Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses
<p>Abstract</p> <p>Background</p> <p>Human T-cell leukemia virus type 1 (HTLV-1) and type 2 both target T lymphocytes, yet induce radically different phenotypic outcomes. HTLV-1 is a causative agent of Adult T-cell leukemia (ATL), whereas HTLV-2, highly similar to HTLV-...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-03-01
|
| Series: | Retrovirology |
| Subjects: | |
| Online Access: | http://www.retrovirology.com/content/9/1/26 |
| _version_ | 1818066346703847424 |
|---|---|
| author | Simonis Nicolas Rual Jean-François Lemmens Irma Boxus Mathieu Hirozane-Kishikawa Tomoko Gatot Jean-Stéphane Dricot Amélie Hao Tong Vertommen Didier Legros Sébastien Daakour Sarah Klitgord Niels Martin Maud Willaert Jean-François Dequiedt Franck Navratil Vincent Cusick Michael E Burny Arsène Van Lint Carine Hill David E Tavernier Jan Kettmann Richard Vidal Marc Twizere Jean-Claude |
| author_facet | Simonis Nicolas Rual Jean-François Lemmens Irma Boxus Mathieu Hirozane-Kishikawa Tomoko Gatot Jean-Stéphane Dricot Amélie Hao Tong Vertommen Didier Legros Sébastien Daakour Sarah Klitgord Niels Martin Maud Willaert Jean-François Dequiedt Franck Navratil Vincent Cusick Michael E Burny Arsène Van Lint Carine Hill David E Tavernier Jan Kettmann Richard Vidal Marc Twizere Jean-Claude |
| author_sort | Simonis Nicolas |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Human T-cell leukemia virus type 1 (HTLV-1) and type 2 both target T lymphocytes, yet induce radically different phenotypic outcomes. HTLV-1 is a causative agent of Adult T-cell leukemia (ATL), whereas HTLV-2, highly similar to HTLV-1, causes no known overt disease. HTLV gene products are engaged in a dynamic struggle of activating and antagonistic interactions with host cells. Investigations focused on one or a few genes have identified several human factors interacting with HTLV viral proteins. Most of the available interaction data concern the highly investigated HTLV-1 Tax protein. Identifying shared and distinct host-pathogen protein interaction profiles for these two viruses would enlighten how they exploit distinctive or common strategies to subvert cellular pathways toward disease progression.</p> <p>Results</p> <p>We employ a scalable methodology for the systematic mapping and comparison of pathogen-host protein interactions that includes stringent yeast two-hybrid screening and systematic retest, as well as two independent validations through an additional protein interaction detection method and a functional transactivation assay. The final data set contained 166 interactions between 10 viral proteins and 122 human proteins. Among the 166 interactions identified, 87 and 79 involved HTLV-1 and HTLV-2 -encoded proteins, respectively. Targets for HTLV-1 and HTLV-2 proteins implicate a diverse set of cellular processes including the ubiquitin-proteasome system, the apoptosis, different cancer pathways and the Notch signaling pathway.</p> <p>Conclusions</p> <p>This study constitutes a first pass, with homogeneous data, at comparative analysis of host targets for HTLV-1 and -2 retroviruses, complements currently existing data for formulation of systems biology models of retroviral induced diseases and presents new insights on biological pathways involved in retroviral infection.</p> |
| first_indexed | 2024-12-10T15:06:20Z |
| format | Article |
| id | doaj.art-2531aa0f51634cccb86e6e3a3db3decb |
| institution | Directory Open Access Journal |
| issn | 1742-4690 |
| language | English |
| last_indexed | 2024-12-10T15:06:20Z |
| publishDate | 2012-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Retrovirology |
| spelling | doaj.art-2531aa0f51634cccb86e6e3a3db3decb2022-12-22T01:44:02ZengBMCRetrovirology1742-46902012-03-01912610.1186/1742-4690-9-26Host-pathogen interactome mapping for HTLV-1 and -2 retrovirusesSimonis NicolasRual Jean-FrançoisLemmens IrmaBoxus MathieuHirozane-Kishikawa TomokoGatot Jean-StéphaneDricot AmélieHao TongVertommen DidierLegros SébastienDaakour SarahKlitgord NielsMartin MaudWillaert Jean-FrançoisDequiedt FranckNavratil VincentCusick Michael EBurny ArsèneVan Lint CarineHill David ETavernier JanKettmann RichardVidal MarcTwizere Jean-Claude<p>Abstract</p> <p>Background</p> <p>Human T-cell leukemia virus type 1 (HTLV-1) and type 2 both target T lymphocytes, yet induce radically different phenotypic outcomes. HTLV-1 is a causative agent of Adult T-cell leukemia (ATL), whereas HTLV-2, highly similar to HTLV-1, causes no known overt disease. HTLV gene products are engaged in a dynamic struggle of activating and antagonistic interactions with host cells. Investigations focused on one or a few genes have identified several human factors interacting with HTLV viral proteins. Most of the available interaction data concern the highly investigated HTLV-1 Tax protein. Identifying shared and distinct host-pathogen protein interaction profiles for these two viruses would enlighten how they exploit distinctive or common strategies to subvert cellular pathways toward disease progression.