Fluctuation of Information Entropy Measures in Cell Image
A simple, label-free cytometry technique is introduced. It is based on the analysis of the fluctuation of image Gray Level Information Entropy (GLIE) which is shown to reflect intracellular biophysical properties like generalized entropy. In this study, the analytical relations between cellular ther...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-10-01
|
| Series: | Entropy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1099-4300/19/10/565 |
| _version_ | 1811183907457990656 |
|---|---|
| author | Ishay Wohl Naomi Zurgil Yaron Hakuk Maria Sobolev Mordechai Deutsch |
| author_facet | Ishay Wohl Naomi Zurgil Yaron Hakuk Maria Sobolev Mordechai Deutsch |
| author_sort | Ishay Wohl |
| collection | DOAJ |
| description | A simple, label-free cytometry technique is introduced. It is based on the analysis of the fluctuation of image Gray Level Information Entropy (GLIE) which is shown to reflect intracellular biophysical properties like generalized entropy. In this study, the analytical relations between cellular thermodynamic generalized entropy and diffusivity and GLIE fluctuation measures are explored for the first time. The standard deviation (SD) of GLIE is shown by experiments, simulation and theoretical analysis to be indifferent to microscope system “noise”. Then, the ability of GLIE fluctuation measures to reflect basic cellular entropy conditions of early death and malignancy is demonstrated in a cell model of human, healthy-donor lymphocytes, malignant Jurkat cells, as well as dead lymphocytes and Jurkat cells. Utilization of GLIE-based fluctuation measures seems to have the advantage of displaying biophysical characterization of the tested cells, like diffusivity and entropy, in a novel, unique, simple and illustrative way. |
| first_indexed | 2024-04-11T13:04:43Z |
| format | Article |
| id | doaj.art-25364bb67e134da1b0afff839430967f |
| institution | Directory Open Access Journal |
| issn | 1099-4300 |
| language | English |
| last_indexed | 2024-04-11T13:04:43Z |
| publishDate | 2017-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Entropy |
| spelling | doaj.art-25364bb67e134da1b0afff839430967f2022-12-22T04:22:47ZengMDPI AGEntropy1099-43002017-10-01191056510.3390/e19100565e19100565Fluctuation of Information Entropy Measures in Cell ImageIshay Wohl0Naomi Zurgil1Yaron Hakuk2Maria Sobolev3Mordechai Deutsch4The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Physics Department, Bar Ilan University, Ramat-Gan 5290002, IsraelThe Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Physics Department, Bar Ilan University, Ramat-Gan 5290002, IsraelThe Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Physics Department, Bar Ilan University, Ramat-Gan 5290002, IsraelThe Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Physics Department, Bar Ilan University, Ramat-Gan 5290002, IsraelThe Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome, Physics Department, Bar Ilan University, Ramat-Gan 5290002, IsraelA simple, label-free cytometry technique is introduced. It is based on the analysis of the fluctuation of image Gray Level Information Entropy (GLIE) which is shown to reflect intracellular biophysical properties like generalized entropy. In this study, the analytical relations between cellular thermodynamic generalized entropy and diffusivity and GLIE fluctuation measures are explored for the first time. The standard deviation (SD) of GLIE is shown by experiments, simulation and theoretical analysis to be indifferent to microscope system “noise”. Then, the ability of GLIE fluctuation measures to reflect basic cellular entropy conditions of early death and malignancy is demonstrated in a cell model of human, healthy-donor lymphocytes, malignant Jurkat cells, as well as dead lymphocytes and Jurkat cells. Utilization of GLIE-based fluctuation measures seems to have the advantage of displaying biophysical characterization of the tested cells, like diffusivity and entropy, in a novel, unique, simple and illustrative way.https://www.mdpi.com/1099-4300/19/10/565image analysisinformation entropylabel-freecytometrycellular entropycellular malignancy |
| spellingShingle | Ishay Wohl Naomi Zurgil Yaron Hakuk Maria Sobolev Mordechai Deutsch Fluctuation of Information Entropy Measures in Cell Image Entropy image analysis information entropy label-free cytometry cellular entropy cellular malignancy |
| title | Fluctuation of Information Entropy Measures in Cell Image |
| title_full | Fluctuation of Information Entropy Measures in Cell Image |
| title_fullStr | Fluctuation of Information Entropy Measures in Cell Image |
| title_full_unstemmed | Fluctuation of Information Entropy Measures in Cell Image |
| title_short | Fluctuation of Information Entropy Measures in Cell Image |
| title_sort | fluctuation of information entropy measures in cell image |
| topic | image analysis information entropy label-free cytometry cellular entropy cellular malignancy |
| url | https://www.mdpi.com/1099-4300/19/10/565 |
| work_keys_str_mv | AT ishaywohl fluctuationofinformationentropymeasuresincellimage AT naomizurgil fluctuationofinformationentropymeasuresincellimage AT yaronhakuk fluctuationofinformationentropymeasuresincellimage AT mariasobolev fluctuationofinformationentropymeasuresincellimage AT mordechaideutsch fluctuationofinformationentropymeasuresincellimage |