Interaction of vascular endothelial cells with hydrophilic fullerene nanoarchitectured structures in 2D and 3D environments
ABSTRACTThe interaction between diverse nanoarchitectured fullerenes and cells is crucial for biomedical applications. Here, we detailed the preparation of hydrophilic self-assembled fullerenes by the liquid-liquid interfacial precipitation (LLIP) method and hydrophilic coating of the materials as a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Science and Technology of Advanced Materials |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/14686996.2024.2315014 |
| _version_ | 1797293770425761792 |
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| author | Tsai-Yu Chen Kun-Chih Cheng Pei-Syuan Yang Lok Kumar Shrestha Katsuhiko Ariga Shan-hui Hsu |
| author_facet | Tsai-Yu Chen Kun-Chih Cheng Pei-Syuan Yang Lok Kumar Shrestha Katsuhiko Ariga Shan-hui Hsu |
| author_sort | Tsai-Yu Chen |
| collection | DOAJ |
| description | ABSTRACTThe interaction between diverse nanoarchitectured fullerenes and cells is crucial for biomedical applications. Here, we detailed the preparation of hydrophilic self-assembled fullerenes by the liquid-liquid interfacial precipitation (LLIP) method and hydrophilic coating of the materials as a possible vascularization strategy. The interactions of vascular endothelial cells (ECs) with hydrophilic fullerene nanotubes (FNT-P) and hydrophilic fullerene nanowhiskers (FNW-P) were investigated. The average length and diameter of FNT-P were 16 ± 2 μm and 3.4 ± 0.4 μm (i.e. aspect ratios of 4.6), respectively. The average length and diameter of FNW-P were 65 ± 8 μm and 1.2 ± 0.2 μm (i.e. aspect ratios of 53.9), respectively. For two-dimensional (2D) culture after 7 days, the ECs remained viable and proliferated up to ~ 420% and ~ 400% with FNT-P and FNW-P of 50 μg/mL, respectively. Furthermore, an optimized chitosan-based self-healing hydrogel with a modulus of ~400 Pa was developed and used to incorporate self-assembled fullerenes as in vitro three-dimensional (3D) platforms to investigate the impact of FNT-P and FNW-P on ECs within a 3D environment. The addition of FNW-P or FNT-P (50 μg/mL) in the hydrogel system led to proliferation rates of ECs up to ~323% and ~280%, respectively, after 7 days of culture. The ECs in FNW-P hydrogel displayed an elongated shape with aligned morphology, while those in FNT-P hydrogel exhibited a rounded and clustered distribution. Vascular-related gene expressions of ECs were significantly upregulated through interactions with these fullerenes. Thus, the combined use of different nanoarchitectured self-assembled fullerenes and self-healing hydrogels may offer environmental cues influencing EC development in a 3D biomimetic microenvironment, holding promise for advancing vascularization strategy in tissue engineering. |
| first_indexed | 2024-03-07T21:20:40Z |
| format | Article |
| id | doaj.art-254a2e8a4b294dfb81b3130078aa524b |
| institution | Directory Open Access Journal |
| issn | 1468-6996 1878-5514 |
| language | English |
| last_indexed | 2024-03-07T21:20:40Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
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| series | Science and Technology of Advanced Materials |
| spelling | doaj.art-254a2e8a4b294dfb81b3130078aa524b2024-02-27T13:51:51ZengTaylor & Francis GroupScience and Technology of Advanced Materials1468-69961878-55142024-12-0125110.1080/14686996.2024.2315014Interaction of vascular endothelial cells with hydrophilic fullerene nanoarchitectured structures in 2D and 3D environmentsTsai-Yu Chen0Kun-Chih Cheng1Pei-Syuan Yang2Lok Kumar Shrestha3Katsuhiko Ariga4Shan-hui Hsu5Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan, R.O.CInstitute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan, R.O.CInstitute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan, R.O.CSupermolecules Group, Research Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), Tsukuba, JapanSupermolecules Group, Research Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), Tsukuba, JapanInstitute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan, R.O.CABSTRACTThe interaction between diverse nanoarchitectured fullerenes and cells is crucial for biomedical applications. Here, we detailed the preparation of hydrophilic self-assembled fullerenes by the liquid-liquid interfacial precipitation (LLIP) method and hydrophilic coating of the materials as a possible vascularization strategy. The interactions of vascular endothelial cells (ECs) with hydrophilic fullerene nanotubes (FNT-P) and hydrophilic fullerene nanowhiskers (FNW-P) were investigated. The average length and diameter of FNT-P were 16 ± 2 μm and 3.4 ± 0.4 μm (i.e. aspect ratios of 4.6), respectively. The average length and diameter of FNW-P were 65 ± 8 μm and 1.2 ± 0.2 μm (i.e. aspect ratios of 53.9), respectively. For two-dimensional (2D) culture after 7 days, the ECs remained viable and proliferated up to ~ 420% and ~ 400% with FNT-P and FNW-P of 50 μg/mL, respectively. Furthermore, an optimized chitosan-based self-healing hydrogel with a modulus of ~400 Pa was developed and used to incorporate self-assembled fullerenes as in vitro three-dimensional (3D) platforms to investigate the impact of FNT-P and FNW-P on ECs within a 3D environment. The addition of FNW-P or FNT-P (50 μg/mL) in the hydrogel system led to proliferation rates of ECs up to ~323% and ~280%, respectively, after 7 days of culture. The ECs in FNW-P hydrogel displayed an elongated shape with aligned morphology, while those in FNT-P hydrogel exhibited a rounded and clustered distribution. Vascular-related gene expressions of ECs were significantly upregulated through interactions with these fullerenes. Thus, the combined use of different nanoarchitectured self-assembled fullerenes and self-healing hydrogels may offer environmental cues influencing EC development in a 3D biomimetic microenvironment, holding promise for advancing vascularization strategy in tissue engineering.https://www.tandfonline.com/doi/10.1080/14686996.2024.2315014Self-assembled fullerenesnanotubenanowhiskerself-healing hydrogelendothelial cellsvascularization |
| spellingShingle | Tsai-Yu Chen Kun-Chih Cheng Pei-Syuan Yang Lok Kumar Shrestha Katsuhiko Ariga Shan-hui Hsu Interaction of vascular endothelial cells with hydrophilic fullerene nanoarchitectured structures in 2D and 3D environments Science and Technology of Advanced Materials Self-assembled fullerenes nanotube nanowhisker self-healing hydrogel endothelial cells vascularization |
| title | Interaction of vascular endothelial cells with hydrophilic fullerene nanoarchitectured structures in 2D and 3D environments |
| title_full | Interaction of vascular endothelial cells with hydrophilic fullerene nanoarchitectured structures in 2D and 3D environments |
| title_fullStr | Interaction of vascular endothelial cells with hydrophilic fullerene nanoarchitectured structures in 2D and 3D environments |
| title_full_unstemmed | Interaction of vascular endothelial cells with hydrophilic fullerene nanoarchitectured structures in 2D and 3D environments |
| title_short | Interaction of vascular endothelial cells with hydrophilic fullerene nanoarchitectured structures in 2D and 3D environments |
| title_sort | interaction of vascular endothelial cells with hydrophilic fullerene nanoarchitectured structures in 2d and 3d environments |
| topic | Self-assembled fullerenes nanotube nanowhisker self-healing hydrogel endothelial cells vascularization |
| url | https://www.tandfonline.com/doi/10.1080/14686996.2024.2315014 |
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