The Impact of cDNA Normalization on Long-Read Sequencing of a Complex Transcriptome
Normalization of cDNA is widely used to improve the coverage of rare transcripts in analysis of transcriptomes employing next-generation sequencing. Recently, long-read technology has been emerging as a powerful tool for sequencing and construction of transcriptomes, especially for complex genomes c...
Main Authors: | Nam V. Hoang, Agnelo Furtado, Virginie Perlo, Frederik C. Botha, Robert J. Henry |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2019-07-01
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Series: | Frontiers in Genetics |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2019.00654/full |
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