Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus
The morphological and molecular characterisations of two lance nematode species isolated from the rhizosphere of banana, Hoplolaimus seinhorsti and H. pararobustus, are provided based on an integrative study that includes light and scanning electron microscopy, phylogenetic analysis and two tree-bas...
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| Format: | Article |
| Language: | English |
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Sciendo
2023-05-01
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| Series: | Journal of Nematology |
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| Online Access: | https://doi.org/10.2478/jofnem-2023-0019 |
| _version_ | 1797219102149836800 |
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| author | Olajide Emmanuel Singh Phougeishangbam Rolish Kolombia Yao A. Rumbarar Merlin Kornelia Couvreur Marjolein Bert Wim |
| author_facet | Olajide Emmanuel Singh Phougeishangbam Rolish Kolombia Yao A. Rumbarar Merlin Kornelia Couvreur Marjolein Bert Wim |
| author_sort | Olajide Emmanuel |
| collection | DOAJ |
| description | The morphological and molecular characterisations of two lance nematode species isolated from the rhizosphere of banana, Hoplolaimus seinhorsti and H. pararobustus, are provided based on an integrative study that includes light and scanning electron microscopy, phylogenetic analysis and two tree-based molecular species delimitation methods (GMYC and bPTP). Nineteen new sequences were obtained, including 5 partial 18S rRNA, 6 D2-D3 of 28S rRNA, 1 ITS rRNA and 7 COI mtDNA (the first COI sequences of H. seinhorsti and H. pararobustus), and an updated morphological character comparison of 37 Hoplolaimus species is presented. The tree-based molecular species-delimitation approaches employed gave markedly differing results, and also showed remarkable discrepancies among the investigated genes, although the bPTP output was found to agree well with established morphological species delimitations. Both species-delimitation approaches did, however, provide the same output for the COI mtDNA sequences, and the COI mtDNA gene sequence was also found to correspond better to established morphological species. It is therefore recommended by this paper as representing the most suitable barcode marker for Hoplolaimus species identification. This integrative study also resulted in the corrective reassignment of 17 gene sequences that were previously unidentified or incorrectly classified, as well as concluding that H. pararobustus consists of two cryptic species. |
| first_indexed | 2024-03-08T10:02:30Z |
| format | Article |
| id | doaj.art-2527312e1b21437da95fa5c42d947213 |
| institution | Directory Open Access Journal |
| issn | 2640-396X |
| language | English |
| last_indexed | 2024-04-24T12:28:18Z |
| publishDate | 2023-05-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Nematology |
| spelling | doaj.art-2527312e1b21437da95fa5c42d9472132024-04-08T07:37:21ZengSciendoJournal of Nematology2640-396X2023-05-0155114715210.2478/jofnem-2023-0019Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus HoplolaimusOlajide Emmanuel0Singh Phougeishangbam Rolish1Kolombia Yao A.2Rumbarar Merlin Kornelia3Couvreur Marjolein4Bert Wim51Nematology Research Unit, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 9000Ghent, Belgium1Nematology Research Unit, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 9000Ghent, Belgium2International Institute of Tropical Agriculture (IITA), Head Quarters and West Africa Hub, P.M.B. 5320, Oyo Road, Ibadan200001, Oyo State, Nigeria1Nematology Research Unit, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 9000Ghent, Belgium1Nematology Research Unit, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 9000Ghent, Belgium1Nematology Research Unit, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 9000Ghent, BelgiumThe morphological and molecular characterisations of two lance nematode species isolated from the rhizosphere of banana, Hoplolaimus seinhorsti and H. pararobustus, are provided based on an integrative study that includes light and scanning electron microscopy, phylogenetic analysis and two tree-based molecular species delimitation methods (GMYC and bPTP). Nineteen new sequences were obtained, including 5 partial 18S rRNA, 6 D2-D3 of 28S rRNA, 1 ITS rRNA and 7 COI mtDNA (the first COI sequences of H. seinhorsti and H. pararobustus), and an updated morphological character comparison of 37 Hoplolaimus species is presented. The tree-based molecular species-delimitation approaches employed gave markedly differing results, and also showed remarkable discrepancies among the investigated genes, although the bPTP output was found to agree well with established morphological species delimitations. Both species-delimitation approaches did, however, provide the same output for the COI mtDNA sequences, and the COI mtDNA gene sequence was also found to correspond better to established morphological species. It is therefore recommended by this paper as representing the most suitable barcode marker for Hoplolaimus species identification. This integrative study also resulted in the corrective reassignment of 17 gene sequences that were previously unidentified or incorrectly classified, as well as concluding that H. pararobustus consists of two cryptic species.https://doi.org/10.2478/jofnem-2023-001928s rdnaits rdnacoibananahoplolaimuslance nematodemorphologyphylogenyplant-parasitic nematodescanning electron microscopysystematictaxonomy |
| spellingShingle | Olajide Emmanuel Singh Phougeishangbam Rolish Kolombia Yao A. Rumbarar Merlin Kornelia Couvreur Marjolein Bert Wim Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus Journal of Nematology 28s rdna its rdna coi banana hoplolaimus lance nematode morphology phylogeny plant-parasitic nematode scanning electron microscopy systematic taxonomy |
| title | Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus |
| title_full | Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus |
| title_fullStr | Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus |
| title_full_unstemmed | Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus |
| title_short | Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus |
| title_sort | characterization of hoplolaimus seinhorsti and hoplolaimus pararobustus tylenchina hoplolaimidae from banana with phylogeny and species delineation in the genus hoplolaimus |
| topic | 28s rdna its rdna coi banana hoplolaimus lance nematode morphology phylogeny plant-parasitic nematode scanning electron microscopy systematic taxonomy |
| url | https://doi.org/10.2478/jofnem-2023-0019 |
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