The secondary spiral lamina and its relevance in cochlear implant surgery
Objective: We used synchrotron radiation phase contrast imaging (SR-PCI) to study the 3D microanatomy of the basilar membrane (BM) and its attachment to the spiral ligament (SL) (with a conceivable secondary spiral lamina [SSL] or secondary spiral plate) at the round window membrane (RWM) in the hum...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Upsala Medical Society
2018-01-01
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| Series: | Upsala Journal of Medical Sciences |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/03009734.2018.1443983 |
| _version_ | 1797707642651541504 |
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| author | Sumit Agrawal Nadine Schart-Morén Wei Liu Hanif M. Ladak Helge Rask-Andersen Hao Li |
| author_facet | Sumit Agrawal Nadine Schart-Morén Wei Liu Hanif M. Ladak Helge Rask-Andersen Hao Li |
| author_sort | Sumit Agrawal |
| collection | DOAJ |
| description | Objective: We used synchrotron radiation phase contrast imaging (SR-PCI) to study the 3D microanatomy of the basilar membrane (BM) and its attachment to the spiral ligament (SL) (with a conceivable secondary spiral lamina [SSL] or secondary spiral plate) at the round window membrane (RWM) in the human cochlea. The conception of this complex anatomy may be essential for accomplishing structural preservation at cochlear implant surgery. Material and methods: Sixteen freshly fixed human temporal bones were used to reproduce the BM, SL, primary and secondary osseous spiral laminae (OSL), and RWM using volume-rendering software. Confocal microscopy immunohistochemistry (IHC) was performed to analyze the molecular constituents. Results: SR-PCI reproduced the soft tissues including the RWM, Reissner’s membrane (RM), and the BM attachment to the lateral wall (LW) in three dimensions. A variable SR-PCI contrast enhancement was recognized in the caudal part of the SL facing the scala tympani (ST). It seemed to represent a SSL allied to the basilar crest (BC). The SSL extended along the postero-superior margin of the round window (RW) and immunohistochemically expressed type II collagen. Conclusions: Unlike in several mammalian species, the human SSL is restricted to the most basal portion of the cochlea around the RW. It anchors the BM and may influence its hydro-mechanical properties. It could also help to shield the BM from the RW. The microanatomy should be considered at cochlear implant surgery. |
| first_indexed | 2024-03-12T06:10:24Z |
| format | Article |
| id | doaj.art-253fafd2fecf4b87bc63ea75596e1af5 |
| institution | Directory Open Access Journal |
| issn | 0300-9734 2000-1967 |
| language | English |
| last_indexed | 2024-03-12T06:10:24Z |
| publishDate | 2018-01-01 |
| publisher | Upsala Medical Society |
| record_format | Article |
| series | Upsala Journal of Medical Sciences |
| spelling | doaj.art-253fafd2fecf4b87bc63ea75596e1af52023-09-03T03:05:14ZengUpsala Medical SocietyUpsala Journal of Medical Sciences0300-97342000-19672018-01-01123191810.1080/03009734.2018.14439831443983The secondary spiral lamina and its relevance in cochlear implant surgerySumit Agrawal0Nadine Schart-Morén1Wei Liu2Hanif M. Ladak3Helge Rask-Andersen4Hao Li5Western UniversityUppsala University HospitalUppsala University HospitalWestern UniversityUppsala University HospitalUppsala University HospitalObjective: We used synchrotron radiation phase contrast imaging (SR-PCI) to study the 3D microanatomy of the basilar membrane (BM) and its attachment to the spiral ligament (SL) (with a conceivable secondary spiral lamina [SSL] or secondary spiral plate) at the round window membrane (RWM) in the human cochlea. The conception of this complex anatomy may be essential for accomplishing structural preservation at cochlear implant surgery. Material and methods: Sixteen freshly fixed human temporal bones were used to reproduce the BM, SL, primary and secondary osseous spiral laminae (OSL), and RWM using volume-rendering software. Confocal microscopy immunohistochemistry (IHC) was performed to analyze the molecular constituents. Results: SR-PCI reproduced the soft tissues including the RWM, Reissner’s membrane (RM), and the BM attachment to the lateral wall (LW) in three dimensions. A variable SR-PCI contrast enhancement was recognized in the caudal part of the SL facing the scala tympani (ST). It seemed to represent a SSL allied to the basilar crest (BC). The SSL extended along the postero-superior margin of the round window (RW) and immunohistochemically expressed type II collagen. Conclusions: Unlike in several mammalian species, the human SSL is restricted to the most basal portion of the cochlea around the RW. It anchors the BM and may influence its hydro-mechanical properties. It could also help to shield the BM from the RW. The microanatomy should be considered at cochlear implant surgery.http://dx.doi.org/10.1080/03009734.2018.1443983Basilar membranecochleahumansecondary spiral laminasynchrotron-phase contrast imaging |
| spellingShingle | Sumit Agrawal Nadine Schart-Morén Wei Liu Hanif M. Ladak Helge Rask-Andersen Hao Li The secondary spiral lamina and its relevance in cochlear implant surgery Upsala Journal of Medical Sciences Basilar membrane cochlea human secondary spiral lamina synchrotron-phase contrast imaging |
| title | The secondary spiral lamina and its relevance in cochlear implant surgery |
| title_full | The secondary spiral lamina and its relevance in cochlear implant surgery |
| title_fullStr | The secondary spiral lamina and its relevance in cochlear implant surgery |
| title_full_unstemmed | The secondary spiral lamina and its relevance in cochlear implant surgery |
| title_short | The secondary spiral lamina and its relevance in cochlear implant surgery |
| title_sort | secondary spiral lamina and its relevance in cochlear implant surgery |
| topic | Basilar membrane cochlea human secondary spiral lamina synchrotron-phase contrast imaging |
| url | http://dx.doi.org/10.1080/03009734.2018.1443983 |
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