Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development.
To elucidate metabolism of ascorbic acid (AsA) in sweet cherry fruit (Prunus avium 'Hongdeng'), we quantified AsA concentration, cloned sequences involved in AsA metabolism and investigated their mRNA expression levels, and determined the activity levels of selected enzymes during fruit de...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5330498?pdf=render |
| _version_ | 1818040437310488576 |
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| author | Dong Liang Tingting Zhu Zhiyou Ni Lijin Lin Yi Tang Zhihui Wang Xun Wang Jin Wang Xiulan Lv Hui Xia |
| author_facet | Dong Liang Tingting Zhu Zhiyou Ni Lijin Lin Yi Tang Zhihui Wang Xun Wang Jin Wang Xiulan Lv Hui Xia |
| author_sort | Dong Liang |
| collection | DOAJ |
| description | To elucidate metabolism of ascorbic acid (AsA) in sweet cherry fruit (Prunus avium 'Hongdeng'), we quantified AsA concentration, cloned sequences involved in AsA metabolism and investigated their mRNA expression levels, and determined the activity levels of selected enzymes during fruit development and maturation. We found that AsA concentration was highest at the petal-fall period (0 days after anthesis) and decreased progressively during ripening, but with a slight increase at maturity. AsA did nevertheless continue to accumulate over time because of the increase in fruit fresh weight. Full-length cDNAs of 10 genes involved in the L-galactose pathway of AsA biosynthesis and 10 involved in recycling were obtained. Gene expression patterns of GDP-L-galactose phosphorylase (GGP2), L-galactono-1, 4-lactone dehydrogenase (GalLDH), ascorbate peroxidase (APX3), ascorbate oxidase (AO2), glutathione reductase (GR1), and dehydroascorbate reductase (DHAR1) were in accordance with the AsA concentration pattern during fruit development, indicating that genes involved in ascorbic acid biosynthesis, degradation, and recycling worked in concert to regulate ascorbic acid accumulation in sweet cherry fruit. |
| first_indexed | 2024-12-10T08:14:31Z |
| format | Article |
| id | doaj.art-2522b75c84a64b78803660dd0fca8614 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T08:14:31Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2522b75c84a64b78803660dd0fca86142022-12-22T01:56:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017281810.1371/journal.pone.0172818Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development.Dong LiangTingting ZhuZhiyou NiLijin LinYi TangZhihui WangXun WangJin WangXiulan LvHui XiaTo elucidate metabolism of ascorbic acid (AsA) in sweet cherry fruit (Prunus avium 'Hongdeng'), we quantified AsA concentration, cloned sequences involved in AsA metabolism and investigated their mRNA expression levels, and determined the activity levels of selected enzymes during fruit development and maturation. We found that AsA concentration was highest at the petal-fall period (0 days after anthesis) and decreased progressively during ripening, but with a slight increase at maturity. AsA did nevertheless continue to accumulate over time because of the increase in fruit fresh weight. Full-length cDNAs of 10 genes involved in the L-galactose pathway of AsA biosynthesis and 10 involved in recycling were obtained. Gene expression patterns of GDP-L-galactose phosphorylase (GGP2), L-galactono-1, 4-lactone dehydrogenase (GalLDH), ascorbate peroxidase (APX3), ascorbate oxidase (AO2), glutathione reductase (GR1), and dehydroascorbate reductase (DHAR1) were in accordance with the AsA concentration pattern during fruit development, indicating that genes involved in ascorbic acid biosynthesis, degradation, and recycling worked in concert to regulate ascorbic acid accumulation in sweet cherry fruit.http://europepmc.org/articles/PMC5330498?pdf=render |
| spellingShingle | Dong Liang Tingting Zhu Zhiyou Ni Lijin Lin Yi Tang Zhihui Wang Xun Wang Jin Wang Xiulan Lv Hui Xia Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development. PLoS ONE |
| title | Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development. |
| title_full | Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development. |
| title_fullStr | Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development. |
| title_full_unstemmed | Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development. |
| title_short | Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development. |
| title_sort | ascorbic acid metabolism during sweet cherry prunus avium fruit development |
| url | http://europepmc.org/articles/PMC5330498?pdf=render |
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