Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblasts
Adult human primary dermal fibroblasts [ATCC (PCS-201-012)] were reprogrammed by transfection of oriP/EBNA-1 based episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28 and a p53 shRNA (Okita et al., 2011) to give rise to induced pluripotent stem cells (iPSCs). These iPSCs expressed core plu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506123001459 |
| _version_ | 1797692034121728000 |
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| author | Khyati Raina Gaurav Joshi Kirti Modak Chitra Premkumar Sweety Priyanka Praveena Rajesh Shaji R. Velayudhan Rajkumar P. Thummer |
| author_facet | Khyati Raina Gaurav Joshi Kirti Modak Chitra Premkumar Sweety Priyanka Praveena Rajesh Shaji R. Velayudhan Rajkumar P. Thummer |
| author_sort | Khyati Raina |
| collection | DOAJ |
| description | Adult human primary dermal fibroblasts [ATCC (PCS-201-012)] were reprogrammed by transfection of oriP/EBNA-1 based episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28 and a p53 shRNA (Okita et al., 2011) to give rise to induced pluripotent stem cells (iPSCs). These iPSCs expressed core pluripotency markers, maintained normal karyotype, and showed tri-lineage differentiation potential. Further, the absence of episomal plasmid integration in this iPSC line was confirmed by genomic PCR. In addition, DNA fingerprinting of fibroblast and iPSC DNA by microsatellite analysis confirmed the genetic identity of this cell line. This iPSC line was shown to be free from mycoplasma contamination. |
| first_indexed | 2024-03-12T02:21:51Z |
| format | Article |
| id | doaj.art-252655a9707f41558f17990806abad32 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-12T02:21:51Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-252655a9707f41558f17990806abad322023-09-06T04:50:49ZengElsevierStem Cell Research1873-50612023-09-0171103159Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblastsKhyati Raina0Gaurav Joshi1Kirti Modak2Chitra Premkumar3Sweety Priyanka4Praveena Rajesh5Shaji R. Velayudhan6Rajkumar P. Thummer7Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, IndiaDepartment of Hematology, Christian Medical College, Vellore, IndiaDepartment of Hematology, Christian Medical College, Vellore, IndiaCenter for Stem Cell Research, Christian Medical College, Vellore, IndiaDepartment of Hematology, Christian Medical College, Vellore, IndiaCenter for Stem Cell Research, Christian Medical College, Vellore, IndiaDepartment of Hematology, Christian Medical College, Vellore, India; Center for Stem Cell Research, Christian Medical College, Vellore, IndiaDepartment of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, India; Corresponding author.Adult human primary dermal fibroblasts [ATCC (PCS-201-012)] were reprogrammed by transfection of oriP/EBNA-1 based episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28 and a p53 shRNA (Okita et al., 2011) to give rise to induced pluripotent stem cells (iPSCs). These iPSCs expressed core pluripotency markers, maintained normal karyotype, and showed tri-lineage differentiation potential. Further, the absence of episomal plasmid integration in this iPSC line was confirmed by genomic PCR. In addition, DNA fingerprinting of fibroblast and iPSC DNA by microsatellite analysis confirmed the genetic identity of this cell line. This iPSC line was shown to be free from mycoplasma contamination.http://www.sciencedirect.com/science/article/pii/S1873506123001459 |
| spellingShingle | Khyati Raina Gaurav Joshi Kirti Modak Chitra Premkumar Sweety Priyanka Praveena Rajesh Shaji R. Velayudhan Rajkumar P. Thummer Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblasts Stem Cell Research |
| title | Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblasts |
| title_full | Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblasts |
| title_fullStr | Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblasts |
| title_full_unstemmed | Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblasts |
| title_short | Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblasts |
| title_sort | generation and characterization of induced pluripotent stem cell line iitgi001 a derived from adult human primary dermal fibroblasts |
| url | http://www.sciencedirect.com/science/article/pii/S1873506123001459 |
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