T-cell Responses in Individuals Infected with Zika Virus and in Those Vaccinated Against Dengue Virus
Background: The outbreak of Zika virus (ZIKV) infection in Brazil has raised concerns that infection during pregnancy could cause microcephaly and other severe neurodevelopmental malformations in the fetus. The mechanisms by which ZIKV causes fetal abnormalities are largely unknown. The importance o...
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| Format: | Article |
| Language: | English |
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Case Western Reserve University
2017-06-01
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| Series: | Pathogens and Immunity |
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| Online Access: | http://paijournal.com/index.php/paijournal/article/view/188 |
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| author | Dominic Paquin-Proulx Fabio E. Leal Cassia G. Terrassani Silveira Alvino Maestri Claudia Brockmeyer Shannon M. Kitchen Vinicius D. Cabido Esper G. Kallas Douglas F. Nixon |
| author_facet | Dominic Paquin-Proulx Fabio E. Leal Cassia G. Terrassani Silveira Alvino Maestri Claudia Brockmeyer Shannon M. Kitchen Vinicius D. Cabido Esper G. Kallas Douglas F. Nixon |
| author_sort | Dominic Paquin-Proulx |
| collection | DOAJ |
| description | Background: The outbreak of Zika virus (ZIKV) infection in Brazil has raised concerns that infection during pregnancy could cause microcephaly and other severe neurodevelopmental malformations in the fetus. The mechanisms by which ZIKV causes fetal abnormalities are largely unknown. The importance of pre-infection with dengue virus (DENV), or other flaviviruses endemic to Brazil, remains to be investigated. It has been reported that antibodies directed against DENV can increase ZIKV infectivity by antibody dependent enhancement (ADE), suggesting that a history of prior DENV infection might worsen the outcome of ZIKV infection.
Methods: We used bioinformatics tools to design 18 peptides from the ZIKV envelope containing predicted HLA-I T-cell epitopes and investigated T-cell cross-reactivity between ZIKV-infected individuals and DENV-vaccinated subjects by IFNg ELISPOT.
Results: Three peptides induced IFNg production in both ZIKV-infected subjects and in DENV-vaccinated individuals. Flow cytometry indicated that 1 ZIKV peptide induced a CD4+ T-cell response in DENV-vaccinated subjects.
Conclusions: We demonstrated that vaccination against DENV induced a T-cell response against ZIKV and identified one such CD4+ T-cell epitope. The ZIKV-reactive CD4+ T cells induced by DENV vaccination and identified in this study could contribute to the appearance of cross-reactive antibodies mediating ADE. |
| first_indexed | 2024-12-10T23:30:40Z |
| format | Article |
| id | doaj.art-253d0c54abe046dfae06f24c3e2ff2ed |
| institution | Directory Open Access Journal |
| issn | 2469-2964 |
| language | English |
| last_indexed | 2024-12-10T23:30:40Z |
| publishDate | 2017-06-01 |
| publisher | Case Western Reserve University |
| record_format | Article |
| series | Pathogens and Immunity |
| spelling | doaj.art-253d0c54abe046dfae06f24c3e2ff2ed2022-12-22T01:29:24ZengCase Western Reserve UniversityPathogens and Immunity2469-29642017-06-012227429210.20411/pai.v2i2.18862T-cell Responses in Individuals Infected with Zika Virus and in Those Vaccinated Against Dengue VirusDominic Paquin-Proulx0Fabio E. Leal1Cassia G. Terrassani Silveira2Alvino Maestri3Claudia Brockmeyer4Shannon M. Kitchen5Vinicius D. Cabido6Esper G. Kallas7Douglas F. Nixon8Department of Microbiology, Immunology & Tropical Medicine, The George Washington University, Washington, D.C.Department of Microbiology, Immunology & Tropical Medicine, The George Washington University, Washington, D.C.Division of Clinical Immunology and Allergy, School of Medicine, University of São Paulo, São Paulo, SP, BrazilDivision of Clinical Immunology and Allergy, School of Medicine, University of São Paulo, São Paulo, SP, BrazilDepartment of Microbiology, Immunology & Tropical Medicine, The George Washington University, Washington, D.C.Department of Microbiology, Immunology & Tropical Medicine, The George Washington University, Washington, D.C.Department of Microbiology, Immunology & Tropical Medicine, The George Washington University, Washington, D.C.Division of Clinical Immunology and Allergy, School of Medicine, University of São Paulo, São Paulo, SP, BrazilDepartment of Microbiology, Immunology & Tropical Medicine, The George Washington University, Washington, D.C.Background: The outbreak of Zika virus (ZIKV) infection in Brazil has raised concerns that infection during pregnancy could cause microcephaly and other severe neurodevelopmental malformations in the fetus. The mechanisms by which ZIKV causes fetal abnormalities are largely unknown. The importance of pre-infection with dengue virus (DENV), or other flaviviruses endemic to Brazil, remains to be investigated. It has been reported that antibodies directed against DENV can increase ZIKV infectivity by antibody dependent enhancement (ADE), suggesting that a history of prior DENV infection might worsen the outcome of ZIKV infection. Methods: We used bioinformatics tools to design 18 peptides from the ZIKV envelope containing predicted HLA-I T-cell epitopes and investigated T-cell cross-reactivity between ZIKV-infected individuals and DENV-vaccinated subjects by IFNg ELISPOT. Results: Three peptides induced IFNg production in both ZIKV-infected subjects and in DENV-vaccinated individuals. Flow cytometry indicated that 1 ZIKV peptide induced a CD4+ T-cell response in DENV-vaccinated subjects. Conclusions: We demonstrated that vaccination against DENV induced a T-cell response against ZIKV and identified one such CD4+ T-cell epitope. The ZIKV-reactive CD4+ T cells induced by DENV vaccination and identified in this study could contribute to the appearance of cross-reactive antibodies mediating ADE.http://paijournal.com/index.php/paijournal/article/view/188zika virusdengue virusT cellIFNgELISPOTCD4antibody dependent enhancement |
| spellingShingle | Dominic Paquin-Proulx Fabio E. Leal Cassia G. Terrassani Silveira Alvino Maestri Claudia Brockmeyer Shannon M. Kitchen Vinicius D. Cabido Esper G. Kallas Douglas F. Nixon T-cell Responses in Individuals Infected with Zika Virus and in Those Vaccinated Against Dengue Virus Pathogens and Immunity zika virus dengue virus T cell IFNg ELISPOT CD4 antibody dependent enhancement |
| title | T-cell Responses in Individuals Infected with Zika Virus and in Those Vaccinated Against Dengue Virus |
| title_full | T-cell Responses in Individuals Infected with Zika Virus and in Those Vaccinated Against Dengue Virus |
| title_fullStr | T-cell Responses in Individuals Infected with Zika Virus and in Those Vaccinated Against Dengue Virus |
| title_full_unstemmed | T-cell Responses in Individuals Infected with Zika Virus and in Those Vaccinated Against Dengue Virus |
| title_short | T-cell Responses in Individuals Infected with Zika Virus and in Those Vaccinated Against Dengue Virus |
| title_sort | t cell responses in individuals infected with zika virus and in those vaccinated against dengue virus |
| topic | zika virus dengue virus T cell IFNg ELISPOT CD4 antibody dependent enhancement |
| url | http://paijournal.com/index.php/paijournal/article/view/188 |
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