Effects of dietary n-3 LC-PUFA levels on the growth, immunity, and lipid metabolism of freshwater carnivorous teleost largemouth bass (Micropterus salmoides) juveniles
The n-3 long chain polyunsaturated fatty acids (n-3 LC-PUFA) play important roles in the normal growth and metabolism of fish . In order to explore the appropriate requiremnts of n-3 LC-PUFA in juvenile largemouth bass Micropterus salmoides, a freshwater carnivorous teleost, four isoproteic (50 %) a...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-10-01
|
| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423002430 |
| _version_ | 1797676602091372544 |
|---|---|
| author | Wenqiang An Jianzhao Xu Fang Chen Yongcai Ma Zeliang Su Junfeng Guang Ningning Su Guangrong Zhang Chao Xu Yuanyou Li Dizhi Xie |
| author_facet | Wenqiang An Jianzhao Xu Fang Chen Yongcai Ma Zeliang Su Junfeng Guang Ningning Su Guangrong Zhang Chao Xu Yuanyou Li Dizhi Xie |
| author_sort | Wenqiang An |
| collection | DOAJ |
| description | The n-3 long chain polyunsaturated fatty acids (n-3 LC-PUFA) play important roles in the normal growth and metabolism of fish . In order to explore the appropriate requiremnts of n-3 LC-PUFA in juvenile largemouth bass Micropterus salmoides, a freshwater carnivorous teleost, four isoproteic (50 %) and isolipidic (11 %) diets were formulated. The control diet used fish oil (FO) as the sole lipid source, and the other three diets used mixed vegetable oil as the main lipid source and supplemented with 0.50 %, 0.80 % and 1.10 % n-3 LC-PUFA, respectively. After the juvenile largemouth bass (initial weight 12.11 ± 0.01 g) fed with the four diets for 75 days, the growth, serum and heptic biochemical indices, as well as hepatic lipid metabolism were investigated. The results showed that the growth performance of 0.50 %-1.10 % groups were high that of control group; comparing to the 1.10 % group, high weight gain, specific growth rate and daily gain, as well as muscle hardness, chewiness and gumminess were detected in the 0.80 % group (P < 0.05). With the increase of dietary n-3 LC-PUFA levels, the serum biochemical indices (total cholesterol, triglyceride, and aspartate aminotransferase activity) and hepatic triglyceride were significantly decreased, while the serum acid phosphatase and alkaline phosphatase, as well as hepatic and muscle n-3 LC-PUFA contents were significantly increased (P < 0.05). Compared with the 0.50 % group, high hepatic mRNA expression levels of anti-inflammatory genes (il-10, tgf-β) were observed in the 0.80 % group (P < 0.05); and the relatively high transcript levels of genes related to lipid transport and catabolism (hsl, pparα, fatp and mtp) and low transcript levels of genes related to lipid anabolism (accα and pparγ) were detected in the 0.80 % and 1.10 % groups, as well as high mRNA levels of accα and pparγ were measured in the 0.80 % group (P < 0.05). The results indicated that 0.80 % dietary n-3 LC-PUFA level is suitable for largemouth bass concerning the growth performance, muscle quality and hepatic lipid metabolism. When subjected the weight gain to the second-order polynomial regression analysis, the maximum value was achieved at 0.76 % n-3 LC-PUFA level, which provide a basis for the development of formula feed for largemouth bass. |
| first_indexed | 2024-03-11T22:31:36Z |
| format | Article |
| id | doaj.art-252202476107469e8676cfb77da7a3bd |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-11T22:31:36Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-252202476107469e8676cfb77da7a3bd2023-09-23T05:11:23ZengElsevierAquaculture Reports2352-51342023-10-0132101704Effects of dietary n-3 LC-PUFA levels on the growth, immunity, and lipid metabolism of freshwater carnivorous teleost largemouth bass (Micropterus salmoides) juvenilesWenqiang An0Jianzhao Xu1Fang Chen2Yongcai Ma3Zeliang Su4Junfeng Guang5Ningning Su6Guangrong Zhang7Chao Xu8Yuanyou Li9Dizhi Xie10College of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaCollege of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaCollege of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaCollege of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaCollege of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaCollege of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaCollege of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaCollege of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaCollege of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaCorresponding authors.; College of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaCorresponding authors.; College of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaThe n-3 long chain polyunsaturated fatty acids (n-3 LC-PUFA) play important roles in the normal growth and metabolism of fish . In order to explore the appropriate requiremnts of n-3 LC-PUFA in juvenile largemouth bass Micropterus salmoides, a freshwater carnivorous teleost, four isoproteic (50 %) and isolipidic (11 %) diets were formulated. The control diet used fish oil (FO) as the sole lipid source, and the other three diets used mixed vegetable oil as the main lipid source and supplemented with 0.50 %, 0.80 % and 1.10 % n-3 LC-PUFA, respectively. After the juvenile