Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblasts

Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblasts

Adult human primary dermal fibroblasts [ATCC (PCS-201-012)] were reprogrammed by transfection of oriP/EBNA-1 based episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28 and a p53 shRNA (Okita et al., 2011) to give rise to induced pluripotent stem cells (iPSCs). These iPSCs expressed core plu...

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Bibliographic Details
Main Authors: Khyati Raina, Gaurav Joshi, Kirti Modak, Chitra Premkumar, Sweety Priyanka, Praveena Rajesh, Shaji R. Velayudhan, Rajkumar P. Thummer
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506123001459
Description
Summary:Adult human primary dermal fibroblasts [ATCC (PCS-201-012)] were reprogrammed by transfection of oriP/EBNA-1 based episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28 and a p53 shRNA (Okita et al., 2011) to give rise to induced pluripotent stem cells (iPSCs). These iPSCs expressed core pluripotency markers, maintained normal karyotype, and showed tri-lineage differentiation potential. Further, the absence of episomal plasmid integration in this iPSC line was confirmed by genomic PCR. In addition, DNA fingerprinting of fibroblast and iPSC DNA by microsatellite analysis confirmed the genetic identity of this cell line. This iPSC line was shown to be free from mycoplasma contamination.
ISSN:1873-5061

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