TgMORN2, a MORN Family Protein Involved in the Regulation of Endoplasmic Reticulum Stress in <i>Toxoplasma gondii</i>
MORN proteins play a key role in the cytoskeletal structure of eukaryotes and are essential for the close arrangement of the endoplasmic reticulum and plasma membrane. A gene with nine MORN motifs (TGGT1_292120, named Tg<i>MORN2</i>) was identified in the <i>Toxoplasma gondii</i...
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/12/10228 |
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| author | Jinxuan Li Qianqian Xiao Qianqian Tan Junpeng Chen Lingyu Sun Xiao Chen Ziyu Chu Hongxia Wu Zhenzhao Zhang Hongmei Li Xiaomin Zhao Xiao Zhang |
| author_facet | Jinxuan Li Qianqian Xiao Qianqian Tan Junpeng Chen Lingyu Sun Xiao Chen Ziyu Chu Hongxia Wu Zhenzhao Zhang Hongmei Li Xiaomin Zhao Xiao Zhang |
| author_sort | Jinxuan Li |
| collection | DOAJ |
| description | MORN proteins play a key role in the cytoskeletal structure of eukaryotes and are essential for the close arrangement of the endoplasmic reticulum and plasma membrane. A gene with nine MORN motifs (TGGT1_292120, named Tg<i>MORN2</i>) was identified in the <i>Toxoplasma gondii</i> genome; it was presumed to belong to the MORN protein family and to have the function of forming the cytoskeleton, which affects the survival of <i>T. gondii</i>. However, the genetic deletion of <i>MORN2</i> did not noticeably affect parasite growth and virulence. Using adjacent protein labeling techniques, we identified a network of TgMORN2 interactions, which mainly included endoplasmic reticulum stress (ER stress)-related proteins. In exploring these data, we found that the pathogenicity of the KO-Tg<i>MORN2</i> strain was significantly reduced in the case of tunicamycin-induced ER stress. Reticulon TgRTN (TGGT1_226430) and tubulin β-Tubulin were identified as interaction proteins of TgMORN2. Collectively, TgMORN2 plays a role in ER stress, which lays a foundation for further research on the function of the MORN protein in <i>T. gondii</i>. |
| first_indexed | 2024-03-11T02:21:06Z |
| format | Article |
| id | doaj.art-255c50da44254e71b895d118be4cbd54 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T02:21:06Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-255c50da44254e71b895d118be4cbd542023-11-18T10:50:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124121022810.3390/ijms241210228TgMORN2, a MORN Family Protein Involved in the Regulation of Endoplasmic Reticulum Stress in <i>Toxoplasma gondii</i>Jinxuan Li0Qianqian Xiao1Qianqian Tan2Junpeng Chen3Lingyu Sun4Xiao Chen5Ziyu Chu6Hongxia Wu7Zhenzhao Zhang8Hongmei Li9Xiaomin Zhao10Xiao Zhang11Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaMORN proteins play a key role in the cytoskeletal structure of eukaryotes and are essential for the close arrangement of the endoplasmic reticulum and plasma membrane. A gene with nine MORN motifs (TGGT1_292120, named Tg<i>MORN2</i>) was identified in the <i>Toxoplasma gondii</i> genome; it was presumed to belong to the MORN protein family and to have the function of forming the cytoskeleton, which affects the survival of <i>T. gondii</i>. However, the genetic deletion of <i>MORN2</i> did not noticeably affect parasite growth and virulence. Using adjacent protein labeling techniques, we identified a network of TgMORN2 interactions, which mainly included endoplasmic reticulum stress (ER stress)-related proteins. In exploring these data, we found that the pathogenicity of the KO-Tg<i>MORN2</i> strain was significantly reduced in the case of tunicamycin-induced ER stress. Reticulon TgRTN (TGGT1_226430) and tubulin β-Tubulin were identified as interaction proteins of TgMORN2. Collectively, TgMORN2 plays a role in ER stress, which lays a foundation for further research on the function of the MORN protein in <i>T. gondii</i>.https://www.mdpi.com/1422-0067/24/12/10228<i>Toxoplasma gondii</i>TgMORN2endoplasmic reticulum stress |
| spellingShingle | Jinxuan Li Qianqian Xiao Qianqian Tan Junpeng Chen Lingyu Sun Xiao Chen Ziyu Chu Hongxia Wu Zhenzhao Zhang Hongmei Li Xiaomin Zhao Xiao Zhang TgMORN2, a MORN Family Protein Involved in the Regulation of Endoplasmic Reticulum Stress in <i>Toxoplasma gondii</i> International Journal of Molecular Sciences <i>Toxoplasma gondii</i> TgMORN2 endoplasmic reticulum stress |
| title | TgMORN2, a MORN Family Protein Involved in the Regulation of Endoplasmic Reticulum Stress in <i>Toxoplasma gondii</i> |
| title_full | TgMORN2, a MORN Family Protein Involved in the Regulation of Endoplasmic Reticulum Stress in <i>Toxoplasma gondii</i> |
| title_fullStr | TgMORN2, a MORN Family Protein Involved in the Regulation of Endoplasmic Reticulum Stress in <i>Toxoplasma gondii</i> |
| title_full_unstemmed | TgMORN2, a MORN Family Protein Involved in the Regulation of Endoplasmic Reticulum Stress in <i>Toxoplasma gondii</i> |
| title_short | TgMORN2, a MORN Family Protein Involved in the Regulation of Endoplasmic Reticulum Stress in <i>Toxoplasma gondii</i> |
| title_sort | tgmorn2 a morn family protein involved in the regulation of endoplasmic reticulum stress in i toxoplasma gondii i |
| topic | <i>Toxoplasma gondii</i> TgMORN2 endoplasmic reticulum stress |
| url | https://www.mdpi.com/1422-0067/24/12/10228 |
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