Proof of bacteria and the activity of chemical and natural antibiotics by headspace gas chromatography
Abstract An efficient technique was developed to monitor the emission of hydrogen and carbon dioxide from bacteria cultures by headspace gas chromatography and to investigate the efficacy of chemical antibiotics and of natural compounds with antimicrobial properties. Facultative anaerobes emit hydro...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-02-01
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| Series: | Journal of Analytical Science and Technology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40543-019-0167-3 |
| _version_ | 1818850468339646464 |
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| author | Bruno Konrad Kolb Lorina Riesterer Leona Bier Anna-Maria Widenhorn |
| author_facet | Bruno Konrad Kolb Lorina Riesterer Leona Bier Anna-Maria Widenhorn |
| author_sort | Bruno Konrad Kolb |
| collection | DOAJ |
| description | Abstract An efficient technique was developed to monitor the emission of hydrogen and carbon dioxide from bacteria cultures by headspace gas chromatography and to investigate the efficacy of chemical antibiotics and of natural compounds with antimicrobial properties. Facultative anaerobes emit hydrogen and carbon dioxide from cultures in the closed headspace vials and obligate anaerobes too, if the air above the cultures in the vials is replaced by nitrogen. Antibiotics added to the sample cultures are apparently effective if the emission of hydrogen and carbon dioxide is suppressed. If not, either they are ineffective or the related bacteria are even resistant. The headspace technique can detect bacterial contamination in various samples, and examples are presented for food purchased from supermarkets and for medical specimens, including recognition of antibiotic resistance. However, the described technique cannot identify the related bacteria at a species or genus level, but is well suited for example for screening the bulk of food in supermarkets for microbe contamination or for the search of natural antibiotics. The samples are incubated with the proper nutrient medium in closed septum vials which remain closed during the whole process. The personnel in the lab never come into contact with pathogens, and thus, safety regulations are warranted. The described technique can be carried out with all commercially available gas chromatographs, even including simple low-cost instruments, equipped with a standard packed column and a thermal conductivity detector, up to expensive and fully automated instruments, such as used for forensic blood alcohol analysis. |
| first_indexed | 2024-12-19T06:49:37Z |
| format | Article |
| id | doaj.art-2522c41378f542749431309ee4215e1f |
| institution | Directory Open Access Journal |
| issn | 2093-3371 |
| language | English |
| last_indexed | 2024-12-19T06:49:37Z |
| publishDate | 2019-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Analytical Science and Technology |
| spelling | doaj.art-2522c41378f542749431309ee4215e1f2022-12-21T20:31:47ZengSpringerOpenJournal of Analytical Science and Technology2093-33712019-02-011011910.1186/s40543-019-0167-3Proof of bacteria and the activity of chemical and natural antibiotics by headspace gas chromatographyBruno Konrad Kolb0Lorina Riesterer1Leona Bier2Anna-Maria Widenhorn3Student Research Centre, Gymnasium ÜberlingenStudent Research Centre, Gymnasium ÜberlingenStudent Research Centre, Gymnasium ÜberlingenStudent Research Centre, Gymnasium ÜberlingenAbstract An efficient technique was developed to monitor the emission of hydrogen and carbon dioxide from bacteria cultures by headspace gas chromatography and to investigate the efficacy of chemical antibiotics and of natural compounds with antimicrobial properties. Facultative anaerobes emit hydrogen and carbon dioxide from cultures in the closed headspace vials and obligate anaerobes too, if the air above the cultures in the vials is replaced by nitrogen. Antibiotics added to the sample cultures are apparently effective if the emission of hydrogen and carbon dioxide is suppressed. If not, either they are ineffective or the related bacteria are even resistant. The headspace technique can detect bacterial contamination in various samples, and examples are presented for food purchased from supermarkets and for medical specimens, including recognition of antibiotic resistance. However, the described technique cannot identify the related bacteria at a species or genus level, but is well suited for example for screening the bulk of food in supermarkets for microbe contamination or for the search of natural antibiotics. The samples are incubated with the proper nutrient medium in closed septum vials which remain closed during the whole process. The personnel in the lab never come into contact with pathogens, and thus, safety regulations are warranted. The described technique can be carried out with all commercially available gas chromatographs, even including simple low-cost instruments, equipped with a standard packed column and a thermal conductivity detector, up to expensive and fully automated instruments, such as used for forensic blood alcohol analysis.http://link.springer.com/article/10.1186/s40543-019-0167-3BacteriaAntibioticsAntibiotic resistanceHeadspace gas chromatography |
| spellingShingle | Bruno Konrad Kolb Lorina Riesterer Leona Bier Anna-Maria Widenhorn Proof of bacteria and the activity of chemical and natural antibiotics by headspace gas chromatography Journal of Analytical Science and Technology Bacteria Antibiotics Antibiotic resistance Headspace gas chromatography |
| title | Proof of bacteria and the activity of chemical and natural antibiotics by headspace gas chromatography |
| title_full | Proof of bacteria and the activity of chemical and natural antibiotics by headspace gas chromatography |
| title_fullStr | Proof of bacteria and the activity of chemical and natural antibiotics by headspace gas chromatography |
| title_full_unstemmed | Proof of bacteria and the activity of chemical and natural antibiotics by headspace gas chromatography |
| title_short | Proof of bacteria and the activity of chemical and natural antibiotics by headspace gas chromatography |
| title_sort | proof of bacteria and the activity of chemical and natural antibiotics by headspace gas chromatography |
| topic | Bacteria Antibiotics Antibiotic resistance Headspace gas chromatography |
| url | http://link.springer.com/article/10.1186/s40543-019-0167-3 |
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