Nano Parthenolide Improves Intestinal Barrier Function of Sepsis by Inhibiting Apoptosis and ROS via 5-HTR2A
Ning-Ke Guo,1,2,* Han She,1,* Lei Tan,1 Yuan-Qun Zhou,1 Chun-Qiong Tang,1 Xiao-Yong Peng,1 Chun-Hua Ma,1 Tao Li,1 Liang-Ming Liu1 1State Key Laboratory of Trauma, Burns and Combined Injury, Shock and Transfusion Department, Research Institute of Surgery, Daping Hospital, Army Medical...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2023-02-01
|
| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/nano-parthenolide-improves-intestinal-barrier-function-of-sepsis-by-in-peer-reviewed-fulltext-article-IJN |
| _version_ | 1811165532343238656 |
|---|---|
| author | Guo NK She H Tan L Zhou YQ Tang CQ Peng XY Ma CH Li T Liu LM |
| author_facet | Guo NK She H Tan L Zhou YQ Tang CQ Peng XY Ma CH Li T Liu LM |
| author_sort | Guo NK |
| collection | DOAJ |
| description | Ning-Ke Guo,1,2,* Han She,1,* Lei Tan,1 Yuan-Qun Zhou,1 Chun-Qiong Tang,1 Xiao-Yong Peng,1 Chun-Hua Ma,1 Tao Li,1 Liang-Ming Liu1 1State Key Laboratory of Trauma, Burns and Combined Injury, Shock and Transfusion Department, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, People’s Republic of China; 2The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Tao Li; Liang-Ming Liu, Email lt200132@163.com; lmliu62@tmmu.edu.cnBackground: Intestinal barrier dysfunction is an important complication of sepsis, while the treatment is limited. Recently, parthenolide (PTL) has attracted much attention as a strategy of sepsis, but whether nano parthenolide (Nano PTL) is therapeutic in sepsis-induced intestinal barrier dysfunction is obscured.Methods: In this study, cecal ligation and puncture (CLP)-induced sepsis rats and lipopolysaccharide (LPS)-stimulated intestinal epithelial cells (IECs) were used to investigate the effect of PTL on intestinal barrier dysfunction. Meanwhile, we synthesized Nano PTL and compared the protective effect of Nano PTL with ordinary PTL on intestinal barrier function in septic rats and IECs. Network pharmacology and serotonin 2A (5-HTR2A) inhibitor were used to explore the mechanism of PTL on the intestinal barrier function of sepsis.Results: The encapsulation rate of Nano PTL was 95± 1.5%, the drug loading rate was 11± 0.5%, and the average uptake rate of intestinal epithelial cells was 94%. Ordinary PTL and Nano PTL improved the survival rate and survival time of septic rats, reduced the mean arterial pressure and the serum level of inflammatory cytokines, and protected the liver and kidney functions in vivo, and increased the value of transmembrane resistance (TEER) reduced the reactive oxygen species (ROS) and apoptosis in IECs in vitro through 5-HTR2A. Nano PTL had better effect than ordinary PTL.Conclusion: Ordinary PTL and Nano PTL can protect the intestinal barrier function of septic rats by inhibiting apoptosis and ROS through up-regulating 5-HTR2A, Nano PTL is better than ordinary PTL.Keywords: sepsis, nano PTL, intestinal permeability, 5-HTR2A, apoptosis |
| first_indexed | 2024-04-10T15:38:32Z |
| format | Article |
| id | doaj.art-2521b277146948678d71768333700b35 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-04-10T15:38:32Z |
| publishDate | 2023-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-2521b277146948678d71768333700b352023-02-12T17:45:33ZengDove Medical PressInternational Journal of Nanomedicine1178-20132023-02-01Volume 1869370981575Nano Parthenolide Improves Intestinal Barrier Function of Sepsis by Inhibiting Apoptosis and ROS via 5-HTR2AGuo NKShe HTan LZhou YQTang CQPeng XYMa CHLi TLiu LMNing-Ke Guo,1,2,* Han She,1,* Lei Tan,1 Yuan-Qun Zhou,1 Chun-Qiong Tang,1 Xiao-Yong Peng,1 Chun-Hua Ma,1 Tao Li,1 Liang-Ming Liu1 1State Key Laboratory of Trauma, Burns and Combined Injury, Shock and Transfusion Department, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, People’s Republic of China; 2The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Tao Li; Liang-Ming Liu, Email lt200132@163.com; lmliu62@tmmu.edu.cnBackground: Intestinal barrier dysfunction