Genetic Diversity and Population Structure of <i>Leptosphaeria biglobosa</i> from the Winter Oilseed Rape Region in China
Phoma stem canker (blackleg), caused by the fungi <i>Leptosphaeria maculans</i> (anamorph <i>Phoma lingam</i>) and <i>L. biglobosa</i>, is one of the most devastating diseases in oilseed rape (<i>Brassica napus</i> L.) production worldwide. However, th...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/9/11/1092 |
| _version_ | 1797458696430682112 |
|---|---|
| author | Kang Zhou Jing Zhang Long Yang Guoqing Li Mingde Wu |
| author_facet | Kang Zhou Jing Zhang Long Yang Guoqing Li Mingde Wu |
| author_sort | Kang Zhou |
| collection | DOAJ |
| description | Phoma stem canker (blackleg), caused by the fungi <i>Leptosphaeria maculans</i> (anamorph <i>Phoma lingam</i>) and <i>L. biglobosa</i>, is one of the most devastating diseases in oilseed rape (<i>Brassica napus</i> L.) production worldwide. However, the population structure and genetic variation of <i>L. biglobosa</i> populations in China have rarely been investigated. Here, a collection of 214 fungal strains of blackleg from China (including Shaanxi, Jiangxi, Hubei, Jiangsu, Chongqing, Sichuan, Guangxi, Guizhou, Hunan, and Henan) and Europe (France and Ukraine) was identified as <i>L. biglobosa</i>. Three simple sequence repeat (SSR) markers were developed to characterize their population structure. The results showed that the Nei’s average gene diversity ranged from 0.6771 for the population from Jiangsu to 0.3009 for the population from Hunan. In addition, most of the genetic variability (96%) occurred within groups and there were only relatively small amounts among groups (4.0%) (<i>F<sub>ST</sub></i> = 0.043, <i>p</i> = 0.042 < 0.05). Pairwise population differentiation (<i>F<sub>ST</sub></i>) suggested that significant genetic differentiation was observed between different <i>L. biglobosa</i> populations. Bayesian and unweighted average method analysis revealed that these <i>L. biglobosa</i> strains were clustered into three branches, and three European strains were similar to those from eastern China. The pathogenicity assay showed that those in Group III were significantly more virulent than those in Group I (t = 2.69, <i>p</i> = 0.016). The study also showed that Group III was dominant in Chinese <i>L. biglobosa</i> populations, which provides new insights for the further study of population evolution and the management of this pathogen. |
| first_indexed | 2024-03-09T16:41:47Z |
| format | Article |
| id | doaj.art-253579e0fcde434b9c5fa07b4ca85952 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T16:41:47Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-253579e0fcde434b9c5fa07b4ca859522023-11-24T14:51:07ZengMDPI AGJournal of Fungi2309-608X2023-11-01911109210.3390/jof9111092Genetic Diversity and Population Structure of <i>Leptosphaeria biglobosa</i> from the Winter Oilseed Rape Region in ChinaKang Zhou0Jing Zhang1Long Yang2Guoqing Li3Mingde Wu4The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaThe State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaThe State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaThe State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaThe State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaPhoma stem canker (blackleg), caused by the fungi <i>Leptosphaeria maculans</i> (anamorph <i>Phoma lingam</i>) and <i>L. biglobosa</i>, is one of the most devastating diseases in oilseed rape (<i>Brassica napus</i> L.) production worldwide. However, the population structure and genetic variation of <i>L. biglobosa</i> populations in China have rarely been investigated. Here, a collection of 214 fungal strains of blackleg from China (including Shaanxi, Jiangxi, Hubei, Jiangsu, Chongqing, Sichuan, Guangxi, Guizhou, Hunan, and Henan) and Europe (France and Ukraine) was identified as <i>L. biglobosa</i>. Three simple sequence repeat (SSR) markers were developed to characterize their population structure. The results showed that the Nei’s average gene diversity ranged from 0.6771 for the population from Jiangsu to 0.3009 for the population from Hunan. In addition, most of the genetic variability (96%) occurred within groups and there were only relatively small amounts among groups (4.0%) (<i>F<sub>ST</sub></i> = 0.043, <i>p</i> = 0.042 < 0.05). Pairwise population differentiation (<i>F<sub>ST</sub></i>) suggested that significant genetic differentiation was observed between different <i>L. biglobosa</i> populations. Bayesian and unweighted average method analysis revealed that these <i>L. biglobosa</i> strains were clustered into three branches, and three European strains were similar to those from eastern China. The pathogenicity assay showed that those in Group III were significantly more virulent than those in Group I (t = 2.69, <i>p</i> = 0.016). The study also showed that Group III was dominant in Chinese <i>L. biglobosa</i> populations, which provides new insights for the further study of population evolution and the management of this pathogen.https://www.mdpi.com/2309-608X/9/11/1092blackleg<i>Leptosphaeria biglobosa</i>genetic diversitypopulation structurepathogenicity |
| spellingShingle | Kang Zhou Jing Zhang Long Yang Guoqing Li Mingde Wu Genetic Diversity and Population Structure of <i>Leptosphaeria biglobosa</i> from the Winter Oilseed Rape Region in China Journal of Fungi blackleg <i>Leptosphaeria biglobosa</i> genetic diversity population structure pathogenicity |
| title | Genetic Diversity and Population Structure of <i>Leptosphaeria biglobosa</i> from the Winter Oilseed Rape Region in China |
| title_full | Genetic Diversity and Population Structure of <i>Leptosphaeria biglobosa</i> from the Winter Oilseed Rape Region in China |
| title_fullStr | Genetic Diversity and Population Structure of <i>Leptosphaeria biglobosa</i> from the Winter Oilseed Rape Region in China |
| title_full_unstemmed | Genetic Diversity and Population Structure of <i>Leptosphaeria biglobosa</i> from the Winter Oilseed Rape Region in China |
| title_short | Genetic Diversity and Population Structure of <i>Leptosphaeria biglobosa</i> from the Winter Oilseed Rape Region in China |
| title_sort | genetic diversity and population structure of i leptosphaeria biglobosa i from the winter oilseed rape region in china |
| topic | blackleg <i>Leptosphaeria biglobosa</i> genetic diversity population structure pathogenicity |
| url | https://www.mdpi.com/2309-608X/9/11/1092 |
| work_keys_str_mv | AT kangzhou geneticdiversityandpopulationstructureofileptosphaeriabiglobosaifromthewinteroilseedraperegioninchina AT jingzhang geneticdiversityandpopulationstructureofileptosphaeriabiglobosaifromthewinteroilseedraperegioninchina AT longyang geneticdiversityandpopulationstructureofileptosphaeriabiglobosaifromthewinteroilseedraperegioninchina AT guoqingli geneticdiversityandpopulationstructureofileptosphaeriabiglobosaifromthewinteroilseedraperegioninchina AT mingdewu geneticdiversityandpopulationstructureofileptosphaeriabiglobosaifromthewinteroilseedraperegioninchina |