The effect of a high- intensity running (12weeks) with bee pollen on gastrocnemius muscle ABCA1 and apoA1 mRNA expression in male rats
Abstract Background & Aims: The purpose of the current study was to investigate the effect of a high- intensity running (12weeks) with bee pollen on gastrocnemius muscle ABCA1 and apoA1 mRNA expression in male rats. Materials & Methods: Twenty_ four Wistar male rats (4-6 weeks old, 90-110...
| Format: | Article |
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| Language: | fas |
| Published: |
Tehran University of Medical Sciences
2016-10-01
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| Series: | Tehran University Medical Journal |
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| Online Access: | http://tumj.tums.ac.ir/browse.php?a_code=A-10-2512-1&slc_lang=en&sid=1 |
| Summary: | Abstract
Background & Aims: The purpose of the current study was to investigate the effect of a high- intensity running (12weeks) with bee pollen on gastrocnemius muscle ABCA1 and apoA1 mRNA expression in male rats.
Materials & Methods: Twenty_ four Wistar male rats (4-6 weeks old, 90-110 g weight) were used. Animals were randomly assigned into saline-control (SC, n=6), saline-training (ST, n=6), bee pollen- Control (BPC, n=6), and bee pollen-training (BPT, n=6) groups. Training groups have performed a high-intensity running program (30 m/min on 0% grade, 90min/day and 5 days/week) on a motor-driven treadmill for 12 weeks. Animals were orally fed with bee pollen extraction (500 mg/kg body weight) and saline solution for last 12 weeks. Rats were sacrificed 48 h after the last exercise session. A portion of gastrocnemius muscle were excised, cleaned and immediately frozen in liquid nitrogen and stored at -80 ° C until RNA extraction. Statistical analysis was performed using a two way ANOVA, and significance was accepted at P < 0.05.
Results: A significant differences have found in gastrocnemius muscle ABCA1 gene expression between SC and BPC groups (P<0.000), ST with BPC groups (P< 0.000), ST with BPT groups (P< 0.040) and BPC with BPT groups (P< 0.000).
Conclusion: The current results show that high-intensity running affected gastrocnemius muscle ABCA1 mRNA expression in different directions in saline (decrease) and BPT (increase) treated animals. Findings also indicate bee pollen consumption increase gastrocnemius muscle ABCA1 mRNA expression in rat. Also apoA1 was not expressed in the gastrocnemius muscle of rat.
Keywords: ABCA1, apoA1, Bee pollen, high- intensity running |
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| ISSN: | 1683-1764 1735-7322 |