Effect of Vipera berus berus and Vipera berus nikolskii venom on proteolytic balance in the tissue of the adrenal glands and testicles of rats
Background: Vipera berus berus and Vipera berus nikolskii are the most widely spread Old World vipers in Europe, who contribute to almost all cases of snakebites on this territory. Their venoms are known to cause proteolysis, thrombocytopenia, induction of neurotoxicity and haemorrhage, among which...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Biomedical and Biotechnology Research Journal |
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| Online Access: | http://www.bmbtrj.org/article.asp?issn=2588-9834;year=2022;volume=6;issue=4;spage=543;epage=549;aulast=Palamarchuk |
| _version_ | 1797955047018987520 |
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| author | Mariia Palamarchuk Timur Niyazmetov Tetiana Halenova Natalia Raksha Oleksandr Maievskyi Iryna Dzevulska Kateryna Zaichko Olexii Savchuk Ludmila Ostapchenko |
| author_facet | Mariia Palamarchuk Timur Niyazmetov Tetiana Halenova Natalia Raksha Oleksandr Maievskyi Iryna Dzevulska Kateryna Zaichko Olexii Savchuk Ludmila Ostapchenko |
| author_sort | Mariia Palamarchuk |
| collection | DOAJ |
| description | Background: Vipera berus berus and Vipera berus nikolskii are the most widely spread Old World vipers in Europe, who contribute to almost all cases of snakebites on this territory. Their venoms are known to cause proteolysis, thrombocytopenia, induction of neurotoxicity and haemorrhage, among which proteolytic action constitute to the possible affection of all metabolic pathways in the targeted organs and in the whole organism. Methods: The influence of the viper venoms on the adrenal glands and testicles of injected rats was studied through the analysis of the concentration of proteins (Bradford method), evaluation of the qualitative protein profile (disc electrophoresis), distribution of active proteolytic molecules in the studied tissues (enzyme electrophoresis) and estimation of the appearance of low molecular weight molecules. Results: The decline of the number of the total protein, the alterations of the protein composition and the enzymatic redistribution were declared to appear in these organs under the action of the studied venoms proving the assumption of the proteolysis activation. Whereas, the increase in the level of low molecular weight molecules has emerged showing the onset of the state of toxicity. Conclusion: the study of the influence of the V. berus berus and V. berus nikolskii venoms on the protein balance of adrenal glands and testicles was performed, showing the impact on the amount of total proteins, its redistribution and connection with toxicity and proving the onset of pathological process. |
| first_indexed | 2024-04-10T23:28:12Z |
| format | Article |
| id | doaj.art-2563330faade420eb55e2d7eee8ac141 |
| institution | Directory Open Access Journal |
| issn | 2588-9834 2588-9842 |
| language | English |
| last_indexed | 2024-04-10T23:28:12Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Biomedical and Biotechnology Research Journal |
| spelling | doaj.art-2563330faade420eb55e2d7eee8ac1412023-01-12T11:21:07ZengWolters Kluwer Medknow PublicationsBiomedical and Biotechnology Research Journal2588-98342588-98422022-01-016454354910.4103/bbrj.bbrj_287_22Effect of Vipera berus berus and Vipera berus nikolskii venom on proteolytic balance in the tissue of the adrenal glands and testicles of ratsMariia PalamarchukTimur NiyazmetovTetiana HalenovaNatalia RakshaOleksandr MaievskyiIryna DzevulskaKateryna ZaichkoOlexii SavchukLudmila OstapchenkoBackground: Vipera berus berus and Vipera berus nikolskii are the most widely spread Old World vipers in Europe, who contribute to almost all cases of snakebites on this territory. Their venoms are known to cause proteolysis, thrombocytopenia, induction of neurotoxicity and haemorrhage, among which proteolytic action constitute to the possible affection of all metabolic pathways in the targeted organs and in the whole organism. Methods: The influence of the viper venoms on the adrenal glands and testicles of injected rats was studied through the analysis of the concentration of proteins (Bradford method), evaluation of the qualitative protein profile (disc electrophoresis), distribution of active proteolytic molecules in the studied tissues (enzyme electrophoresis) and estimation of the appearance of low molecular weight molecules. Results: The decline of the number of the total protein, the alterations of the protein composition and the enzymatic redistribution were declared to appear in these organs under the action of the studied venoms proving the assumption of the proteolysis activation. Whereas, the increase in the level of low molecular weight molecules has emerged showing the onset of the state of toxicity. Conclusion: the study of the influence of the V. berus berus and V. berus nikolskii venoms on the protein balance of adrenal glands and testicles was performed, showing the impact on the amount of total proteins, its redistribution and connection with toxicity and proving the onset of pathological process.http://www.bmbtrj.org/article.asp?issn=2588-9834;year=2022;volume=6;issue=4;spage=543;epage=549;aulast=Palamarchukproteolysistoxicityvenomviperviperinae |
| spellingShingle | Mariia Palamarchuk Timur Niyazmetov Tetiana Halenova Natalia Raksha Oleksandr Maievskyi Iryna Dzevulska Kateryna Zaichko Olexii Savchuk Ludmila Ostapchenko Effect of Vipera berus berus and Vipera berus nikolskii venom on proteolytic balance in the tissue of the adrenal glands and testicles of rats Biomedical and Biotechnology Research Journal proteolysis toxicity venom viper viperinae |
| title | Effect of Vipera berus berus and Vipera berus nikolskii venom on proteolytic balance in the tissue of the adrenal glands and testicles of rats |
| title_full | Effect of Vipera berus berus and Vipera berus nikolskii venom on proteolytic balance in the tissue of the adrenal glands and testicles of rats |
| title_fullStr | Effect of Vipera berus berus and Vipera berus nikolskii venom on proteolytic balance in the tissue of the adrenal glands and testicles of rats |
| title_full_unstemmed | Effect of Vipera berus berus and Vipera berus nikolskii venom on proteolytic balance in the tissue of the adrenal glands and testicles of rats |
| title_short | Effect of Vipera berus berus and Vipera berus nikolskii venom on proteolytic balance in the tissue of the adrenal glands and testicles of rats |
| title_sort | effect of vipera berus berus and vipera berus nikolskii venom on proteolytic balance in the tissue of the adrenal glands and testicles of rats |
| topic | proteolysis toxicity venom viper viperinae |
| url | http://www.bmbtrj.org/article.asp?issn=2588-9834;year=2022;volume=6;issue=4;spage=543;epage=549;aulast=Palamarchuk |
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