Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially Organized
Bimodal karyotypes are characterized by the presence of two sets of chromosomes of contrasting size. Eleutherine bulbosa (2n = 12) presents a bimodal karyotype with a large chromosome pair, which has a pericentric inversion in permanent heterozygosity with suppressed recombination, and five pairs of...
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.01170/full |
| _version_ | 1811204179340820480 |
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| author | Mariana Báez Magdalena Vaio Steven Dreissig Veit Schubert Andreas Houben Andrea Pedrosa-Harand |
| author_facet | Mariana Báez Magdalena Vaio Steven Dreissig Veit Schubert Andreas Houben Andrea Pedrosa-Harand |
| author_sort | Mariana Báez |
| collection | DOAJ |
| description | Bimodal karyotypes are characterized by the presence of two sets of chromosomes of contrasting size. Eleutherine bulbosa (2n = 12) presents a bimodal karyotype with a large chromosome pair, which has a pericentric inversion in permanent heterozygosity with suppressed recombination, and five pairs of three to four times smaller chromosomes. Aiming to understand whether high copy number sequence composition differs between both chromosome sets, we investigated the repetitive DNA fraction of E. bulbosa and compared it to the chromosomal organization of the related Eleutherine latifolia species, not containing the pericentric inversion. We also compared the repetitive sequence proportions between the heteromorphic large chromosomes of E. bulbosa and between E. bulbosa and E. latifolia to understand the influence of the chromosome inversion on the dynamics of repetitive sequences. The most abundant repetitive families of the genome showed a similar chromosomal distribution in both homologs of the large pair and in both species, apparently not influenced by the species-specific inversions. The repeat families Ebusat1 and Ebusat4 are localized interstitially only on the large chromosome pair, while Ebusat2 is located in the centromeric region of all chromosomes. The four most abundant retrotransposon lineages are accumulated in the large chromosome pair. Replication timing and distribution of epigenetic and transcriptional marks differ between large and small chromosomes. The differential distribution of retroelements appears to be related to the bimodal condition and is not influenced by the nonrecombining chromosome inversions in these species. Thus, the large and small chromosome subgenomes of the bimodal Eleutherine karyotype are differentially organized and probably evolved by repetitive sequences accumulation on the large chromosome set. |
| first_indexed | 2024-04-12T03:08:37Z |
| format | Article |
| id | doaj.art-255990e8936345cbb797cbcc0dbd9fc6 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T03:08:37Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-255990e8936345cbb797cbcc0dbd9fc62022-12-22T03:50:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-10-011010.3389/fpls.2019.01170477201Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially OrganizedMariana Báez0Magdalena Vaio1Steven Dreissig2Veit Schubert3Andreas Houben4Andrea Pedrosa-Harand5Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Federal University of Pernambuco, Recife, BrazilLaboratory of Genetics, Department of Plant Biology, College of Agronomy, University of the Republic, Montevideo, UruguayDepartment of Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanyDepartment of Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanyDepartment of Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanyLaboratory of Plant Cytogenetics and Evolution, Department of Botany, Federal University of Pernambuco, Recife, BrazilBimodal karyotypes are characterized by the presence of two sets of chromosomes of contrasting size. Eleutherine bulbosa (2n = 12) presents a bimodal karyotype with a large chromosome pair, which has a pericentric inversion in permanent heterozygosity with suppressed recombination, and five pairs of three to four times smaller chromosomes. Aiming to understand whether high copy number sequence composition differs between both chromosome sets, we investigated the repetitive DNA fraction of E. bulbosa and compared it to the chromosomal organization of the related Eleutherine latifolia species, not containing the pericentric inversion. We also compared the repetitive sequence proportions between the heteromorphic large chromosomes of E. bulbosa and between E. bulbosa and E. latifolia to understand the influence of the chromosome inversion on the dynamics of repetitive sequences. The most abundant repetitive families of the genome showed a similar chromosomal distribution in both homologs of the large pair and in both species, apparently not influenced by the species-specific inversions. The repeat families Ebusat1 and Ebusat4 are localized interstitially only on the large chromosome pair, while Ebusat2 is located in the centromeric region of all chromosomes. The four most abundant retrotransposon lineages are accumulated in the large chromosome pair. Replication timing and distribution of epigenetic and transcriptional marks differ between large and small chromosomes. The differential distribution of retroelements appears to be related to the bimodal condition and is not influenced by the nonrecombining chromosome inversions in these species. Thus, the large and small chromosome subgenomes of the bimodal Eleutherine karyotype are differentially organized and probably evolved by repetitive sequences accumulation on the large chromosome set.https://www.frontiersin.org/article/10.3389/fpls.2019.01170/fullretrotransposonssatellite DNArepetitive sequences accumulationDNA replicationhistone modificationinversion |
| spellingShingle | Mariana Báez Magdalena Vaio Steven Dreissig Veit Schubert Andreas Houben Andrea Pedrosa-Harand Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially Organized Frontiers in Plant Science retrotransposons satellite DNA repetitive sequences accumulation DNA replication histone modification inversion |
| title | Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially Organized |
| title_full | Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially Organized |
| title_fullStr | Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially Organized |
| title_full_unstemmed | Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially Organized |
| title_short | Together But Different: The Subgenomes of the Bimodal Eleutherine Karyotypes Are Differentially Organized |
| title_sort | together but different the subgenomes of the bimodal eleutherine karyotypes are differentially organized |
| topic | retrotransposons satellite DNA repetitive sequences accumulation DNA replication histone modification inversion |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.01170/full |
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