Identification and Characterization of <i>Verticillium nonalfalfae</i>-Responsive MicroRNAs in the Roots of Resistant and Susceptible Hop Cultivars
MicroRNAs are 21- to 24-nucleotide-long, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. They can modulate various biological processes, including plant response and resistance to fungal pathogens. Hops are grown for use in the brewing industry and, recently...
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MDPI AG
2021-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/9/1883 |
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| author | Urban Kunej Jernej Jakše Sebastjan Radišek Nataša Štajner |
| author_facet | Urban Kunej Jernej Jakše Sebastjan Radišek Nataša Štajner |
| author_sort | Urban Kunej |
| collection | DOAJ |
| description | MicroRNAs are 21- to 24-nucleotide-long, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. They can modulate various biological processes, including plant response and resistance to fungal pathogens. Hops are grown for use in the brewing industry and, recently, also for the pharmaceutical industry. Severe Verticillium wilt caused by the phytopathogenic fungus <i>Verticillium nonalfalfae</i>, is the main factor in yield loss in many crops, including hops (<i>Humulus lupulus</i> L.). In our study, we identified 56 known and 43 novel miRNAs and their expression patterns in the roots of susceptible and resistant hop cultivars after inoculation with <i>V. nonalfalfae</i>. In response to inoculation with <i>V. nonalfalfae</i>, we found five known and two novel miRNAs that are differentially expressed in the susceptible cultivar and six known miRNAs in the resistant cultivar. Differentially expressed miRNAs target 49 transcripts involved in protein localization and pigment synthesis in the susceptible cultivar, whereas they are involved in transcription factor regulation and hormone signalling in the resistant cultivar. The results of our study suggest that the susceptible and resistant hop cultivars respond differently to <i>V. nonalfalfae</i> inoculation at the miRNA level and that miRNAs may contribute to the successful defence of the resistant cultivar. |
| first_indexed | 2024-03-10T07:17:07Z |
| format | Article |
| id | doaj.art-254a9542f6224dae9da5e1309ac8fe29 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T07:17:07Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-254a9542f6224dae9da5e1309ac8fe292023-11-22T14:53:12ZengMDPI AGPlants2223-77472021-09-01109188310.3390/plants10091883Identification and Characterization of <i>Verticillium nonalfalfae</i>-Responsive MicroRNAs in the Roots of Resistant and Susceptible Hop CultivarsUrban Kunej0Jernej Jakše1Sebastjan Radišek2Nataša Štajner3Department of Agronomy, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaDepartment of Agronomy, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaPlant Protection Department, Slovenian Institute of Hop Research and Brewing, 3310 Žalec, SloveniaDepartment of Agronomy, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaMicroRNAs are 21- to 24-nucleotide-long, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. They can modulate various biological processes, including plant response and resistance to fungal pathogens. Hops are grown for use in the brewing industry and, recently, also for the pharmaceutical industry. Severe Verticillium wilt caused by the phytopathogenic fungus <i>Verticillium nonalfalfae</i>, is the main factor in yield loss in many crops, including hops (<i>Humulus lupulus</i> L.). In our study, we identified 56 known and 43 novel miRNAs and their expression patterns in the roots of susceptible and resistant hop cultivars after inoculation with <i>V. nonalfalfae</i>. In response to inoculation with <i>V. nonalfalfae</i>, we found five known and two novel miRNAs that are differentially expressed in the susceptible cultivar and six known miRNAs in the resistant cultivar. Differentially expressed miRNAs target 49 transcripts involved in protein localization and pigment synthesis in the susceptible cultivar, whereas they are involved in transcription factor regulation and hormone signalling in the resistant cultivar. The results of our study suggest that the susceptible and resistant hop cultivars respond differently to <i>V. nonalfalfae</i> inoculation at the miRNA level and that miRNAs may contribute to the successful defence of the resistant cultivar.https://www.mdpi.com/2223-7747/10/9/1883<i>Humulus lupulus</i><i>Verticillium nonalfalfae</i>biotic stressmicroRNAhigh-throughput sequencing |
| spellingShingle | Urban Kunej Jernej Jakše Sebastjan Radišek Nataša Štajner Identification and Characterization of <i>Verticillium nonalfalfae</i>-Responsive MicroRNAs in the Roots of Resistant and Susceptible Hop Cultivars Plants <i>Humulus lupulus</i> <i>Verticillium nonalfalfae</i> biotic stress microRNA high-throughput sequencing |
| title | Identification and Characterization of <i>Verticillium nonalfalfae</i>-Responsive MicroRNAs in the Roots of Resistant and Susceptible Hop Cultivars |
| title_full | Identification and Characterization of <i>Verticillium nonalfalfae</i>-Responsive MicroRNAs in the Roots of Resistant and Susceptible Hop Cultivars |
| title_fullStr | Identification and Characterization of <i>Verticillium nonalfalfae</i>-Responsive MicroRNAs in the Roots of Resistant and Susceptible Hop Cultivars |
| title_full_unstemmed | Identification and Characterization of <i>Verticillium nonalfalfae</i>-Responsive MicroRNAs in the Roots of Resistant and Susceptible Hop Cultivars |
| title_short | Identification and Characterization of <i>Verticillium nonalfalfae</i>-Responsive MicroRNAs in the Roots of Resistant and Susceptible Hop Cultivars |
| title_sort | identification and characterization of i verticillium nonalfalfae i responsive micrornas in the roots of resistant and susceptible hop cultivars |
| topic | <i>Humulus lupulus</i> <i>Verticillium nonalfalfae</i> biotic stress microRNA high-throughput sequencing |
| url | https://www.mdpi.com/2223-7747/10/9/1883 |
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