largemouth bass (initial weight 12.11 ± 0.01 g) fed with the four diets for 75 days, the growth, serum and heptic biochemical indices, as well as hepatic lipid metabolism were investigated. The results showed that the growth performance of 0.50 %-1.10 % groups were high that of control group; comparing to the 1.10 % group, high weight gain, specific growth rate and daily gain, as well as muscle hardness, chewiness and gumminess were detected in the 0.80 % group (P < 0.05). With the increase of dietary n-3 LC-PUFA levels, the serum biochemical indices (total cholesterol, triglyceride, and aspartate aminotransferase activity) and hepatic triglyceride were significantly decreased, while the serum acid phosphatase and alkaline phosphatase, as well as hepatic and muscle n-3 LC-PUFA contents were significantly increased (P < 0.05). Compared with the 0.50 % group, high hepatic mRNA expression levels of anti-inflammatory genes (il-10, tgf-β) were observed in the 0.80 % group (P < 0.05); and the relatively high transcript levels of genes related to lipid transport and catabolism (hsl, pparα, fatp and mtp) and low transcript levels of genes related to lipid anabolism (accα and pparγ) were detected in the 0.80 % and 1.10 % groups, as well as high mRNA levels of accα and pparγ were measured in the 0.80 % group (P < 0.05). The results indicated that 0.80 % dietary n-3 LC-PUFA level is suitable for largemouth bass concerning the growth performance, muscle quality and hepatic lipid metabolism. When subjected the weight gain to the second-order polynomial regression analysis, the maximum value was achieved at 0.76 % n-3 LC-PUFA level, which provide a basis for the development of formula feed for largemouth bass.http://www.sciencedirect.com/science/article/pii/S2352513423002430Largemouth bassN-3 LC-PUFAGrowth performanceMuscle qualityLipid metabolism |
| spellingShingle | Wenqiang An Jianzhao Xu Fang Chen Yongcai Ma Zeliang Su Junfeng Guang Ningning Su Guangrong Zhang Chao Xu Yuanyou Li Dizhi Xie Effects of dietary n-3 LC-PUFA levels on the growth, immunity, and lipid metabolism of freshwater carnivorous teleost largemouth bass (Micropterus salmoides) juveniles Aquaculture Reports Largemouth bass N-3 LC-PUFA Growth performance Muscle quality Lipid metabolism |
| title | Effects of dietary n-3 LC-PUFA levels on the growth, immunity, and lipid metabolism of freshwater carnivorous teleost largemouth bass (Micropterus salmoides) juveniles |
| title_full | Effects of dietary n-3 LC-PUFA levels on the growth, immunity, and lipid metabolism of freshwater carnivorous teleost largemouth bass (Micropterus salmoides) juveniles |
| title_fullStr | Effects of dietary n-3 LC-PUFA levels on the growth, immunity, and lipid metabolism of freshwater carnivorous teleost largemouth bass (Micropterus salmoides) juveniles |
| title_full_unstemmed | Effects of dietary n-3 LC-PUFA levels on the growth, immunity, and lipid metabolism of freshwater carnivorous teleost largemouth bass (Micropterus salmoides) juveniles |
| title_short | Effects of dietary n-3 LC-PUFA levels on the growth, immunity, and lipid metabolism of freshwater carnivorous teleost largemouth bass (Micropterus salmoides) juveniles |
| title_sort | effects of dietary n 3 lc pufa levels on the growth immunity and lipid metabolism of freshwater carnivorous teleost largemouth bass micropterus salmoides juveniles |
| topic | Largemouth bass N-3 LC-PUFA Growth performance Muscle quality Lipid metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423002430 |
| work_keys_str_mv | AT wenqiangan effectsofdietaryn3lcpufalevelsonthegrowthimmunityandlipidmetabolismoffreshwatercarnivorousteleostlargemouthbassmicropterussalmoidesjuveniles AT jianzhaoxu effectsofdietaryn3lcpufalevelsonthegrowthimmunityandlipidmetabolismoffreshwatercarnivorousteleostlargemouthbassmicropterussalmoidesjuveniles AT fangchen effectsofdietaryn3lcpufalevelsonthegrowthimmunityandlipidmetabolismoffreshwatercarnivorousteleostlargemouthbassmicropterussalmoidesjuveniles AT yongcaima effectsofdietaryn3lcpufalevelsonthegrowthimmunityandlipidmetabolismoffreshwatercarnivorousteleostlargemouthbassmicropterussalmoidesjuveniles AT zeliangsu effectsofdietaryn3lcpufalevelsonthegrowthimmunityandlipidmetabolismoffreshwatercarnivorousteleostlargemouthbassmicropterussalmoidesjuveniles AT junfengguang effectsofdietaryn3lcpufalevelsonthegrowthimmunityandlipidmetabolismoffreshwatercarnivorousteleostlargemouthbassmicropterussalmoidesjuveniles AT ningningsu effectsofdietaryn3lcpufalevelsonthegrowthimmunityandlipidmetabolismoffreshwatercarnivorousteleostlargemouthbassmicropterussalmoidesjuveniles AT guangrongzhang effectsofdietaryn3lcpufalevelsonthegrowthimmunityandlipidmetabolismoffreshwatercarnivorousteleostlargemouthbassmicropterussalmoidesjuveniles AT chaoxu effectsofdietaryn3lcpufalevelsonthegrowthimmunityandlipidmetabolismoffreshwatercarnivorousteleostlargemouthbassmicropterussalmoidesjuveniles AT yuanyouli effectsofdietaryn3lcpufalevelsonthegrowthimmunityandlipidmetabolismoffreshwatercarnivorousteleostlargemouthbassmicropterussalmoidesjuveniles AT dizhixie effectsofdietaryn3lcpufalevelsonthegrowthimmunityandlipidmetabolismoffreshwatercarnivorousteleostlargemouthbassmicropterussalmoidesjuveniles |