Quantitative Study of the Effect of Nanoparticles on the Mechanical Properties of Colon Cancer Cells
Magnetic nanoparticles (MNPs) are often used as drug-carrying particles for targeted therapy of tumors. Studying their effects on cell activity and mechanical properties is of great significance for the targeted treatment of tumors. In this paper, we used a combination of atomic force microscopy (AF...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2023/05/bioconf_bfs2023_01007.pdf |
| _version_ | 1797808039319830528 |
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| author | Cui Haobo Xu Hongmei Li Jingxin Zhang Ziyu Liu Xuwei Yuan Lixin Wang Ying Wang Zuobin |
| author_facet | Cui Haobo Xu Hongmei Li Jingxin Zhang Ziyu Liu Xuwei Yuan Lixin Wang Ying Wang Zuobin |
| author_sort | Cui Haobo |
| collection | DOAJ |
| description | Magnetic nanoparticles (MNPs) are often used as drug-carrying particles for targeted therapy of tumors. Studying their effects on cell activity and mechanical properties is of great significance for the targeted treatment of tumors. In this paper, we used a combination of atomic force microscopy (AFM) and fluorescent labeling to study the mechanical properties of cells after the endocytosis of MNPs. Colon cancer cells SW480 were selected to co-culture with MNPs with a particle size of 50 nm, and the cell viability was measured and systematically analyzed under different conditions. The results showed that the safe dose of MNPs to colon cancer cells SW480 was 50 µg/mL, and when the amount exceeded 50 µg/mL, the cell viability decreased significantly. Increase the concentration of MNPs step by step within the safe dose of 0-50 µg/mL. Through the analysis of a large number of data measured by AFM, the results show that the mechanical properties of cells change significantly with the increase of MNPs concentration. In this paper, the experimental results are analyzed by comparing concentration gradients. The concentrations are set to 0, 30, and 50 µg/mL , respectively, to verify the influence of MNPs on the mechanical properties of cells. |
| first_indexed | 2024-03-13T06:31:38Z |
| format | Article |
| id | doaj.art-2535bee94c9141418b59f00c88db7513 |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-03-13T06:31:38Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-2535bee94c9141418b59f00c88db75132023-06-09T08:44:26ZengEDP SciencesBIO Web of Conferences2117-44582023-01-01600100710.1051/bioconf/20236001007bioconf_bfs2023_01007Quantitative Study of the Effect of Nanoparticles on the Mechanical Properties of Colon Cancer CellsCui Haobo0Xu Hongmei1Li Jingxin2Zhang Ziyu3Liu Xuwei4Yuan Lixin5Wang Ying6Wang Zuobin7School of Optoelectronic Engineering, Changchun University of Science and TechnologySchool of Optoelectronic Engineering, Changchun University of Science and TechnologySchool of Optoelectronic Engineering, Changchun University of Science and TechnologySchool of Optoelectronic Engineering, Changchun University of Science and TechnologySchool of Optoelectronic Engineering, Changchun University of Science and TechnologySchool of Optoelectronic Engineering, Changchun University of Science and TechnologySchool of Optoelectronic Engineering, Changchun University of Science and TechnologySchool of Optoelectronic Engineering, Changchun University of Science and TechnologyMagnetic nanoparticles (MNPs) are often used as drug-carrying particles for targeted therapy of tumors. Studying their effects on cell activity and mechanical properties is of great significance for the targeted treatment of tumors. In this paper, we used a combination of atomic force microscopy (AFM) and fluorescent labeling to study the mechanical properties of cells after the endocytosis of MNPs. Colon cancer cells SW480 were selected to co-culture with MNPs with a particle size of 50 nm, and the cell viability was measured and systematically analyzed under different conditions. The results showed that the safe dose of MNPs to colon cancer cells SW480 was 50 µg/mL, and when the amount exceeded 50 µg/mL, the cell viability decreased significantly. Increase the concentration of MNPs step by step within the safe dose of 0-50 µg/mL. Through the analysis of a large number of data measured by AFM, the results show that the mechanical properties of cells change significantly with the increase of MNPs concentration. In this paper, the experimental results are analyzed by comparing concentration gradients. The concentrations are set to 0, 30, and 50 µg/mL , respectively, to verify the influence of MNPs on the mechanical properties of cells.https://www.bio-conferences.org/articles/bioconf/pdf/2023/05/bioconf_bfs2023_01007.pdf |
| spellingShingle | Cui Haobo Xu Hongmei Li Jingxin Zhang Ziyu Liu Xuwei Yuan Lixin Wang Ying Wang Zuobin Quantitative Study of the Effect of Nanoparticles on the Mechanical Properties of Colon Cancer Cells BIO Web of Conferences |
| title | Quantitative Study of the Effect of Nanoparticles on the Mechanical Properties of Colon Cancer Cells |
| title_full | Quantitative Study of the Effect of Nanoparticles on the Mechanical Properties of Colon Cancer Cells |
| title_fullStr | Quantitative Study of the Effect of Nanoparticles on the Mechanical Properties of Colon Cancer Cells |
| title_full_unstemmed | Quantitative Study of the Effect of Nanoparticles on the Mechanical Properties of Colon Cancer Cells |
| title_short | Quantitative Study of the Effect of Nanoparticles on the Mechanical Properties of Colon Cancer Cells |
| title_sort | quantitative study of the effect of nanoparticles on the mechanical properties of colon cancer cells |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2023/05/bioconf_bfs2023_01007.pdf |
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