Deciphering Aphanomyces euteiches-pea-biocontrol bacterium interactions through untargeted metabolomics
Abstract Aphanomyces euteiches causes root rot in pea, leading to significant yield losses. However, the metabolites involved in this pathosystem have not been thoroughly studied. This study aimed to fill this gap and explore mechanisms of bacterial suppression of A. euteiches via untargeted metabol...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52949-w |
| _version_ | 1797199448792629248 |
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| author | Zakir Hossain Shuang Zhao Kui Liu Liang Li Michelle Hubbard |
| author_facet | Zakir Hossain Shuang Zhao Kui Liu Liang Li Michelle Hubbard |
| author_sort | Zakir Hossain |
| collection | DOAJ |
| description | Abstract Aphanomyces euteiches causes root rot in pea, leading to significant yield losses. However, the metabolites involved in this pathosystem have not been thoroughly studied. This study aimed to fill this gap and explore mechanisms of bacterial suppression of A. euteiches via untargeted metabolomics using pea grown in a controlled environment. Chemical isotope labeling (CIL), followed by liquid chromatography-mass spectrometry (LC–MS), was used for metabolite separation and detection. Univariate and multivariate analyses showed clear separation of metabolites from pathogen-treated pea roots and roots from other treatments. A three-tier approach positively or putatively identified 5249 peak pairs or metabolites. Of these, 403 were positively identified in tier 1; 940 were putatively identified with high confidence in tier 2. There were substantial changes in amino acid pool, and fatty acid and phenylpropanoid pathway products. More metabolites, including salicylic and jasmonic acids, were upregulated than downregulated in A. euteiches-infected roots. 1-aminocyclopropane-1-carboxylic acid and 12-oxophytodienoic acid were upregulated in A. euteiches + bacterium-treated roots compared to A. euteiches-infected roots. A great number of metabolites were up- or down-regulated in response to A. euteiches infection compared with the control and A. euteiches + bacterium-treated plants. The results of this study could facilitate improved disease management. |
| first_indexed | 2024-04-24T07:15:55Z |
| format | Article |
| id | doaj.art-25323ff64787407da6d0a043036ac4a1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T07:15:55Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-25323ff64787407da6d0a043036ac4a12024-04-21T11:16:55ZengNature PortfolioScientific Reports2045-23222024-04-0114111610.1038/s41598-024-52949-wDeciphering Aphanomyces euteiches-pea-biocontrol bacterium interactions through untargeted metabolomicsZakir Hossain0Shuang Zhao1Kui Liu2Liang Li3Michelle Hubbard4Swift Current Research and Development Centre, Agriculture and Agri-Food CanadaDepartment of Chemistry, University of AlbertaSwift Current Research and Development Centre, Agriculture and Agri-Food CanadaDepartment of Chemistry, University of AlbertaSwift Current Research and Development Centre, Agriculture and Agri-Food CanadaAbstract Aphanomyces euteiches causes root rot in pea, leading to significant yield losses. However, the metabolites involved in this pathosystem have not been thoroughly studied. This study aimed to fill this gap and explore mechanisms of bacterial suppression of A. euteiches via untargeted metabolomics using pea grown in a controlled environment. Chemical isotope labeling (CIL), followed by liquid chromatography-mass spectrometry (LC–MS), was used for metabolite separation and detection. Univariate and multivariate analyses showed clear separation of metabolites from pathogen-treated pea roots and roots from other treatments. A three-tier approach positively or putatively identified 5249 peak pairs or metabolites. Of these, 403 were positively identified in tier 1; 940 were putatively identified with high confidence in tier 2. There were substantial changes in amino acid pool, and fatty acid and phenylpropanoid pathway products. More metabolites, including salicylic and jasmonic acids, were upregulated than downregulated in A. euteiches-infected roots. 1-aminocyclopropane-1-carboxylic acid and 12-oxophytodienoic acid were upregulated in A. euteiches + bacterium-treated roots compared to A. euteiches-infected roots. A great number of metabolites were up- or down-regulated in response to A. euteiches infection compared with the control and A. euteiches + bacterium-treated plants. The results of this study could facilitate improved disease management.https://doi.org/10.1038/s41598-024-52949-w |
| spellingShingle | Zakir Hossain Shuang Zhao Kui Liu Liang Li Michelle Hubbard Deciphering Aphanomyces euteiches-pea-biocontrol bacterium interactions through untargeted metabolomics Scientific Reports |
| title | Deciphering Aphanomyces euteiches-pea-biocontrol bacterium interactions through untargeted metabolomics |
| title_full | Deciphering Aphanomyces euteiches-pea-biocontrol bacterium interactions through untargeted metabolomics |
| title_fullStr | Deciphering Aphanomyces euteiches-pea-biocontrol bacterium interactions through untargeted metabolomics |
| title_full_unstemmed | Deciphering Aphanomyces euteiches-pea-biocontrol bacterium interactions through untargeted metabolomics |
| title_short | Deciphering Aphanomyces euteiches-pea-biocontrol bacterium interactions through untargeted metabolomics |
| title_sort | deciphering aphanomyces euteiches pea biocontrol bacterium interactions through untargeted metabolomics |
| url | https://doi.org/10.1038/s41598-024-52949-w |
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