Trials and Tribulations with VH Replacement
VH replacement is a type of antibody gene rearrangement in which an upstream heavy chain variable gene segment (VH) invades a pre-existing rearrangement (VDJ). In this Hypothesis and Theory article, we begin by reviewing the mechanism of VH replacement, its developmental timing and its potential bi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014-01-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fimmu.2014.00010/full |
| _version_ | 1819320132689723392 |
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| author | Wenzhao eMeng Sahana eJayaraman Zhang eBochao Gregory W. Schwartz Robert eDaber Uri eHershberg Uri eHershberg Alfred eGarfall Christopher S Carlson Christopher S Carlson Eline T. Luning Prak |
| author_facet | Wenzhao eMeng Sahana eJayaraman Zhang eBochao Gregory W. Schwartz Robert eDaber Uri eHershberg Uri eHershberg Alfred eGarfall Christopher S Carlson Christopher S Carlson Eline T. Luning Prak |
| author_sort | Wenzhao eMeng |
| collection | DOAJ |
| description | VH replacement is a type of antibody gene rearrangement in which an upstream heavy chain variable gene segment (VH) invades a pre-existing rearrangement (VDJ). In this Hypothesis and Theory article, we begin by reviewing the mechanism of VH replacement, its developmental timing and its potential biological consequences. Then we explore the hypothesis that specific sequence motifs called footprints reflect VH replacement vs. other processes. We provide a compilation of footprint sequences from different regions of the antibody heavy chain, include data from the literature and from a high throughput sequencing experiment to evaluate the significance of footprint sequences. We conclude by discussing the difficulties of attributing footprints to VH replacement. |
| first_indexed | 2024-12-24T11:14:43Z |
| format | Article |
| id | doaj.art-25589416ef3e40c8bd8c47c1728685b3 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-24T11:14:43Z |
| publishDate | 2014-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-25589416ef3e40c8bd8c47c1728685b32022-12-21T16:58:25ZengFrontiers Media S.A.Frontiers in Immunology1664-32242014-01-01510.3389/fimmu.2014.0001073470Trials and Tribulations with VH ReplacementWenzhao eMeng0Sahana eJayaraman1Zhang eBochao2Gregory W. Schwartz3Robert eDaber4Uri eHershberg5Uri eHershberg6Alfred eGarfall7Christopher S Carlson8Christopher S Carlson9Eline T. Luning Prak10Perelman School of Medicine, University of PennsylvaniaPerelman School of Medicine, University of PennsylvaniaDrexel University School of Biomedical Engineering, Science and Health SystemsDrexel University School of Biomedical Engineering, Science and Health SystemsPerelman School of Medicine, University of PennsylvaniaDrexel University School of Biomedical Engineering, Science and Health SystemsCollege of Medicine, Drexel UniversityPerelman School of Medicine, University of PennsylvaniaThe Fred Hutchinson Cancer Research CenterAdaptive BiotechnologiesPerelman School of Medicine, University of PennsylvaniaVH replacement is a type of antibody gene rearrangement in which an upstream heavy chain variable gene segment (VH) invades a pre-existing rearrangement (VDJ). In this Hypothesis and Theory article, we begin by reviewing the mechanism of VH replacement, its developmental timing and its potential biological consequences. Then we explore the hypothesis that specific sequence motifs called footprints reflect VH replacement vs. other processes. We provide a compilation of footprint sequences from different regions of the antibody heavy chain, include data from the literature and from a high throughput sequencing experiment to evaluate the significance of footprint sequences. We conclude by discussing the difficulties of attributing footprints to VH replacement.http://journal.frontiersin.org/Journal/10.3389/fimmu.2014.00010/fullV(D)J Recombinationreceptor editinghigh throughput sequencingVH replacementCDR3H |
| spellingShingle | Wenzhao eMeng Sahana eJayaraman Zhang eBochao Gregory W. Schwartz Robert eDaber Uri eHershberg Uri eHershberg Alfred eGarfall Christopher S Carlson Christopher S Carlson Eline T. Luning Prak Trials and Tribulations with VH Replacement Frontiers in Immunology V(D)J Recombination receptor editing high throughput sequencing VH replacement CDR3H |
| title | Trials and Tribulations with VH Replacement |
| title_full | Trials and Tribulations with VH Replacement |
| title_fullStr | Trials and Tribulations with VH Replacement |
| title_full_unstemmed | Trials and Tribulations with VH Replacement |
| title_short | Trials and Tribulations with VH Replacement |
| title_sort | trials and tribulations with vh replacement |
| topic | V(D)J Recombination receptor editing high throughput sequencing VH replacement CDR3H |
| url | http://journal.frontiersin.org/Journal/10.3389/fimmu.2014.00010/full |
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