Shockwaves Suppress Adipocyte Differentiation via Decrease in PPARγ
Adipogenesis is a crucial cellular process that contributes to the expansion of adipose tissue in obesity. Shockwaves are mechanical stimuli that transmit signals to cause biological responses. The purpose of this study is to evaluate the effects of shockwaves on adipogenesis. We treated 3T3L-1 cell...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-01-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/9/1/166 |
| _version_ | 1797709656075796480 |
|---|---|
| author | Wonkyoung Cho SeoYeon Kim Myeongsook Jeong Young Mi Park |
| author_facet | Wonkyoung Cho SeoYeon Kim Myeongsook Jeong Young Mi Park |
| author_sort | Wonkyoung Cho |
| collection | DOAJ |
| description | Adipogenesis is a crucial cellular process that contributes to the expansion of adipose tissue in obesity. Shockwaves are mechanical stimuli that transmit signals to cause biological responses. The purpose of this study is to evaluate the effects of shockwaves on adipogenesis. We treated 3T3L-1 cells and human primary preadipocytes for differentiation with or without shockwaves. Western blots and quantitative real-time reverse transcriptase PCR (qRT-PCR) for adipocyte markers including peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT-enhancer-binding proteins (C/EBPα) were performed. Extracellular adenosine triphosphate (ATP) and intracellular cyclic adenosine monophosphate (cAMP) levels, which are known to affect adipocyte differentiation, were measured. Shockwave treatment decreased intracellular lipid droplet accumulation in primary human preadipocytes and 3T3-L1 cells after 11−12 days of differentiation. Levels of key adipogenic transcriptional factors PPARγ and/or C/EBPα were lower in shockwave-treated human primary preadipocytes and 3T3L-1 cells after 12−13 days of differentiation than in shockwave-untreated cells. Shockwave treatment induced release of extracellular ATP from preadipocytes and decreased intracellular cAMP levels. Shockwave-treated preadipocytes showed a higher level of β-catenin and less PPARγ expression than shockwave-untreated cells. Supplementation with 8-bromo-cAMP analog after shockwave treatment rescued adipocyte differentiation by preventing the effect of shockwaves on β-catenin, Wnt10b mRNA, and PPARγ expression. Low-energy shockwaves suppressed adipocyte differentiation by decreasing PPARγ. Our study suggests an insight into potential uses of shockwave-treatment for obesity. |
| first_indexed | 2024-03-12T06:40:51Z |
| format | Article |
| id | doaj.art-2552ec9343a842768be0811a8f7d9b0d |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T06:40:51Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-2552ec9343a842768be0811a8f7d9b0d2023-09-03T01:00:45ZengMDPI AGCells2073-44092020-01-019116610.3390/cells9010166cells9010166Shockwaves Suppress Adipocyte Differentiation via Decrease in PPARγWonkyoung Cho0SeoYeon Kim1Myeongsook Jeong2Young Mi Park3Department of Molecular Medicine, College of Medicine, Ewha Womans University, Seoul 03760, KoreaDepartment of Molecular Medicine, College of Medicine, Ewha Womans University, Seoul 03760, KoreaDepartment of Molecular Medicine, College of Medicine, Ewha Womans University, Seoul 03760, KoreaDepartment of Molecular Medicine, College of Medicine, Ewha Womans University, Seoul 03760, KoreaAdipogenesis is a crucial cellular process that contributes to the expansion of adipose tissue in obesity. Shockwaves are mechanical stimuli that transmit signals to cause biological responses. The purpose of this study is to evaluate the effects of shockwaves on adipogenesis. We treated 3T3L-1 cells and human primary preadipocytes for differentiation with or without shockwaves. Western blots and quantitative real-time reverse transcriptase PCR (qRT-PCR) for adipocyte markers including peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT-enhancer-binding proteins (C/EBPα) were performed. Extracellular adenosine triphosphate (ATP) and intracellular cyclic adenosine monophosphate (cAMP) levels, which are known to affect adipocyte differentiation, were measured. Shockwave treatment decreased intracellular lipid droplet accumulation in primary human preadipocytes and 3T3-L1 cells after 11−12 days of differentiation. Levels of key adipogenic transcriptional factors PPARγ and/or C/EBPα were lower in shockwave-treated human primary preadipocytes and 3T3L-1 cells after 12−13 days of differentiation than in shockwave-untreated cells. Shockwave treatment induced release of extracellular ATP from preadipocytes and decreased intracellular cAMP levels. Shockwave-treated preadipocytes showed a higher level of β-catenin and less PPARγ expression than shockwave-untreated cells. Supplementation with 8-bromo-cAMP analog after shockwave treatment rescued adipocyte differentiation by preventing the effect of shockwaves on β-catenin, Wnt10b mRNA, and PPARγ expression. Low-energy shockwaves suppressed adipocyte differentiation by decreasing PPARγ. Our study suggests an insight into potential uses of shockwave-treatment for obesity.https://www.mdpi.com/2073-4409/9/1/166adipocyte differentiationshockwavespparγ3t3l-1camp |
| spellingShingle | Wonkyoung Cho SeoYeon Kim Myeongsook Jeong Young Mi Park Shockwaves Suppress Adipocyte Differentiation via Decrease in PPARγ Cells adipocyte differentiation shockwaves pparγ 3t3l-1 camp |
| title | Shockwaves Suppress Adipocyte Differentiation via Decrease in PPARγ |
| title_full | Shockwaves Suppress Adipocyte Differentiation via Decrease in PPARγ |
| title_fullStr | Shockwaves Suppress Adipocyte Differentiation via Decrease in PPARγ |
| title_full_unstemmed | Shockwaves Suppress Adipocyte Differentiation via Decrease in PPARγ |
| title_short | Shockwaves Suppress Adipocyte Differentiation via Decrease in PPARγ |
| title_sort | shockwaves suppress adipocyte differentiation via decrease in pparγ |
| topic | adipocyte differentiation shockwaves pparγ 3t3l-1 camp |
| url | https://www.mdpi.com/2073-4409/9/1/166 |
| work_keys_str_mv | AT wonkyoungcho shockwavessuppressadipocytedifferentiationviadecreaseinpparg AT seoyeonkim shockwavessuppressadipocytedifferentiationviadecreaseinpparg AT myeongsookjeong shockwavessuppressadipocytedifferentiationviadecreaseinpparg AT youngmipark shockwavessuppressadipocytedifferentiationviadecreaseinpparg |