Red-Brown Pigmentation of <i>Acidipropionibacterium jensenii</i> Is Tied to Haemolytic Activity and <i>cyl-</i>Like Gene Cluster
The novel <i>Acidipropionibacterium</i> genus encompasses species of industrial importance but also those associated with food spoilage. In particular, <i>Acidipropionibacterium acidipropionici</i>, <i>Acidipropionibacterium thoenii</i>, and <i>Acidipropioni...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-10-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/7/11/512 |
| _version_ | 1818938476574277632 |
|---|---|
| author | Paulina Deptula Iida Loivamaa Olli-Pekka Smolander Pia Laine Richard J. Roberts Vieno Piironen Lars Paulin Kirsi Savijoki Petri Auvinen Pekka Varmanen |
| author_facet | Paulina Deptula Iida Loivamaa Olli-Pekka Smolander Pia Laine Richard J. Roberts Vieno Piironen Lars Paulin Kirsi Savijoki Petri Auvinen Pekka Varmanen |
| author_sort | Paulina Deptula |
| collection | DOAJ |
| description | The novel <i>Acidipropionibacterium</i> genus encompasses species of industrial importance but also those associated with food spoilage. In particular, <i>Acidipropionibacterium acidipropionici</i>, <i>Acidipropionibacterium thoenii</i>, and <i>Acidipropionibacterium jensenii</i> play an important role in food fermentation, as biopreservatives, or as potential probiotics. Notably, <i>A. jensenii</i> and <i>A. thoenii</i> can cause brown spot defects in Swiss-type cheeses, which have been tied to the rhamnolipid pigment granadaene. In the pathogenic bacterium <i>Streptococcus agalactiae,</i> production of granadaene depends on the presence of a <i>cyl</i> gene cluster, an important virulence factor linked with haemolytic activity. Here, we show that the production of granadaene in pigmented <i>Acidipropionibacterium,</i> including <i>A. jensenii, A. thoenii,</i> and <i>Acidipropionibacterium virtanenii</i>, is tied to haemolytic activity and the presence of a <i>cyl</i>-like gene cluster. Furthermore, we propose a PCR-based test, which allows pinpointing acidipropionibacteria with the <i>cyl</i>-like gene cluster. Finally, we present the first two whole genome sequence analyses of the <i>A. jensenii</i> strains as well as testing phenotypic characteristics important for industrial applications. In conclusion, the present study sheds light on potential risks associated with the presence of pigmented <i>Acidipropionibacterium</i> strains in food fermentation. In addition, the results presented here provide ground for development of a quick and simple diagnostic test instrumental in avoiding potential negative effects of <i>Acidipropionibacterium</i> strains with haemolytic activity on food quality. |
| first_indexed | 2024-12-20T06:08:28Z |
| format | Article |
| id | doaj.art-254a1dfd67954f6d8180aecf2f247a67 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-20T06:08:28Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-254a1dfd67954f6d8180aecf2f247a672022-12-21T19:50:45ZengMDPI AGMicroorganisms2076-26072019-10-0171151210.3390/microorganisms7110512microorganisms7110512Red-Brown Pigmentation of <i>Acidipropionibacterium jensenii</i> Is Tied to Haemolytic Activity and <i>cyl-</i>Like Gene ClusterPaulina Deptula0Iida Loivamaa1Olli-Pekka Smolander2Pia Laine3Richard J. Roberts4Vieno Piironen5Lars Paulin6Kirsi Savijoki7Petri Auvinen8Pekka Varmanen9Institute of Biotechnology, University of Helsinki, 00014 Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, 00014 Helsinki, FinlandInstitute of Biotechnology, University of Helsinki, 00014 Helsinki, FinlandInstitute of Biotechnology, University of Helsinki, 00014 Helsinki, FinlandNew England Biolabs, Ipswich, MA 01938-2723, USADepartment of Food and Nutrition, University of Helsinki, 00014 Helsinki, FinlandInstitute of Biotechnology, University of Helsinki, 00014 Helsinki, FinlandDivision of Pharmaceutical Biosciences, University of Helsinki, 00014 Helsinki, FinlandInstitute of Biotechnology, University of Helsinki, 00014 Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, 00014 Helsinki, FinlandThe novel <i>Acidipropionibacterium</i> genus encompasses species of industrial importance but also those associated with food spoilage. In particular, <i>Acidipropionibacterium acidipropionici</i>, <i>Acidipropionibacterium thoenii</i>, and <i>Acidipropionibacterium