Prevalence and Characterisation of Multiresistant Bacterial Strains Isolated in Pigs from the Island of Tenerife
Background: Antibiotic-resistant bacteria can circulate among human and animal populations through direct contact with animals, as well as via food and the environment. The purpose of this study was to examine the prevalence and characterisation of multiresistant bacteria in pig samples. Methods: 22...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/6/269 |
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| author | Rossana Abreu Cristobalina Rodríguez-Álvarez Beatriz Castro-Hernandez Maria Lecuona-Fernández Juan Carlos González Yurena Rodríguez-Novo Maria de los Angeles Arias Rodríguez |
| author_facet | Rossana Abreu Cristobalina Rodríguez-Álvarez Beatriz Castro-Hernandez Maria Lecuona-Fernández Juan Carlos González Yurena Rodríguez-Novo Maria de los Angeles Arias Rodríguez |
| author_sort | Rossana Abreu |
| collection | DOAJ |
| description | Background: Antibiotic-resistant bacteria can circulate among human and animal populations through direct contact with animals, as well as via food and the environment. The purpose of this study was to examine the prevalence and characterisation of multiresistant bacteria in pig samples. Methods: 224 samples of pig livestock were taken at the slaughterhouse on the island of Tenerife. A nasal and a rectal sample were collected from each pig. The presence of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), methicillin-resistant <i>Staphylococcus</i> coagulase-negative (MRCoNS), vancomycin-resistant Enterococcus (VRE), extended-spectrum ß-lactamase-producing <i>Enterobacteriaceae</i> (BLEE), carbapenemase-producing <i>Enterobacteriaceae</i> (CPE), and colistin-resistant Enterobacteriaceae was investigated. The resistance genes of the isolated bacteria were characterised by specific PCRs depending on the microorganism to be studied, and in vitro antimicrobial resistance was determined using the broth microdilution method (Vitek<sup>®</sup>2 system bioMérieux<sup>®</sup>, Nurtingen, Germany). Results: MRSA prevalence was 73.21% (164 isolates). MRCoNS prevalence was 9.8% (22 isolates), <i>S. sciuri</i> being the prevalent species. Six isolates presented a 2.7% prevalence of extended-spectrum ß-lactamase-producing <i>Escherichia coli</i> (BLEE) in the CTX-M-1 group. No vancomycin-resistant <i>Enterococcus</i> (VRE), carbapenemase-producing <i>Enterobacteriaceae</i> (CRE), or colistin-resistant <i>Enterobacteriaceae</i> were isolated. Conclusion: we found a high presence of multiresistant bacteria, suggesting the need for increased control and surveillance of this type of strains in pig livestock and a better understanding of the possible transmission routes of these microorganisms through livestock products. |
| first_indexed | 2024-03-09T22:16:00Z |
| format | Article |
| id | doaj.art-254d3016f4de4a768360f1d969eadbba |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T22:16:00Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-254d3016f4de4a768360f1d969eadbba2023-11-23T19:23:07ZengMDPI AGVeterinary Sciences2306-73812022-06-019626910.3390/vetsci9060269Prevalence and Characterisation of Multiresistant Bacterial Strains Isolated in Pigs from the Island of TenerifeRossana Abreu0Cristobalina Rodríguez-Álvarez1Beatriz Castro-Hernandez2Maria Lecuona-Fernández3Juan Carlos González4Yurena Rodríguez-Novo5Maria de los Angeles Arias Rodríguez6Department of Preventive Medicine and Public Health, University of La Laguna, Campus de Ofra, s/n, 38071 Santa Cruz de Tenerife, SpainDepartment of Preventive Medicine and Public Health, University of La Laguna, Campus de Ofra, s/n, 38071 Santa Cruz de Tenerife, SpainMicrobiology and Infection Control Service, University Hospital of the Canary Islands, Canary Islands, Tenerife, 38320 San Cristóbal de La Laguna, SpainMicrobiology and Infection Control Service, University Hospital of the Canary Islands, Canary Islands, Tenerife, 38320 San Cristóbal de La Laguna, SpainCanary Islands Health Service, Canary Islands, 38004 Santa Cruz de Tenerife, SpainFaculty of Health Sciences, Nursing Section, University of La Laguna, Tenerife, 38200 La Laguna, SpainDepartment of Preventive Medicine and Public Health, University of La Laguna, Campus de Ofra, s/n, 38071 Santa Cruz de Tenerife, SpainBackground: Antibiotic-resistant bacteria can circulate among human and animal populations through direct contact with animals, as well as via food and the environment. The purpose of this study was to examine the prevalence and characterisation of multiresistant bacteria in pig samples. Methods: 224 samples of pig livestock were taken at the slaughterhouse on the island of Tenerife. A nasal and a rectal sample were collected from each pig. The presence of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), methicillin-resistant <i>Staphylococcus</i> coagulase-negative (MRCoNS), vancomycin-resistant Enterococcus (VRE), extended-spectrum ß-lactamase-producing <i>Enterobacteriaceae</i> (BLEE), carbapenemase-producing <i>Enterobacteriaceae</i> (CPE), and colistin-resistant Enterobacteriaceae was investigated. The resistance genes of the isolated bacteria were characterised by specific PCRs depending on the microorganism to be studied, and in vitro antimicrobial resistance was determined using the broth microdilution method (Vitek<sup>®</sup>2 system bioMérieux<sup>®</sup>, Nurtingen, Germany). Results: MRSA prevalence was 73.21% (164 isolates). MRCoNS prevalence was 9.8% (22 isolates), <i>S. sciuri</i> being the prevalent species. Six isolates presented a 2.7% prevalence of extended-spectrum ß-lactamase-producing <i>Escherichia coli</i> (BLEE) in the CTX-M-1 group. No vancomycin-resistant <i>Enterococcus</i> (VRE), carbapenemase-producing <i>Enterobacteriaceae</i> (CRE), or colistin-resistant <i>Enterobacteriaceae</i> were isolated. Conclusion: we found a high presence of multiresistant bacteria, suggesting the need for increased control and surveillance of this type of strains in pig livestock and a better understanding of the possible transmission routes of these microorganisms through livestock products.https://www.mdpi.com/2306-7381/9/6/269pig livestockantimicrobial resistanceone-health |
| spellingShingle | Rossana Abreu Cristobalina Rodríguez-Álvarez Beatriz Castro-Hernandez Maria Lecuona-Fernández Juan Carlos González Yurena Rodríguez-Novo Maria de los Angeles Arias Rodríguez Prevalence and Characterisation of Multiresistant Bacterial Strains Isolated in Pigs from the Island of Tenerife Veterinary Sciences pig livestock antimicrobial resistance one-health |
| title | Prevalence and Characterisation of Multiresistant Bacterial Strains Isolated in Pigs from the Island of Tenerife |
| title_full | Prevalence and Characterisation of Multiresistant Bacterial Strains Isolated in Pigs from the Island of Tenerife |
| title_fullStr | Prevalence and Characterisation of Multiresistant Bacterial Strains Isolated in Pigs from the Island of Tenerife |
| title_full_unstemmed | Prevalence and Characterisation of Multiresistant Bacterial Strains Isolated in Pigs from the Island of Tenerife |
| title_short | Prevalence and Characterisation of Multiresistant Bacterial Strains Isolated in Pigs from the Island of Tenerife |
| title_sort | prevalence and characterisation of multiresistant bacterial strains isolated in pigs from the island of tenerife |
| topic | pig livestock antimicrobial resistance one-health |
| url | https://www.mdpi.com/2306-7381/9/6/269 |
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