is an important complication of sepsis, while the treatment is limited. Recently, parthenolide (PTL) has attracted much attention as a strategy of sepsis, but whether nano parthenolide (Nano PTL) is therapeutic in sepsis-induced intestinal barrier dysfunction is obscured.Methods: In this study, cecal ligation and puncture (CLP)-induced sepsis rats and lipopolysaccharide (LPS)-stimulated intestinal epithelial cells (IECs) were used to investigate the effect of PTL on intestinal barrier dysfunction. Meanwhile, we synthesized Nano PTL and compared the protective effect of Nano PTL with ordinary PTL on intestinal barrier function in septic rats and IECs. Network pharmacology and serotonin 2A (5-HTR2A) inhibitor were used to explore the mechanism of PTL on the intestinal barrier function of sepsis.Results: The encapsulation rate of Nano PTL was 95± 1.5%, the drug loading rate was 11± 0.5%, and the average uptake rate of intestinal epithelial cells was 94%. Ordinary PTL and Nano PTL improved the survival rate and survival time of septic rats, reduced the mean arterial pressure and the serum level of inflammatory cytokines, and protected the liver and kidney functions in vivo, and increased the value of transmembrane resistance (TEER) reduced the reactive oxygen species (ROS) and apoptosis in IECs in vitro through 5-HTR2A. Nano PTL had better effect than ordinary PTL.Conclusion: Ordinary PTL and Nano PTL can protect the intestinal barrier function of septic rats by inhibiting apoptosis and ROS through up-regulating 5-HTR2A, Nano PTL is better than ordinary PTL.Keywords: sepsis, nano PTL, intestinal permeability, 5-HTR2A, apoptosishttps://www.dovepress.com/nano-parthenolide-improves-intestinal-barrier-function-of-sepsis-by-in-peer-reviewed-fulltext-article-IJNsepsisnano ptlintestinal permeability;5-htr2aapoptosis |
| spellingShingle | Guo NK She H Tan L Zhou YQ Tang CQ Peng XY Ma CH Li T Liu LM Nano Parthenolide Improves Intestinal Barrier Function of Sepsis by Inhibiting Apoptosis and ROS via 5-HTR2A International Journal of Nanomedicine sepsis nano ptl intestinal permeability;5-htr2a apoptosis |
| title | Nano Parthenolide Improves Intestinal Barrier Function of Sepsis by Inhibiting Apoptosis and ROS via 5-HTR2A |
| title_full | Nano Parthenolide Improves Intestinal Barrier Function of Sepsis by Inhibiting Apoptosis and ROS via 5-HTR2A |
| title_fullStr | Nano Parthenolide Improves Intestinal Barrier Function of Sepsis by Inhibiting Apoptosis and ROS via 5-HTR2A |
| title_full_unstemmed | Nano Parthenolide Improves Intestinal Barrier Function of Sepsis by Inhibiting Apoptosis and ROS via 5-HTR2A |
| title_short | Nano Parthenolide Improves Intestinal Barrier Function of Sepsis by Inhibiting Apoptosis and ROS via 5-HTR2A |
| title_sort | nano parthenolide improves intestinal barrier function of sepsis by inhibiting apoptosis and ros via 5 htr2a |
| topic | sepsis nano ptl intestinal permeability;5-htr2a apoptosis |
| url | https://www.dovepress.com/nano-parthenolide-improves-intestinal-barrier-function-of-sepsis-by-in-peer-reviewed-fulltext-article-IJN |
| work_keys_str_mv | AT guonk nanoparthenolideimprovesintestinalbarrierfunctionofsepsisbyinhibitingapoptosisandrosvia5htr2a AT sheh nanoparthenolideimprovesintestinalbarrierfunctionofsepsisbyinhibitingapoptosisandrosvia5htr2a AT tanl nanoparthenolideimprovesintestinalbarrierfunctionofsepsisbyinhibitingapoptosisandrosvia5htr2a AT zhouyq nanoparthenolideimprovesintestinalbarrierfunctionofsepsisbyinhibitingapoptosisandrosvia5htr2a AT tangcq nanoparthenolideimprovesintestinalbarrierfunctionofsepsisbyinhibitingapoptosisandrosvia5htr2a AT pengxy nanoparthenolideimprovesintestinalbarrierfunctionofsepsisbyinhibitingapoptosisandrosvia5htr2a AT mach nanoparthenolideimprovesintestinalbarrierfunctionofsepsisbyinhibitingapoptosisandrosvia5htr2a AT lit nanoparthenolideimprovesintestinalbarrierfunctionofsepsisbyinhibitingapoptosisandrosvia5htr2a AT liulm nanoparthenolideimprovesintestinalbarrierfunctionofsepsisbyinhibitingapoptosisandrosvia5htr2a |