Several Isoforms for Each Subunit Shared by RNA Polymerases are Differentially Expressed in the Cultivated Olive Tree (Olea europaea L.)
Plants contain five nuclear RNA polymerases, with RNA pols IV and V in addition to conserved eukaryotic RNA pols I, II, and III. These transcriptional complexes share five common subunits, which have been extensively analyzed only in yeasts. By taking advantage of the recently published olive tree c...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2021.679292/full |
| _version_ | 1819178020284399616 |
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| author | Isabel Fernández-Parras Jorge Antolín Ramírez-Tejero Francisco Luque Francisco Luque Francisco Navarro Francisco Navarro |
| author_facet | Isabel Fernández-Parras Jorge Antolín Ramírez-Tejero Francisco Luque Francisco Luque Francisco Navarro Francisco Navarro |
| author_sort | Isabel Fernández-Parras |
| collection | DOAJ |
| description | Plants contain five nuclear RNA polymerases, with RNA pols IV and V in addition to conserved eukaryotic RNA pols I, II, and III. These transcriptional complexes share five common subunits, which have been extensively analyzed only in yeasts. By taking advantage of the recently published olive tree cultivar (Olea europaea L. cv. Picual) genome, we performed a genome-wide analysis of the genomic composition corresponding to subunits common to RNA pols. The cultivated olive tree genome is quite complex and contains many genes with several copies. We also investigated, for the first time, gene expression patterns for subunits common to RNA pols using RNA-Seq under different economically and biologically relevant conditions for the cultivar “Picual”: tissues/organs, biotic and abiotic stresses, and early development from seeds. Our results demonstrated the existence of a multigene family of subunits common to RNA pols, and a variable number of paralogs for each subunit in the olive cultivar “Picual.” Furthermore, these isoforms display specific and differentiated expression profiles depending on the isoform and growth conditions, which may be relevant for their role in olive tree biology. |
| first_indexed | 2024-12-22T21:35:54Z |
| format | Article |
| id | doaj.art-251c70ff905341a3ac145c7eb7644f0f |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-22T21:35:54Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-251c70ff905341a3ac145c7eb7644f0f2022-12-21T18:11:46ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2021-12-01810.3389/fmolb.2021.679292679292Several Isoforms for Each Subunit Shared by RNA Polymerases are Differentially Expressed in the Cultivated Olive Tree (Olea europaea L.)Isabel Fernández-Parras0Jorge Antolín Ramírez-Tejero1Francisco Luque2Francisco Luque3Francisco Navarro4Francisco Navarro5Departamento de Biología Experimental-Genética, Jaén, SpainDepartamento de Biología Experimental-Genética, Jaén, SpainDepartamento de Biología Experimental-Genética, Jaén, SpainCentro de Estudios Avanzados en Aceite de Oliva y Olivar, Universidad de Jaén, Jaén, SpainDepartamento de Biología Experimental-Genética, Jaén, SpainCentro de Estudios Avanzados en Aceite de Oliva y Olivar, Universidad de Jaén, Jaén, SpainPlants contain five nuclear RNA polymerases, with RNA pols IV and V in addition to conserved eukaryotic RNA pols I, II, and III. These transcriptional complexes share five common subunits, which have been extensively analyzed only in yeasts. By taking advantage of the recently published olive tree cultivar (Olea europaea L. cv. Picual) genome, we performed a genome-wide analysis of the genomic composition corresponding to subunits common to RNA pols. The cultivated olive tree genome is quite complex and contains many genes with several copies. We also investigated, for the first time, gene expression patterns for subunits common to RNA pols using RNA-Seq under different economically and biologically relevant conditions for the cultivar “Picual”: tissues/organs, biotic and abiotic stresses, and early development from seeds. Our results demonstrated the existence of a multigene family of subunits common to RNA pols, and a variable number of paralogs for each subunit in the olive cultivar “Picual.” Furthermore, these isoforms display specific and differentiated expression profiles depending on the isoform and growth conditions, which may be relevant for their role in olive tree biology.https://www.frontiersin.org/articles/10.3389/fmolb.2021.679292/fullRNA polymerasesplantsoliveexpression conditionscommon subunits |
| spellingShingle | Isabel Fernández-Parras Jorge Antolín Ramírez-Tejero Francisco Luque Francisco Luque Francisco Navarro Francisco Navarro Several Isoforms for Each Subunit Shared by RNA Polymerases are Differentially Expressed in the Cultivated Olive Tree (Olea europaea L.) Frontiers in Molecular Biosciences RNA polymerases plants olive expression conditions common subunits |
| title | Several Isoforms for Each Subunit Shared by RNA Polymerases are Differentially Expressed in the Cultivated Olive Tree (Olea europaea L.) |
| title_full | Several Isoforms for Each Subunit Shared by RNA Polymerases are Differentially Expressed in the Cultivated Olive Tree (Olea europaea L.) |
| title_fullStr | Several Isoforms for Each Subunit Shared by RNA Polymerases are Differentially Expressed in the Cultivated Olive Tree (Olea europaea L.) |
| title_full_unstemmed | Several Isoforms for Each Subunit Shared by RNA Polymerases are Differentially Expressed in the Cultivated Olive Tree (Olea europaea L.) |
| title_short | Several Isoforms for Each Subunit Shared by RNA Polymerases are Differentially Expressed in the Cultivated Olive Tree (Olea europaea L.) |
| title_sort | several isoforms for each subunit shared by rna polymerases are differentially expressed in the cultivated olive tree olea europaea l |
| topic | RNA polymerases plants olive expression conditions common subunits |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2021.679292/full |
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