Customizing Sanitization Protocols for Food-Borne Pathogens Based on Biofilm Formation, Surfaces and Disinfectants—Their Two- and Three-Way Interactions
Food-borne pathogens are a serious challenge in food handling, processing, and packaging systems. The growth of microbial biofilms on food handling surfaces further complicates the management of the microbial contamination of food. Microorganisms within biofilms are difficult to eradicate with chemi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Applied Microbiology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-8007/4/1/3 |
| _version_ | 1797242259513540608 |
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| author | Manju Kundu Amin Omar Brenton Buziak Nick Allan Lyriam Marques Merle Olson Ronald Howard Michael W. Harding |
| author_facet | Manju Kundu Amin Omar Brenton Buziak Nick Allan Lyriam Marques Merle Olson Ronald Howard Michael W. Harding |
| author_sort | Manju Kundu |
| collection | DOAJ |
| description | Food-borne pathogens are a serious challenge in food handling, processing, and packaging systems. The growth of microbial biofilms on food handling surfaces further complicates the management of the microbial contamination of food. Microorganisms within biofilms are difficult to eradicate with chemical disinfectants, with an increased likelihood of survival and the subsequent contamination of food. Therefore, a biofilm approach is needed in food safety and hygiene studies. Since many factors, such as strain, cell density, surface type and texture, environmental stress, and so forth, can affect biofilm formation and disinfectant efficacy, we evaluated the responses of biofilms formed by three food-borne bacterial pathogens on eight hard surfaces to seven chemical disinfectants. The three bacteria showed different capacities to colonize the surfaces. Similarly, chemical disinfectants also varied in efficacy, on surfaces and with pathogen species. One-, two-, and three-way interactions of strain, surface, and disinfectant were observed. The results generated demonstrate that the fine-tuning of sanitization strategies along the food production, processing, and packaging chain can be achieved in specific scenarios by accounting for two- and three-way interactions among bacteria, surface, and disinfectant. |
| first_indexed | 2024-04-24T18:36:23Z |
| format | Article |
| id | doaj.art-255a4ceb21624fdfbf7dacbcd782cd99 |
| institution | Directory Open Access Journal |
| issn | 2673-8007 |
| language | English |
| last_indexed | 2024-04-24T18:36:23Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Microbiology |
| spelling | doaj.art-255a4ceb21624fdfbf7dacbcd782cd992024-03-27T13:18:55ZengMDPI AGApplied Microbiology2673-80072023-12-0141274610.3390/applmicrobiol4010003Customizing Sanitization Protocols for Food-Borne Pathogens Based on Biofilm Formation, Surfaces and Disinfectants—Their Two- and Three-Way InteractionsManju Kundu0Amin Omar1Brenton Buziak2Nick Allan3Lyriam Marques4Merle Olson5Ronald Howard6Michael W. Harding7Crop Diversification Centre South, Alberta Agriculture and Irrigation, 301 Horticultural Station Road East, Brooks, AB T1R 1E6, CanadaDepartment of Pharmaceutical Sciences and Pharmaceutics, Faculty of Pharmacy, P.O. Box 541350, Applied Science Private University, Amman 11937, JordanInnovotech Inc., Edmonton, AB T6N 1H1, CanadaChinook Contract Research, Airdrie, AB T4A 0C3, CanadaHydroqual Laboratories Ltd., Calgary, AB T2H 2K1, CanadaChinook Contract Research, Airdrie, AB T4A 0C3, CanadaRJH Ag Research Solutions, Brooks, AB T1R 1C3, CanadaCrop Diversification Centre South, Alberta Agriculture and Irrigation, 301 Horticultural Station Road East, Brooks, AB T1R 1E6, CanadaFood-borne pathogens are a serious challenge in food handling, processing, and packaging systems. The growth of microbial biofilms on food handling surfaces further complicates the management of the microbial contamination of food. Microorganisms within biofilms are difficult to eradicate with chemical disinfectants, with an increased likelihood of survival and the subsequent contamination of food. Therefore, a biofilm approach is needed in food safety and hygiene studies. Since many factors, such as strain, cell density, surface type and texture, environmental stress, and so forth, can affect biofilm formation and disinfectant efficacy, we evaluated the responses of biofilms formed by three food-borne bacterial pathogens on eight hard surfaces to seven chemical disinfectants. The three bacteria showed different capacities to colonize the surfaces. Similarly, chemical disinfectants also varied in efficacy, on surfaces and with pathogen species. One-, two-, and three-way interactions of strain, surface, and disinfectant were observed. The results generated demonstrate that the fine-tuning of sanitization strategies along the food production, processing, and packaging chain can be achieved in specific scenarios by accounting for two- and three-way interactions among bacteria, surface, and disinfectant.https://www.mdpi.com/2673-8007/4/1/3disinfectionsurface-associated<i>Escherichia coli</i> serotype O157:H7Listeria monocytogenes<i>Salmonella choleraesuis</i> |
| spellingShingle | Manju Kundu Amin Omar Brenton Buziak Nick Allan Lyriam Marques Merle Olson Ronald Howard Michael W. Harding Customizing Sanitization Protocols for Food-Borne Pathogens Based on Biofilm Formation, Surfaces and Disinfectants—Their Two- and Three-Way Interactions Applied Microbiology disinfection surface-associated <i>Escherichia coli</i> serotype O157:H7 Listeria monocytogenes <i>Salmonella choleraesuis</i> |
| title | Customizing Sanitization Protocols for Food-Borne Pathogens Based on Biofilm Formation, Surfaces and Disinfectants—Their Two- and Three-Way Interactions |
| title_full | Customizing Sanitization Protocols for Food-Borne Pathogens Based on Biofilm Formation, Surfaces and Disinfectants—Their Two- and Three-Way Interactions |
| title_fullStr | Customizing Sanitization Protocols for Food-Borne Pathogens Based on Biofilm Formation, Surfaces and Disinfectants—Their Two- and Three-Way Interactions |
| title_full_unstemmed | Customizing Sanitization Protocols for Food-Borne Pathogens Based on Biofilm Formation, Surfaces and Disinfectants—Their Two- and Three-Way Interactions |
| title_short | Customizing Sanitization Protocols for Food-Borne Pathogens Based on Biofilm Formation, Surfaces and Disinfectants—Their Two- and Three-Way Interactions |
| title_sort | customizing sanitization protocols for food borne pathogens based on biofilm formation surfaces and disinfectants their two and three way interactions |
| topic | disinfection surface-associated <i>Escherichia coli</i> serotype O157:H7 Listeria monocytogenes <i>Salmonella choleraesuis</i> |
| url | https://www.mdpi.com/2673-8007/4/1/3 |
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