Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats
This study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activiti...
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| Format: | Article |
| Language: | English |
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Sciendo
2017-12-01
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| Series: | Acta Veterinaria |
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| Online Access: | https://doi.org/10.1515/acve-2017-0043 |
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| author | Popović Nataša Pajović B. Snežana Stojiljković Vesna Todorović Ana Pejić Snežana Pavlović Ivan Gavrilović Ljubica |
| author_facet | Popović Nataša Pajović B. Snežana Stojiljković Vesna Todorović Ana Pejić Snežana Pavlović Ivan Gavrilović Ljubica |
| author_sort | Popović Nataša |
| collection | DOAJ |
| description | This study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantifi ed by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our fi ndings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats. |
| first_indexed | 2024-12-14T08:16:53Z |
| format | Article |
| id | doaj.art-25232fe7505f4866a304f357582b1a01 |
| institution | Directory Open Access Journal |
| issn | 1820-7448 |
| language | English |
| last_indexed | 2024-12-14T08:16:53Z |
| publishDate | 2017-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Veterinaria |
| spelling | doaj.art-25232fe7505f4866a304f357582b1a012022-12-21T23:09:54ZengSciendoActa Veterinaria1820-74482017-12-0167454055010.1515/acve-2017-0043Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed RatsPopović Nataša0Pajović B. Snežana1Stojiljković Vesna2Todorović Ana3Pejić Snežana4Pavlović Ivan5Gavrilović Ljubica6Institute of Nuclear Sciences “Vinča”, Laboratory of Molecular Biology and Endocrinology, University of Belgrade, Belgrade, SerbiaInstitute of Nuclear Sciences “Vinča”, Laboratory of Molecular Biology and Endocrinology, University of Belgrade, Belgrade, SerbiaInstitute of Nuclear Sciences “Vinča”, Laboratory of Molecular Biology and Endocrinology, University of Belgrade, Belgrade, SerbiaInstitute of Nuclear Sciences “Vinča”, Laboratory of Molecular Biology and Endocrinology, University of Belgrade, Belgrade, SerbiaInstitute of Nuclear Sciences “Vinča”, Laboratory of Molecular Biology and Endocrinology, University of Belgrade, Belgrade, SerbiaInstitute of Nuclear Sciences “Vinča”, Laboratory of Molecular Biology and Endocrinology, University of Belgrade, Belgrade, SerbiaInstitute of Nuclear Sciences “Vinča”, Laboratory of Molecular Biology and Endocrinology, University of Belgrade, Belgrade, SerbiaThis study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantifi ed by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our fi ndings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats.https://doi.org/10.1515/acve-2017-0043chronic restraint stresscopper-zinc superoxide dismutasemanganese superoxide dismutasecatalasehippocampusrats |
| spellingShingle | Popović Nataša Pajović B. Snežana Stojiljković Vesna Todorović Ana Pejić Snežana Pavlović Ivan Gavrilović Ljubica Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats Acta Veterinaria chronic restraint stress copper-zinc superoxide dismutase manganese superoxide dismutase catalase hippocampus rats |
| title | Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats |
| title_full | Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats |
| title_fullStr | Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats |
| title_full_unstemmed | Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats |
| title_short | Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats |
| title_sort | increased activity of hippocampal antioxidant enzymes as an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats |
| topic | chronic restraint stress copper-zinc superoxide dismutase manganese superoxide dismutase catalase hippocampus rats |
| url | https://doi.org/10.1515/acve-2017-0043 |
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