Folic acid and zinc improve hyperuricemia by altering the gut microbiota of rats with high-purine diet-induced hyperuricemia
A high-purine diet can cause hyperuricemia and destroy the microbial composition of the gut microbiota. Both folic acid and zinc significantly reduce uric acid levels and alleviate hyperuricemia. However, whether the underlying mechanisms are associated with the regulation of the gut microbiota rema...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.907952/full |
| _version_ | 1828777814495592448 |
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| author | Xuewei Sun Xuewei Sun Jie Wen Jie Wen Baosheng Guan Baosheng Guan Jialin Li Jialin Li Jincheng Luo Jincheng Luo Jie Li Jie Li Mingyu Wei Mingyu Wei Hongbin Qiu Hongbin Qiu |
| author_facet | Xuewei Sun Xuewei Sun Jie Wen Jie Wen Baosheng Guan Baosheng Guan Jialin Li Jialin Li Jincheng Luo Jincheng Luo Jie Li Jie Li Mingyu Wei Mingyu Wei Hongbin Qiu Hongbin Qiu |
| author_sort | Xuewei Sun |
| collection | DOAJ |
| description | A high-purine diet can cause hyperuricemia and destroy the microbial composition of the gut microbiota. Both folic acid and zinc significantly reduce uric acid levels and alleviate hyperuricemia. However, whether the underlying mechanisms are associated with the regulation of the gut microbiota remain unknown. To explore alterations of the gut microbiota related to folic acid and zinc treatment in rats with hyperuricemia in our study. A hyperuricemic rat model was established with a high-purine diet. The effects of folic acid and zinc on uric acid levels were evaluated. Alterations of the gut microbiota related to hyperuricemia and the treatments were evaluated by sequencing using the Illumina MiSeq system. The results demonstrated that uric acid levels dropped observably, and the activities of adenosine deaminase (ADA) and xanthine oxidase (XOD) were downregulated after folic acid or zinc intervention. 16S rRNA gene sequencing-based gut microbiota analysis revealed that folic acid and zinc enhanced the abundance of probiotic bacteria and reduced that of pathogenic bacteria, thus improving intestinal barrier function. PICRUST analysis indicated that folic acid and zinc restored gut microbiota metabolism. These findings indicate that folic acid and zinc ameliorate hyperuricemia by inhibiting uric acid biosynthesis and stimulating uric acid excretion by modulating the gut microbiota. Thus, folic acid and zinc may be new and safe therapeutic agents to improve hyperuricemia. |
| first_indexed | 2024-12-11T16:29:44Z |
| format | Article |
| id | doaj.art-251ca47229f1494e9f6130f491476202 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T16:29:44Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-251ca47229f1494e9f6130f4914762022022-12-22T00:58:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-07-011310.3389/fmicb.2022.907952907952Folic acid and zinc improve hyperuricemia by altering the gut microbiota of rats with high-purine diet-induced hyperuricemiaXuewei Sun0Xuewei Sun1Jie Wen2Jie Wen3Baosheng Guan4Baosheng Guan5Jialin Li6Jialin Li7Jincheng Luo8Jincheng Luo9Jie Li10Jie Li11Mingyu Wei12Mingyu Wei13Hongbin Qiu14Hongbin Qiu15School of Public Health, Jiamusi University, Jiamusi, ChinaHeilongjiang Provincial Key Laboratory of Gout Research, Jiamusi, ChinaSchool of Public Health, Jiamusi University, Jiamusi, ChinaHeilongjiang Provincial Key Laboratory of Gout Research, Jiamusi, ChinaSchool of Public Health, Jiamusi University, Jiamusi, ChinaHeilongjiang Provincial Key Laboratory of Gout Research, Jiamusi, ChinaSchool of Public Health, Jiamusi University, Jiamusi, ChinaHeilongjiang Provincial Key Laboratory of Gout Research, Jiamusi, ChinaSchool of Public Health, Jiamusi University, Jiamusi, ChinaHeilongjiang Provincial Key Laboratory of Gout Research, Jiamusi, ChinaSchool of Public Health, Jiamusi University, Jiamusi, ChinaHeilongjiang Provincial Key Laboratory of Gout Research, Jiamusi, ChinaSchool of Public Health, Jiamusi University, Jiamusi, ChinaHeilongjiang Provincial Key Laboratory of Gout Research, Jiamusi, ChinaSchool of Public Health, Jiamusi University, Jiamusi, ChinaHeilongjiang Provincial Key Laboratory of Gout Research, Jiamusi, ChinaA high-purine diet can cause hyperuricemia and destroy the microbial composition of the gut microbiota. Both folic acid and zinc significantly reduce uric acid levels and alleviate hyperuricemia. However, whether the underlying mechanisms are associated with the regulation of the gut microbiota remain unknown. To explore alterations of the gut microbiota related to folic acid and zinc treatment in rats with hyperuricemia in our study. A hyperuricemic rat model was established with a high-purine diet. The effects of folic acid and zinc on uric acid levels were evaluated. Alterations of the gut microbiota related to hyperuricemia and the treatments were evaluated by sequencing using the Illumina MiSeq system. The results demonstrated that uric acid levels dropped observably, and the activities of adenosine deaminase (ADA) and xanthine oxidase (XOD) were downregulated after folic acid or zinc intervention. 16S rRNA gene sequencing-based gut microbiota analysis revealed that folic acid and zinc enhanced the abundance of probiotic bacteria and reduced that of pathogenic bacteria, thus improving intestinal barrier function. PICRUST analysis indicated that folic acid and zinc restored gut microbiota metabolism. These findings indicate that folic acid and zinc ameliorate hyperuricemia by inhibiting uric acid biosynthesis and stimulating uric acid excretion by modulating the gut microbiota. Thus, folic acid and zinc may be new and safe therapeutic agents to improve hyperuricemia.https://www.frontiersin.org/articles/10.3389/fmicb.2022.907952/fullhyperuricemiauric acidgut microbiotafolic acidzinc |
| spellingShingle | Xuewei Sun Xuewei Sun Jie Wen Jie Wen Baosheng Guan Baosheng Guan Jialin Li Jialin Li Jincheng Luo Jincheng Luo Jie Li Jie Li Mingyu Wei Mingyu Wei Hongbin Qiu Hongbin Qiu Folic acid and zinc improve hyperuricemia by altering the gut microbiota of rats with high-purine diet-induced hyperuricemia Frontiers in Microbiology hyperuricemia uric acid gut microbiota folic acid zinc |
| title | Folic acid and zinc improve hyperuricemia by altering the gut microbiota of rats with high-purine diet-induced hyperuricemia |
| title_full | Folic acid and zinc improve hyperuricemia by altering the gut microbiota of rats with high-purine diet-induced hyperuricemia |
| title_fullStr | Folic acid and zinc improve hyperuricemia by altering the gut microbiota of rats with high-purine diet-induced hyperuricemia |
| title_full_unstemmed | Folic acid and zinc improve hyperuricemia by altering the gut microbiota of rats with high-purine diet-induced hyperuricemia |
| title_short | Folic acid and zinc improve hyperuricemia by altering the gut microbiota of rats with high-purine diet-induced hyperuricemia |
| title_sort | folic acid and zinc improve hyperuricemia by altering the gut microbiota of rats with high purine diet induced hyperuricemia |
| topic | hyperuricemia uric acid gut microbiota folic acid zinc |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.907952/full |
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