Zhang Yong Bai Jian |
| author_facet | Dong Lei Shi Hongyang Yang Xia Zhang Yong Bai Jian |
| author_sort | Dong Lei |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Hepatic inflammation and degeneration induced by lipid depositions may be the major cause of nonalcoholic fatty liver disease (NAFLD). In this study, we investigated the effects of saturated and unsaturated fatty acids (FA) on apoptosis in primary rat hepatocytes.</p> <p>Methods</p> <p>The primary rat hepatocytes were treated with palmitic acid and/or α-linolenic acid in vitro. The expression of proteins associated with endoplasmic reticulum (ER) stress, apoptosis, caspase-3 levels were detected after the treatment.</p> <p>Results</p> <p>The treatment with palmitic acid produced a significant increase in cell death. The unfolded protein response (UPR)-associated genes CHOP, GRP78, and GRP94 were induced to higher expression levels by palmitic acid. Co-treatment with α-linolenic acid reversed the apoptotic effect and levels of all three indicators of ER stress exerted by palmitic acid. Tunicamycin, which induces ER stress produced similar effects to those obtained using palmitic acid; its effects were also reversed by α-linolenic acid.</p> <p>Conclusions</p> <p>α-Linolenic acid may provide a useful strategy to avoid the lipotoxicity of dietary palmitic acid and nutrient overload accompanied with obesity and NAFLD.</p> |
| first_indexed | 2024-04-13T05:16:14Z |
| format | Article |
| id | doaj.art-251b575abbfc48179758685f40ba834a |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-04-13T05:16:14Z |
| publishDate | 2011-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-251b575abbfc48179758685f40ba834a2022-12-22T03:00:53ZengBMCLipids in Health and Disease1476-511X2011-07-0110112210.1186/1476-511X-10-122Effect of α-linolenic acid on endoplasmic reticulum stress-mediated apoptosis of palmitic acid lipotoxicity in primary rat hepatocytesDong LeiShi HongyangYang XiaZhang YongBai Jian<p>Abstract</p> <p>Background</p> <p>Hepatic inflammation and degeneration induced by lipid depositions may be the major cause of nonalcoholic fatty liver disease (NAFLD). In this study, we investigated the effects of saturated and unsaturated fatty acids (FA) on apoptosis in primary rat hepatocytes.</p> <p>Methods</p> <p>The primary rat hepatocytes were treated with palmitic acid and/or α-linolenic acid in vitro. The expression of proteins associated with endoplasmic reticulum (ER) stress, apoptosis, caspase-3 levels were detected after the treatment.</p> <p>Results</p> <p>The treatment with palmitic acid produced a significant increase in cell death. The unfolded protein response (UPR)-associated genes CHOP, GRP78, and GRP94 were induced to higher expression levels by palmitic acid. Co-treatment with α-linolenic acid reversed the apoptotic effect and levels of all three indicators of ER stress exerted by palmitic acid. Tunicamycin, which induces ER stress produced similar effects to those obtained using palmitic acid; its effects were also reversed by α-linolenic acid.</p> <p>Conclusions</p> <p>α-Linolenic acid may provide a useful strategy to avoid the lipotoxicity of dietary palmitic acid and nutrient overload accompanied with obesity and NAFLD.</p>http://www.lipidworld.com/content/10/1/122 |
| spellingShingle | Dong Lei Shi Hongyang Yang Xia Zhang Yong Bai Jian Effect of α-linolenic acid on endoplasmic reticulum stress-mediated apoptosis of palmitic acid lipotoxicity in primary rat hepatocytes Lipids in Health and Disease |
| title | Effect of α-linolenic acid on endoplasmic reticulum stress-mediated apoptosis of palmitic acid lipotoxicity in primary rat hepatocytes |
| title_full | Effect of α-linolenic acid on endoplasmic reticulum stress-mediated apoptosis of palmitic acid lipotoxicity in primary rat hepatocytes |
| title_fullStr | Effect of α-linolenic acid on endoplasmic reticulum stress-mediated apoptosis of palmitic acid lipotoxicity in primary rat hepatocytes |
| title_full_unstemmed | Effect of α-linolenic acid on endoplasmic reticulum stress-mediated apoptosis of palmitic acid lipotoxicity in primary rat hepatocytes |
| title_short | Effect of α-linolenic acid on endoplasmic reticulum stress-mediated apoptosis of palmitic acid lipotoxicity in primary rat hepatocytes |
| title_sort | effect of α linolenic acid on endoplasmic reticulum stress mediated apoptosis of palmitic acid lipotoxicity in primary rat hepatocytes |
| url | http://www.lipidworld.com/content/10/1/122 |
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