Potential of sperm small non-coding RNAs as biomarkers of testicular toxicity in a doxorubicin-induced mouse model
Testicular toxicity is a major concern in cancer chemotherapy and drug development as it can result in infertility; however, there are no effective biomarkers for this adverse effect. To identify new biomarkers, we investigated the expression of small non-coding RNAs (sncRNAs) in a mouse model of do...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580821002545 |
| _version_ | 1818353081380765696 |
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| author | Kazuya Sakai Yuki Hiradate Kenshiro Hara Kentaro Tanemura |
| author_facet | Kazuya Sakai Yuki Hiradate Kenshiro Hara Kentaro Tanemura |
| author_sort | Kazuya Sakai |
| collection | DOAJ |
| description | Testicular toxicity is a major concern in cancer chemotherapy and drug development as it can result in infertility; however, there are no effective biomarkers for this adverse effect. To identify new biomarkers, we investigated the expression of small non-coding RNAs (sncRNAs) in a mouse model of doxorubicin (DXR)-induced testicular toxicity. First, we performed small RNA-seq analysis of sperm from DXR-treated or control mice and observed differential expression of many genome-derived sequences. We then performed real-time RT-PCR validation of these sequences and discovered that sncRNA detected by one primers, dxRN_3, showed similar differential expression as that seen in the RNA-seq experiment. These findings suggest that the sncRNAs present in sperm have potential as clinically acceptable biomarkers for testicular toxicity. |
| first_indexed | 2024-12-13T19:03:51Z |
| format | Article |
| id | doaj.art-2526724b6ee142fa81d576a0369793ab |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-13T19:03:51Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-2526724b6ee142fa81d576a0369793ab2022-12-21T23:34:37ZengElsevierBiochemistry and Biophysics Reports2405-58082021-12-0128101160Potential of sperm small non-coding RNAs as biomarkers of testicular toxicity in a doxorubicin-induced mouse modelKazuya Sakai0Yuki Hiradate1Kenshiro Hara2Kentaro Tanemura3Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, JapanLaboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, JapanLaboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, JapanCorresponding author.; Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, JapanTesticular toxicity is a major concern in cancer chemotherapy and drug development as it can result in infertility; however, there are no effective biomarkers for this adverse effect. To identify new biomarkers, we investigated the expression of small non-coding RNAs (sncRNAs) in a mouse model of doxorubicin (DXR)-induced testicular toxicity. First, we performed small RNA-seq analysis of sperm from DXR-treated or control mice and observed differential expression of many genome-derived sequences. We then performed real-time RT-PCR validation of these sequences and discovered that sncRNA detected by one primers, dxRN_3, showed similar differential expression as that seen in the RNA-seq experiment. These findings suggest that the sncRNAs present in sperm have potential as clinically acceptable biomarkers for testicular toxicity.http://www.sciencedirect.com/science/article/pii/S2405580821002545Small non-coding RNAsTesticular toxicityRNA-SequencingDoxorubicinBiomarkersDrug development and chemotherapy |
| spellingShingle | Kazuya Sakai Yuki Hiradate Kenshiro Hara Kentaro Tanemura Potential of sperm small non-coding RNAs as biomarkers of testicular toxicity in a doxorubicin-induced mouse model Biochemistry and Biophysics Reports Small non-coding RNAs Testicular toxicity RNA-Sequencing Doxorubicin Biomarkers Drug development and chemotherapy |
| title | Potential of sperm small non-coding RNAs as biomarkers of testicular toxicity in a doxorubicin-induced mouse model |
| title_full | Potential of sperm small non-coding RNAs as biomarkers of testicular toxicity in a doxorubicin-induced mouse model |
| title_fullStr | Potential of sperm small non-coding RNAs as biomarkers of testicular toxicity in a doxorubicin-induced mouse model |
| title_full_unstemmed | Potential of sperm small non-coding RNAs as biomarkers of testicular toxicity in a doxorubicin-induced mouse model |
| title_short | Potential of sperm small non-coding RNAs as biomarkers of testicular toxicity in a doxorubicin-induced mouse model |
| title_sort | potential of sperm small non coding rnas as biomarkers of testicular toxicity in a doxorubicin induced mouse model |
| topic | Small non-coding RNAs Testicular toxicity RNA-Sequencing Doxorubicin Biomarkers Drug development and chemotherapy |
| url | http://www.sciencedirect.com/science/article/pii/S2405580821002545 |
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