Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice
Abstract A nano‐enabled drug delivery acupuncture technology (nd‐Acu) is developed that is based on traditional acupuncture needles where the stainless‐steel surface is designed to deliver various payload molecules. To create the nd‐Acu platform, an electrochemistry procedure is used to attach methy...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-10-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202302586 |
| _version_ | 1797663854177550336 |
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| author | Wenjie Xu Yu Xiao Minzhi Zhao Jiahui Zhu Yu Wang Wenbin Wang Peng Wang Huan Meng |
| author_facet | Wenjie Xu Yu Xiao Minzhi Zhao Jiahui Zhu Yu Wang Wenbin Wang Peng Wang Huan Meng |
| author_sort | Wenjie Xu |
| collection | DOAJ |
| description | Abstract A nano‐enabled drug delivery acupuncture technology (nd‐Acu) is developed that is based on traditional acupuncture needles where the stainless‐steel surface is designed to deliver various payload molecules. To create the nd‐Acu platform, an electrochemistry procedure is used to attach methyl salicylate‐modified cyclodextrin in which the sugar rings allow the encapsulation of structurally defined single or multiple payload molecules via an inclusion complexation process. Drug loading and release profile are first studied using fluorescent dyes abiotically and at intact animal level. nd‐Acu allows more efficient dye loading and time‐dependent release compared to pristine needles without cyclodextrin modification. Subsequently, a proof‐of‐principle efficacy study is conducted using the platform to load a local anesthetic, lidocaine, for the treatment of knee osteoarthritis (KOA) in mice. It is demonstrated that lidocaine‐laden nd‐Acu can effectively alleviate pain, reduce inflammation, and slow down KOA development biochemically and histologically. Hypothesis‐driven and proteomic approaches are utilized to investigate the working mechanisms of lidocaine nd‐Acu, indicating that the therapeutic outcome is attributed to the in vivo modulation of the HMGB1/TLR4 signaling pathway. The study also obtained preliminary evidence suggesting the involvement of mitochondria as well as small GTPase such as cdc42 during the treatment by lidocaine nd‐Acu. |
| first_indexed | 2024-03-11T19:21:48Z |
| format | Article |
| id | doaj.art-254d1a36f4c74ec78753c4776cf9b79c |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-11T19:21:48Z |
| publishDate | 2023-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-254d1a36f4c74ec78753c4776cf9b79c2023-10-07T03:51:49ZengWileyAdvanced Science2198-38442023-10-011028n/an/a10.1002/advs.202302586Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in MiceWenjie Xu0Yu Xiao1Minzhi Zhao2Jiahui Zhu3Yu Wang4Wenbin Wang5Peng Wang6Huan Meng7CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 ChinaCAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 ChinaCAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 ChinaCAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 ChinaCAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 ChinaCAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 ChinaBeijing Hospital of Traditional Chinese Medicine Capital Medical University Beijing 100010 ChinaCAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 ChinaAbstract A nano‐enabled drug delivery acupuncture technology (nd‐Acu) is developed that is based on traditional acupuncture needles where the stainless‐steel surface is designed to deliver various payload molecules. To create the nd‐Acu platform, an electrochemistry procedure is used to attach methyl salicylate‐modified cyclodextrin in which the sugar rings allow the encapsulation of structurally defined single or multiple payload molecules via an inclusion complexation process. Drug loading and release profile are first studied using fluorescent dyes abiotically and at intact animal level. nd‐Acu allows more efficient dye loading and time‐dependent release compared to pristine needles without cyclodextrin modification. Subsequently, a proof‐of‐principle efficacy study is conducted using the platform to load a local anesthetic, lidocaine, for the treatment of knee osteoarthritis (KOA) in mice. It is demonstrated that lidocaine‐laden nd‐Acu can effectively alleviate pain, reduce inflammation, and slow down KOA development biochemically and histologically. Hypothesis‐driven and proteomic approaches are utilized to investigate the working mechanisms of lidocaine nd‐Acu, indicating that the therapeutic outcome is attributed to the in vivo modulation of the HMGB1/TLR4 signaling pathway. The study also obtained preliminary evidence suggesting the involvement of mitochondria as well as small GTPase such as cdc42 during the treatment by lidocaine nd‐Acu.https://doi.org/10.1002/advs.202302586acupunctureknee osteoarthritislidocainenano drug deliverynd‐Acu |
| spellingShingle | Wenjie Xu Yu Xiao Minzhi Zhao Jiahui Zhu Yu Wang Wenbin Wang Peng Wang Huan Meng Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice Advanced Science acupuncture knee osteoarthritis lidocaine nano drug delivery nd‐Acu |
| title | Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice |
| title_full | Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice |
| title_fullStr | Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice |
| title_full_unstemmed | Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice |
| title_short | Effective Treatment of Knee Osteoarthritis Using a Nano‐Enabled Drug Acupuncture Technology in Mice |
| title_sort | effective treatment of knee osteoarthritis using a nano enabled drug acupuncture technology in mice |
| topic | acupuncture knee osteoarthritis lidocaine nano drug delivery nd‐Acu |
| url | https://doi.org/10.1002/advs.202302586 |
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