Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro
It is a widely known that heat stress induces a reduction in milk production in cows and impairs their overall health. Studies have shown that taurine protects tissues and organs under heat stress. However, there have yet to be studies showing the functions of taurine in mammary alveolar cells-large...
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MDPI AG
2021-01-01
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| author | Hui Bai Tingting Li Yan Yu Ningcong Zhou Huijuan Kou Yingying Guo Liang Yang Peishi Yan |
| author_facet | Hui Bai Tingting Li Yan Yu Ningcong Zhou Huijuan Kou Yingying Guo Liang Yang Peishi Yan |
| author_sort | Hui Bai |
| collection | DOAJ |
| description | It is a widely known that heat stress induces a reduction in milk production in cows and impairs their overall health. Studies have shown that taurine protects tissues and organs under heat stress. However, there have yet to be studies showing the functions of taurine in mammary alveolar cells-large T antigen (MAC-T) (a bovine mammary epithelial cell line) cells under heat shock. Therefore, different concentrations of taurine (10 mM, 50 mM, and 100 mM) were tested to determine the effects on heat-induced MAC-T cells. The results showed that taurine protected the cells against heat-induced damage as shown by morphological observations in conjunction with suppressed the translocation and expression of heat shock factor 1 (HSF1). Moreover, taurine not only reversed the decline in antioxidase (superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX)) activities but also attenuated the accumulation of malondialdehyde (MDA). Meanwhile, mitochondrial damage (morphology and complex I activity) resulting from heat exposure was mitigated. Taurine also alleviated the rates of cell apoptosis and markedly depressed the mRNA expressions of BCL2 associated X, apoptosis regulator (BAX) and caspase3. Furthermore, compared with the heat stress (HS) group, the protein levels of caspase3 and cleaved caspase3 were decreased in all taurine groups. In summary, taurine improves the antioxidant and anti-apoptosis ability of MAC-T cells thereby alleviates damage of cells due to heat insults. |
| first_indexed | 2024-03-09T03:24:12Z |
| format | Article |
| id | doaj.art-25392ea117584b999b6ad6ac68d3047f |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T03:24:12Z |
| publishDate | 2021-01-01 |
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| series | Cells |
| spelling | doaj.art-25392ea117584b999b6ad6ac68d3047f2023-12-03T15:03:44ZengMDPI AGCells2073-44092021-01-0110225810.3390/cells10020258Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In VitroHui Bai0Tingting Li1Yan Yu2Ningcong Zhou3Huijuan Kou4Yingying Guo5Liang Yang6Peishi Yan7College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaUlanqab Animal Husbandry Workstation, Ulanqab 012000, ChinaUlanqab Animal Husbandry Workstation, Ulanqab 012000, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaIt is a widely known that heat stress induces a reduction in milk production in cows and impairs their overall health. Studies have shown that taurine protects tissues and organs under heat stress. However, there have yet to be studies showing the functions of taurine in mammary alveolar cells-large T antigen (MAC-T) (a bovine mammary epithelial cell line) cells under heat shock. Therefore, different concentrations of taurine (10 mM, 50 mM, and 100 mM) were tested to determine the effects on heat-induced MAC-T cells. The results showed that taurine protected the cells against heat-induced damage as shown by morphological observations in conjunction with suppressed the translocation and expression of heat shock factor 1 (HSF1). Moreover, taurine not only reversed the decline in antioxidase (superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX)) activities but also attenuated the accumulation of malondialdehyde (MDA). Meanwhile, mitochondrial damage (morphology and complex I activity) resulting from heat exposure was mitigated. Taurine also alleviated the rates of cell apoptosis and markedly depressed the mRNA expressions of BCL2 associated X, apoptosis regulator (BAX) and caspase3. Furthermore, compared with the heat stress (HS) group, the protein levels of caspase3 and cleaved caspase3 were decreased in all taurine groups. In summary, taurine improves the antioxidant and anti-apoptosis ability of MAC-T cells thereby alleviates damage of cells due to heat insults.https://www.mdpi.com/2073-4409/10/2/258MAC-T cellstaurineheat stressmitochondrial damageoxidative stresscell apoptosis |
| spellingShingle | Hui Bai Tingting Li Yan Yu Ningcong Zhou Huijuan Kou Yingying Guo Liang Yang Peishi Yan Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro Cells MAC-T cells taurine heat stress mitochondrial damage oxidative stress cell apoptosis |
| title | Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro |
| title_full | Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro |
| title_fullStr | Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro |
| title_full_unstemmed | Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro |
| title_short | Cytoprotective Effects of Taurine on Heat-Induced Bovine Mammary Epithelial Cells In Vitro |
| title_sort | cytoprotective effects of taurine on heat induced bovine mammary epithelial cells in vitro |
| topic | MAC-T cells taurine heat stress mitochondrial damage oxidative stress cell apoptosis |
| url | https://www.mdpi.com/2073-4409/10/2/258 |
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