Catalase T-Deficient Fission Yeast Meiocytes Show Resistance to Ionizing Radiation
Environmental stress, reactive oxygen species (ROS), or ionizing radiation (IR) can induce adverse effects in organisms and their cells, including mutations and premature aging. DNA damage and its faulty repair can lead to cell death or promote cancer through the accumulation of mutations. Misrepair...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/9/881 |
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| author | Razan Muhtadi Alexander Lorenz Samantha J. Mpaulo Christian Siebenwirth Harry Scherthan |
| author_facet | Razan Muhtadi Alexander Lorenz Samantha J. Mpaulo Christian Siebenwirth Harry Scherthan |
| author_sort | Razan Muhtadi |
| collection | DOAJ |
| description | Environmental stress, reactive oxygen species (ROS), or ionizing radiation (IR) can induce adverse effects in organisms and their cells, including mutations and premature aging. DNA damage and its faulty repair can lead to cell death or promote cancer through the accumulation of mutations. Misrepair in germ cells is particularly dangerous as it may lead to alterations in developmental programs and genetic disease in the offspring. DNA damage pathways and radical defense mechanisms mediate resistance to genotoxic stresses. Here, we investigated, in the fission yeast <i>Schizosaccharomyces pombe</i>, the role of the H<sub>2</sub>O<sub>2</sub>-detoxifying enzyme cytosolic catalase T (Ctt1) and the Fe<sup>2+</sup>/Mn<sup>2+</sup> symporter Pcl1 in protecting meiotic chromosome dynamics and gamete formation from radicals generated by ROS and IR. We found that wild-type and <i>pcl1</i>-deficient cells respond similarly to X ray doses of up to 300 Gy, while <i>ctt1</i>∆ meiocytes showed a moderate sensitivity to IR but a hypersensitivity to hydrogen peroxide with cells dying at >0.4 mM H<sub>2</sub>O<sub>2</sub>. Meiocytes deficient for <i>pcl1</i>, on the other hand, showed a resistance to hydrogen peroxide similar to that of the wild type, surviving doses >40 mM. In all, it appears that in the absence of the main H<sub>2</sub>O<sub>2</sub>-detoxifying pathway <i>S. pombe</i> meiocytes are able to survive significant doses of IR-induced radicals. |
| first_indexed | 2024-03-10T16:15:21Z |
| format | Article |
| id | doaj.art-254a7a7553744b3fbad55fe10fc95fb3 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T16:15:21Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-254a7a7553744b3fbad55fe10fc95fb32023-11-20T14:05:40ZengMDPI AGAntioxidants2076-39212020-09-019988110.3390/antiox9090881Catalase T-Deficient Fission Yeast Meiocytes Show Resistance to Ionizing RadiationRazan Muhtadi0Alexander Lorenz1Samantha J. Mpaulo2Christian Siebenwirth3Harry Scherthan4Institut für Radiobiologie der Bundeswehr in Verb. mit der Universität Ulm, Neuherbergstr. 11, D-80937 Munich, GermanyInstitute of Medical Sciences (IMS), University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UKInstitute of Medical Sciences (IMS), University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UKInstitut für Radiobiologie der Bundeswehr in Verb. mit der Universität Ulm, Neuherbergstr. 11, D-80937 Munich, GermanyInstitut für Radiobiologie der Bundeswehr in Verb. mit der Universität Ulm, Neuherbergstr. 11, D-80937 Munich, GermanyEnvironmental stress, reactive oxygen species (ROS), or ionizing radiation (IR) can induce adverse effects in organisms and their cells, including mutations and premature aging. DNA damage and its faulty repair can lead to cell death or promote cancer through the accumulation of mutations. Misrepair in germ cells is particularly dangerous as it may lead to alterations in developmental programs and genetic disease in the offspring. DNA damage pathways and radical defense mechanisms mediate resistance to genotoxic stresses. Here, we investigated, in the fission yeast <i>Schizosaccharomyces pombe</i>, the role of the H<sub>2</sub>O<sub>2</sub>-detoxifying enzyme cytosolic catalase T (Ctt1) and the Fe<sup>2+</sup>/Mn<sup>2+</sup> symporter Pcl1 in protecting meiotic chromosome dynamics and gamete formation from radicals generated by ROS and IR. We found that wild-type and <i>pcl1</i>-deficient cells respond similarly to X ray doses of up to 300 Gy, while <i>ctt1</i>∆ meiocytes showed a moderate sensitivity to IR but a hypersensitivity to hydrogen peroxide with cells dying at >0.4 mM H<sub>2</sub>O<sub>2</sub>. Meiocytes deficient for <i>pcl1</i>, on the other hand, showed a resistance to hydrogen peroxide similar to that of the wild type, surviving doses >40 mM. In all, it appears that in the absence of the main H<sub>2</sub>O<sub>2</sub>-detoxifying pathway <i>S. pombe</i> meiocytes are able to survive significant doses of IR-induced radicals.https://www.mdpi.com/2076-3921/9/9/881chromosome mobilityCtt1horsetail movementionizing radiationmeiosisradical stress |
| spellingShingle | Razan Muhtadi Alexander Lorenz Samantha J. Mpaulo Christian Siebenwirth Harry Scherthan Catalase T-Deficient Fission Yeast Meiocytes Show Resistance to Ionizing Radiation Antioxidants chromosome mobility Ctt1 horsetail movement ionizing radiation meiosis radical stress |
| title | Catalase T-Deficient Fission Yeast Meiocytes Show Resistance to Ionizing Radiation |
| title_full | Catalase T-Deficient Fission Yeast Meiocytes Show Resistance to Ionizing Radiation |
| title_fullStr | Catalase T-Deficient Fission Yeast Meiocytes Show Resistance to Ionizing Radiation |
| title_full_unstemmed | Catalase T-Deficient Fission Yeast Meiocytes Show Resistance to Ionizing Radiation |
| title_short | Catalase T-Deficient Fission Yeast Meiocytes Show Resistance to Ionizing Radiation |
| title_sort | catalase t deficient fission yeast meiocytes show resistance to ionizing radiation |
| topic | chromosome mobility Ctt1 horsetail movement ionizing radiation meiosis radical stress |
| url | https://www.mdpi.com/2076-3921/9/9/881 |
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