</p> <p>Results</p> <p>We employ a scalable methodology for the systematic mapping and comparison of pathogen-host protein interactions that includes stringent yeast two-hybrid screening and systematic retest, as well as two independent validations through an additional protein interaction detection method and a functional transactivation assay. The final data set contained 166 interactions between 10 viral proteins and 122 human proteins. Among the 166 interactions identified, 87 and 79 involved HTLV-1 and HTLV-2 -encoded proteins, respectively. Targets for HTLV-1 and HTLV-2 proteins implicate a diverse set of cellular processes including the ubiquitin-proteasome system, the apoptosis, different cancer pathways and the Notch signaling pathway.</p> <p>Conclusions</p> <p>This study constitutes a first pass, with homogeneous data, at comparative analysis of host targets for HTLV-1 and -2 retroviruses, complements currently existing data for formulation of systems biology models of retroviral induced diseases and presents new insights on biological pathways involved in retroviral infection.</p>http://www.retrovirology.com/content/9/1/26HTLVInteractomeRetrovirusORFeomeTaxHBZ |
| spellingShingle | Simonis Nicolas Rual Jean-François Lemmens Irma Boxus Mathieu Hirozane-Kishikawa Tomoko Gatot Jean-Stéphane Dricot Amélie Hao Tong Vertommen Didier Legros Sébastien Daakour Sarah Klitgord Niels Martin Maud Willaert Jean-François Dequiedt Franck Navratil Vincent Cusick Michael E Burny Arsène Van Lint Carine Hill David E Tavernier Jan Kettmann Richard Vidal Marc Twizere Jean-Claude Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses Retrovirology HTLV Interactome Retrovirus ORFeome Tax HBZ |
| title | Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses |
| title_full | Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses |
| title_fullStr | Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses |
| title_full_unstemmed | Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses |
| title_short | Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses |
| title_sort | host pathogen interactome mapping for htlv 1 and 2 retroviruses |
| topic | HTLV Interactome Retrovirus ORFeome Tax HBZ |
| url | http://www.retrovirology.com/content/9/1/26 |
| work_keys_str_mv | AT simonisnicolas hostpathogeninteractomemappingforhtlv1and2retroviruses AT rualjeanfrancois hostpathogeninteractomemappingforhtlv1and2retroviruses AT lemmensirma hostpathogeninteractomemappingforhtlv1and2retroviruses AT boxusmathieu hostpathogeninteractomemappingforhtlv1and2retroviruses AT hirozanekishikawatomoko hostpathogeninteractomemappingforhtlv1and2retroviruses AT gatotjeanstephane hostpathogeninteractomemappingforhtlv1and2retroviruses AT dricotamelie hostpathogeninteractomemappingforhtlv1and2retroviruses AT haotong hostpathogeninteractomemappingforhtlv1and2retroviruses AT vertommendidier hostpathogeninteractomemappingforhtlv1and2retroviruses AT legrossebastien hostpathogeninteractomemappingforhtlv1and2retroviruses AT daakoursarah hostpathogeninteractomemappingforhtlv1and2retroviruses AT klitgordniels hostpathogeninteractomemappingforhtlv1and2retroviruses AT martinmaud hostpathogeninteractomemappingforhtlv1and2retroviruses AT willaertjeanfrancois hostpathogeninteractomemappingforhtlv1and2retroviruses AT dequiedtfranck hostpathogeninteractomemappingforhtlv1and2retroviruses AT navratilvincent hostpathogeninteractomemappingforhtlv1and2retroviruses AT cusickmichaele hostpathogeninteractomemappingforhtlv1and2retroviruses AT burnyarsene hostpathogeninteractomemappingforhtlv1and2retroviruses AT vanlintcarine hostpathogeninteractomemappingforhtlv1and2retroviruses AT hilldavide hostpathogeninteractomemappingforhtlv1and2retroviruses AT tavernierjan hostpathogeninteractomemappingforhtlv1and2retroviruses AT kettmannrichard hostpathogeninteractomemappingforhtlv1and2retroviruses AT vidalmarc hostpathogeninteractomemappingforhtlv1and2retroviruses AT twizerejeanclaude hostpathogeninteractomemappingforhtlv1and2retroviruses |