Antisense noncoding mitochondrial RNA-2 gives rise to miR-4485-3p by Dicer processing in vitro
Abstract Background The antisense noncoding mitochondrial RNAs (ASncmtRNAs) derive from the mitochondrial 16S gene. Knockdown of these transcripts with chemically-modified antisense oligonucleotides induces proliferative arrest, apoptosis and invasiveness reduction in tumor but not normal cells. One...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | Biological Research |
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| Online Access: | https://doi.org/10.1186/s40659-021-00356-0 |
| _version_ | 1818723370164813824 |
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| author | Nicole Farfán Nicole Sanhueza Macarena Briones Luis O. Burzio Verónica A. Burzio |
| author_facet | Nicole Farfán Nicole Sanhueza Macarena Briones Luis O. Burzio Verónica A. Burzio |
| author_sort | Nicole Farfán |
| collection | DOAJ |
| description | Abstract Background The antisense noncoding mitochondrial RNAs (ASncmtRNAs) derive from the mitochondrial 16S gene. Knockdown of these transcripts with chemically-modified antisense oligonucleotides induces proliferative arrest, apoptosis and invasiveness reduction in tumor but not normal cells. One of these transcripts, ASncmtRNA-2, contains the complete and identical sequence of hsa-miR-4485-3p and, upon knockdown of this transcript, there is a strong increase in levels of this miRNA, suggesting ASncmtRNA-2 as a source for miR-4485-3p, which is supported by several evidences from our group and others, in the ex vivo setting. Results Here we show that incubation of in vitro-transcribed ASncmtRNA-2 with recombinant Dicer produces RNA fragments corresponding to hsa-miR-4485-3p, showing that Dicer binds to and processes ASncmtRNA-2, strongly supporting the hypothesis that ASncmtRNA-2 acts as a precursor for miR-4485-3p. Conclusion The in vitro results presented here strengthen the hypothesis that miR-4485-3p is derived from ASncmtRNA-2 by Dicer processing. Since miR-4485-3p is classified as a tumor suppressor miRNA, this evidence strengthens the application of ASncmtRNA knockdown for cancer therapy. |
| first_indexed | 2024-12-17T21:09:26Z |
| format | Article |
| id | doaj.art-2563914fbc02406e9c6e32e7565a9045 |
| institution | Directory Open Access Journal |
| issn | 0717-6287 |
| language | English |
| last_indexed | 2024-12-17T21:09:26Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-2563914fbc02406e9c6e32e7565a90452022-12-21T21:32:30ZengBMCBiological Research0717-62872021-10-015411710.1186/s40659-021-00356-0Antisense noncoding mitochondrial RNA-2 gives rise to miR-4485-3p by Dicer processing in vitroNicole Farfán0Nicole Sanhueza1Macarena Briones2Luis O. Burzio3Verónica A. Burzio4Fundación Ciencia & Vida/Andes Biotechnologies SpAFundación Ciencia & Vida/Andes Biotechnologies SpAFundación Ciencia & Vida/Andes Biotechnologies SpAFundación Ciencia & Vida/Andes Biotechnologies SpAFundación Ciencia & Vida/Andes Biotechnologies SpAAbstract Background The antisense noncoding mitochondrial RNAs (ASncmtRNAs) derive from the mitochondrial 16S gene. Knockdown of these transcripts with chemically-modified antisense oligonucleotides induces proliferative arrest, apoptosis and invasiveness reduction in tumor but not normal cells. One of these transcripts, ASncmtRNA-2, contains the complete and identical sequence of hsa-miR-4485-3p and, upon knockdown of this transcript, there is a strong increase in levels of this miRNA, suggesting ASncmtRNA-2 as a source for miR-4485-3p, which is supported by several evidences from our group and others, in the ex vivo setting. Results Here we show that incubation of in vitro-transcribed ASncmtRNA-2 with recombinant Dicer produces RNA fragments corresponding to hsa-miR-4485-3p, showing that Dicer binds to and processes ASncmtRNA-2, strongly supporting the hypothesis that ASncmtRNA-2 acts as a precursor for miR-4485-3p. Conclusion The in vitro results presented here strengthen the hypothesis that miR-4485-3p is derived from ASncmtRNA-2 by Dicer processing. Since miR-4485-3p is classified as a tumor suppressor miRNA, this evidence strengthens the application of ASncmtRNA knockdown for cancer therapy.https://doi.org/10.1186/s40659-021-00356-0ncRNAmiRNACancerlncRNADicerCloning |
| spellingShingle | Nicole Farfán Nicole Sanhueza Macarena Briones Luis O. Burzio Verónica A. Burzio Antisense noncoding mitochondrial RNA-2 gives rise to miR-4485-3p by Dicer processing in vitro Biological Research ncRNA miRNA Cancer lncRNA Dicer Cloning |
| title | Antisense noncoding mitochondrial RNA-2 gives rise to miR-4485-3p by Dicer processing in vitro |
| title_full | Antisense noncoding mitochondrial RNA-2 gives rise to miR-4485-3p by Dicer processing in vitro |
| title_fullStr | Antisense noncoding mitochondrial RNA-2 gives rise to miR-4485-3p by Dicer processing in vitro |
| title_full_unstemmed | Antisense noncoding mitochondrial RNA-2 gives rise to miR-4485-3p by Dicer processing in vitro |
| title_short | Antisense noncoding mitochondrial RNA-2 gives rise to miR-4485-3p by Dicer processing in vitro |
| title_sort | antisense noncoding mitochondrial rna 2 gives rise to mir 4485 3p by dicer processing in vitro |
| topic | ncRNA miRNA Cancer lncRNA Dicer Cloning |
| url | https://doi.org/10.1186/s40659-021-00356-0 |
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