jensenii</i> play an important role in food fermentation, as biopreservatives, or as potential probiotics. Notably, <i>A. jensenii</i> and <i>A. thoenii</i> can cause brown spot defects in Swiss-type cheeses, which have been tied to the rhamnolipid pigment granadaene. In the pathogenic bacterium <i>Streptococcus agalactiae,</i> production of granadaene depends on the presence of a <i>cyl</i> gene cluster, an important virulence factor linked with haemolytic activity. Here, we show that the production of granadaene in pigmented <i>Acidipropionibacterium,</i> including <i>A. jensenii, A. thoenii,</i> and <i>Acidipropionibacterium virtanenii</i>, is tied to haemolytic activity and the presence of a <i>cyl</i>-like gene cluster. Furthermore, we propose a PCR-based test, which allows pinpointing acidipropionibacteria with the <i>cyl</i>-like gene cluster. Finally, we present the first two whole genome sequence analyses of the <i>A. jensenii</i> strains as well as testing phenotypic characteristics important for industrial applications. In conclusion, the present study sheds light on potential risks associated with the presence of pigmented <i>Acidipropionibacterium</i> strains in food fermentation. In addition, the results presented here provide ground for development of a quick and simple diagnostic test instrumental in avoiding potential negative effects of <i>Acidipropionibacterium</i> strains with haemolytic activity on food quality.https://www.mdpi.com/2076-2607/7/11/512genomehaemolysisgranadaenepigmentation<i>acidipropionibacterium thoenii</i><i>acidipropionibacterium virtanenii</i> |
| spellingShingle | Paulina Deptula Iida Loivamaa Olli-Pekka Smolander Pia Laine Richard J. Roberts Vieno Piironen Lars Paulin Kirsi Savijoki Petri Auvinen Pekka Varmanen Red-Brown Pigmentation of <i>Acidipropionibacterium jensenii</i> Is Tied to Haemolytic Activity and <i>cyl-</i>Like Gene Cluster Microorganisms genome haemolysis granadaene pigmentation <i>acidipropionibacterium thoenii</i> <i>acidipropionibacterium virtanenii</i> |
| title | Red-Brown Pigmentation of <i>Acidipropionibacterium jensenii</i> Is Tied to Haemolytic Activity and <i>cyl-</i>Like Gene Cluster |
| title_full | Red-Brown Pigmentation of <i>Acidipropionibacterium jensenii</i> Is Tied to Haemolytic Activity and <i>cyl-</i>Like Gene Cluster |
| title_fullStr | Red-Brown Pigmentation of <i>Acidipropionibacterium jensenii</i> Is Tied to Haemolytic Activity and <i>cyl-</i>Like Gene Cluster |
| title_full_unstemmed | Red-Brown Pigmentation of <i>Acidipropionibacterium jensenii</i> Is Tied to Haemolytic Activity and <i>cyl-</i>Like Gene Cluster |
| title_short | Red-Brown Pigmentation of <i>Acidipropionibacterium jensenii</i> Is Tied to Haemolytic Activity and <i>cyl-</i>Like Gene Cluster |
| title_sort | red brown pigmentation of i acidipropionibacterium jensenii i is tied to haemolytic activity and i cyl i like gene cluster |
| topic | genome haemolysis granadaene pigmentation <i>acidipropionibacterium thoenii</i> <i>acidipropionibacterium virtanenii</i> |
| url | https://www.mdpi.com/2076-2607/7/11/512 |
| work_keys_str_mv | AT paulinadeptula redbrownpigmentationofiacidipropionibacteriumjenseniiiistiedtohaemolyticactivityandicylilikegenecluster AT iidaloivamaa redbrownpigmentationofiacidipropionibacteriumjenseniiiistiedtohaemolyticactivityandicylilikegenecluster AT ollipekkasmolander redbrownpigmentationofiacidipropionibacteriumjenseniiiistiedtohaemolyticactivityandicylilikegenecluster AT pialaine redbrownpigmentationofiacidipropionibacteriumjenseniiiistiedtohaemolyticactivityandicylilikegenecluster AT richardjroberts redbrownpigmentationofiacidipropionibacteriumjenseniiiistiedtohaemolyticactivityandicylilikegenecluster AT vienopiironen redbrownpigmentationofiacidipropionibacteriumjenseniiiistiedtohaemolyticactivityandicylilikegenecluster AT larspaulin redbrownpigmentationofiacidipropionibacteriumjenseniiiistiedtohaemolyticactivityandicylilikegenecluster AT kirsisavijoki redbrownpigmentationofiacidipropionibacteriumjenseniiiistiedtohaemolyticactivityandicylilikegenecluster AT petriauvinen redbrownpigmentationofiacidipropionibacteriumjenseniiiistiedtohaemolyticactivityandicylilikegenecluster AT pekkavarmanen redbrownpigmentationofiacidipropionibacteriumjenseniiiistiedtohaemolyticactivityandicylilikegenecluster |