Ikaros and RAG-2-mediated antisense transcription are responsible for lymphocyte-specific inactivation of NWC promoter.
Recombination activating gene-2 (RAG-2) and NWC are strongly evolutionarily conserved overlapping genes which are convergently transcribed. In non-lymphoid cells the NWC promoter is active whereas in lymphocytes it is inactive due to the DNA methylation. Analysing the mechanism responsible for lymph...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4157847?pdf=render |
| _version_ | 1818249568679100416 |
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| author | Agnieszka Laszkiewicz Łukasz Bzdzion Monika Kasztura Lukasz Snieżewski Sylwia Janik Paweł Kisielow Małgorzata Cebrat |
| author_facet | Agnieszka Laszkiewicz Łukasz Bzdzion Monika Kasztura Lukasz Snieżewski Sylwia Janik Paweł Kisielow Małgorzata Cebrat |
| author_sort | Agnieszka Laszkiewicz |
| collection | DOAJ |
| description | Recombination activating gene-2 (RAG-2) and NWC are strongly evolutionarily conserved overlapping genes which are convergently transcribed. In non-lymphoid cells the NWC promoter is active whereas in lymphocytes it is inactive due to the DNA methylation. Analysing the mechanism responsible for lymphocyte-specific methylation and inactivation of NWC promoter we found that Ikaros, a lymphocyte-specific transcription factor, acts as a repressor of NWC promoter--thus identifying a new Ikaros target--but is insufficient for inducing its methylation which depends on the antisense transcription driven by RAG-2 promoter. Possible implications of these observations for understanding evolutionary mechanisms leading to lymphocyte specific expression of RAG genes are discussed. |
| first_indexed | 2024-12-12T15:38:34Z |
| format | Article |
| id | doaj.art-255cccf305694eef882d4f0ac2b30bee |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T15:38:34Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-255cccf305694eef882d4f0ac2b30bee2022-12-22T00:19:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10692710.1371/journal.pone.0106927Ikaros and RAG-2-mediated antisense transcription are responsible for lymphocyte-specific inactivation of NWC promoter.Agnieszka LaszkiewiczŁukasz BzdzionMonika KaszturaLukasz SnieżewskiSylwia JanikPaweł KisielowMałgorzata CebratRecombination activating gene-2 (RAG-2) and NWC are strongly evolutionarily conserved overlapping genes which are convergently transcribed. In non-lymphoid cells the NWC promoter is active whereas in lymphocytes it is inactive due to the DNA methylation. Analysing the mechanism responsible for lymphocyte-specific methylation and inactivation of NWC promoter we found that Ikaros, a lymphocyte-specific transcription factor, acts as a repressor of NWC promoter--thus identifying a new Ikaros target--but is insufficient for inducing its methylation which depends on the antisense transcription driven by RAG-2 promoter. Possible implications of these observations for understanding evolutionary mechanisms leading to lymphocyte specific expression of RAG genes are discussed.http://europepmc.org/articles/PMC4157847?pdf=render |
| spellingShingle | Agnieszka Laszkiewicz Łukasz Bzdzion Monika Kasztura Lukasz Snieżewski Sylwia Janik Paweł Kisielow Małgorzata Cebrat Ikaros and RAG-2-mediated antisense transcription are responsible for lymphocyte-specific inactivation of NWC promoter. PLoS ONE |
| title | Ikaros and RAG-2-mediated antisense transcription are responsible for lymphocyte-specific inactivation of NWC promoter. |
| title_full | Ikaros and RAG-2-mediated antisense transcription are responsible for lymphocyte-specific inactivation of NWC promoter. |
| title_fullStr | Ikaros and RAG-2-mediated antisense transcription are responsible for lymphocyte-specific inactivation of NWC promoter. |
| title_full_unstemmed | Ikaros and RAG-2-mediated antisense transcription are responsible for lymphocyte-specific inactivation of NWC promoter. |
| title_short | Ikaros and RAG-2-mediated antisense transcription are responsible for lymphocyte-specific inactivation of NWC promoter. |
| title_sort | ikaros and rag 2 mediated antisense transcription are responsible for lymphocyte specific inactivation of nwc promoter |
| url | http://europepmc.org/articles/PMC4157847?pdf=render |
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