Efficient assembly of very short oligonucleotides using T4 DNA Ligase

Efficient assembly of very short oligonucleotides using T4 DNA Ligase

<p>Abstract</p> <p>Background</p> <p>In principle, a pre-constructed library of all possible short oligonucleotides could be used to construct many distinct gene sequences. In order to assess the feasibility of such an approach, we characterized T4 DNA Ligase activity o...

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Bibliographic Details
Main Authors: Holt Robert A, Coope Robin JN, Horspool Daniel R
Format: Article
Language:English
Published: BMC 2010-11-01
Series:BMC Research Notes
Online Access:http://www.biomedcentral.com/1756-0500/3/291

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