Biological Control of Pear Valsa Canker Caused by <i>Valsa pyri</i> Using <i>Penicillium</i> <i>citrinum</i>
Valsa canker caused by <i>Valsa pyri</i> is one of the most destructive diseases of commercial pear. For the present analysis, 29 different endophytic fungal strains were isolated from the branches of a healthy pear tree. In dual culture assays, strain ZZ1 exhibited robust antifungal act...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/7/7/198 |
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| author | Hongbo Yuan Bingke Shi Tianxiang Huang Zengqiang Zhou Li Wang Hui Hou Hongtao Tu |
| author_facet | Hongbo Yuan Bingke Shi Tianxiang Huang Zengqiang Zhou Li Wang Hui Hou Hongtao Tu |
| author_sort | Hongbo Yuan |
| collection | DOAJ |
| description | Valsa canker caused by <i>Valsa pyri</i> is one of the most destructive diseases of commercial pear. For the present analysis, 29 different endophytic fungal strains were isolated from the branches of a healthy pear tree. In dual culture assays, strain ZZ1 exhibited robust antifungal activity against all tested pathogens including <i>Valsa pyri</i>. Microscopic analyses suggested that following co-culture with ZZ1, the hyphae of <i>V. pyri</i> were ragged, thin, and ruptured. ZZ1 also induced significant decreases in lesion length and disease incidence on detached pear branches inoculated with <i>V. pyri</i>. ZZ1 isolate-derived culture filtrates also exhibited antifungal activity against <i>V. pyri</i>, decreasing mycelial growth and conidium germination and inhibiting <i>V. pyri-</i>associated lesion development on pear branches. These results suggest that the ZZ1 isolate has the potential for use as a biological control agent against <i>V. pyri</i>. The strain was further identified as <i>Penicillium</i> <i>citrinum</i> based on its morphological characteristics and molecular analyses. Overall, these data highlight a potentially valuable new biocontrol resource for combating pear Valsa canker. |
| first_indexed | 2024-03-10T09:37:59Z |
| format | Article |
| id | doaj.art-252864bd7e654fb1893ae2cb6a6758b5 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T09:37:59Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-252864bd7e654fb1893ae2cb6a6758b52023-11-22T03:53:49ZengMDPI AGHorticulturae2311-75242021-07-017719810.3390/horticulturae7070198Biological Control of Pear Valsa Canker Caused by <i>Valsa pyri</i> Using <i>Penicillium</i> <i>citrinum</i>Hongbo Yuan0Bingke Shi1Tianxiang Huang2Zengqiang Zhou3Li Wang4Hui Hou5Hongtao Tu6Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaValsa canker caused by <i>Valsa pyri</i> is one of the most destructive diseases of commercial pear. For the present analysis, 29 different endophytic fungal strains were isolated from the branches of a healthy pear tree. In dual culture assays, strain ZZ1 exhibited robust antifungal activity against all tested pathogens including <i>Valsa pyri</i>. Microscopic analyses suggested that following co-culture with ZZ1, the hyphae of <i>V. pyri</i> were ragged, thin, and ruptured. ZZ1 also induced significant decreases in lesion length and disease incidence on detached pear branches inoculated with <i>V. pyri</i>. ZZ1 isolate-derived culture filtrates also exhibited antifungal activity against <i>V. pyri</i>, decreasing mycelial growth and conidium germination and inhibiting <i>V. pyri-</i>associated lesion development on pear branches. These results suggest that the ZZ1 isolate has the potential for use as a biological control agent against <i>V. pyri</i>. The strain was further identified as <i>Penicillium</i> <i>citrinum</i> based on its morphological characteristics and molecular analyses. Overall, these data highlight a potentially valuable new biocontrol resource for combating pear Valsa canker.https://www.mdpi.com/2311-7524/7/7/198Valsa canker<i>Valsa pyri</i>biological controlantifungal activity<i>Penicillium citrinum</i> |
| spellingShingle | Hongbo Yuan Bingke Shi Tianxiang Huang Zengqiang Zhou Li Wang Hui Hou Hongtao Tu Biological Control of Pear Valsa Canker Caused by <i>Valsa pyri</i> Using <i>Penicillium</i> <i>citrinum</i> Horticulturae Valsa canker <i>Valsa pyri</i> biological control antifungal activity <i>Penicillium citrinum</i> |
| title | Biological Control of Pear Valsa Canker Caused by <i>Valsa pyri</i> Using <i>Penicillium</i> <i>citrinum</i> |
| title_full | Biological Control of Pear Valsa Canker Caused by <i>Valsa pyri</i> Using <i>Penicillium</i> <i>citrinum</i> |
| title_fullStr | Biological Control of Pear Valsa Canker Caused by <i>Valsa pyri</i> Using <i>Penicillium</i> <i>citrinum</i> |
| title_full_unstemmed | Biological Control of Pear Valsa Canker Caused by <i>Valsa pyri</i> Using <i>Penicillium</i> <i>citrinum</i> |
| title_short | Biological Control of Pear Valsa Canker Caused by <i>Valsa pyri</i> Using <i>Penicillium</i> <i>citrinum</i> |
| title_sort | biological control of pear valsa canker caused by i valsa pyri i using i penicillium i i citrinum i |
| topic | Valsa canker <i>Valsa pyri</i> biological control antifungal activity <i>Penicillium citrinum</i> |
| url | https://www.mdpi.com/2311-7524/7/7/198 |
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