Transcriptome Analysis of Maternal Gene Transcripts in Unfertilized Eggs of <i>Misgurnus anguillicaudatus</i> and Identification of Immune-Related Maternal Genes
Maternal genes are important in directing early development and determining egg quality in fish. We here report the de novo transcriptome from four tissue libraries of the cyprinid loach, <i>Misgurnus anguillicaudatus</i>, and for the first time identified maternal gene transcripts in un...
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MDPI AG
2020-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/11/3872 |
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| author | Chan-Hee Kim Eun Jeong Kim Chaehwa Seo Yoon Kwon Nam |
| author_facet | Chan-Hee Kim Eun Jeong Kim Chaehwa Seo Yoon Kwon Nam |
| author_sort | Chan-Hee Kim |
| collection | DOAJ |
| description | Maternal genes are important in directing early development and determining egg quality in fish. We here report the de novo transcriptome from four tissue libraries of the cyprinid loach, <i>Misgurnus anguillicaudatus</i>, and for the first time identified maternal gene transcripts in unfertilized eggs and suggest their immune system involvement. Expression profiles and functional enrichment revealed a total 24,116 transcripts were expressed as maternal transcripts in unfertilized eggs, which were involved in a wide range of biological functions and pathways. Comparison expression profiles and analysis of tissue specificity revealed that the large numbers of maternal transcripts were stored in unfertilized eggs near the late phase of ovarian maturation and before ovulation. Functional classification showed a total of 279 maternal immune-related transcripts classified with immune system process GO term and immune system KEGG pathway. qPCR analysis showed that transcript levels of identified maternal immune-related candidate genes were dynamically modulated during development and early ontogeny of <i>M. anguillicaudatus</i>. Taken together, this study could not only provide knowledge on the protective roles of maternal immune-related genes during early life stage of <i>M. anguillicaudatus</i> but could also be a valuable transcriptomic/genomic resource for further analysis of maternally provisioned genes in <i>M. anguillicaudatus</i> and other related teleost fishes. |
| first_indexed | 2024-03-10T19:30:23Z |
| format | Article |
| id | doaj.art-25658297e67544a0aa9b535adba5440b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T19:30:23Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-25658297e67544a0aa9b535adba5440b2023-11-20T02:09:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-05-012111387210.3390/ijms21113872Transcriptome Analysis of Maternal Gene Transcripts in Unfertilized Eggs of <i>Misgurnus anguillicaudatus</i> and Identification of Immune-Related Maternal GenesChan-Hee Kim0Eun Jeong Kim1Chaehwa Seo2Yoon Kwon Nam3Department of Marine Bio-Materials and Aquaculture, College of Fisheries Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, KoreaDepartment of Marine Bio-Materials and Aquaculture, College of Fisheries Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, KoreaBioinformatics Team, DNA Link Inc., 150 Bugahyeon-ro, Seodaemun-gu, Seoul 03759, KoreaDepartment of Marine Bio-Materials and Aquaculture, College of Fisheries Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, KoreaMaternal genes are important in directing early development and determining egg quality in fish. We here report the de novo transcriptome from four tissue libraries of the cyprinid loach, <i>Misgurnus anguillicaudatus</i>, and for the first time identified maternal gene transcripts in unfertilized eggs and suggest their immune system involvement. Expression profiles and functional enrichment revealed a total 24,116 transcripts were expressed as maternal transcripts in unfertilized eggs, which were involved in a wide range of biological functions and pathways. Comparison expression profiles and analysis of tissue specificity revealed that the large numbers of maternal transcripts were stored in unfertilized eggs near the late phase of ovarian maturation and before ovulation. Functional classification showed a total of 279 maternal immune-related transcripts classified with immune system process GO term and immune system KEGG pathway. qPCR analysis showed that transcript levels of identified maternal immune-related candidate genes were dynamically modulated during development and early ontogeny of <i>M. anguillicaudatus</i>. Taken together, this study could not only provide knowledge on the protective roles of maternal immune-related genes during early life stage of <i>M. anguillicaudatus</i> but could also be a valuable transcriptomic/genomic resource for further analysis of maternally provisioned genes in <i>M. anguillicaudatus</i> and other related teleost fishes.https://www.mdpi.com/1422-0067/21/11/3872de novo transcriptomematernal gene transcriptunfertilized eggimmune-related gene<i>Misgurnus anguillicaudatus</i> |
| spellingShingle | Chan-Hee Kim Eun Jeong Kim Chaehwa Seo Yoon Kwon Nam Transcriptome Analysis of Maternal Gene Transcripts in Unfertilized Eggs of <i>Misgurnus anguillicaudatus</i> and Identification of Immune-Related Maternal Genes International Journal of Molecular Sciences de novo transcriptome maternal gene transcript unfertilized egg immune-related gene <i>Misgurnus anguillicaudatus</i> |
| title | Transcriptome Analysis of Maternal Gene Transcripts in Unfertilized Eggs of <i>Misgurnus anguillicaudatus</i> and Identification of Immune-Related Maternal Genes |
| title_full | Transcriptome Analysis of Maternal Gene Transcripts in Unfertilized Eggs of <i>Misgurnus anguillicaudatus</i> and Identification of Immune-Related Maternal Genes |
| title_fullStr | Transcriptome Analysis of Maternal Gene Transcripts in Unfertilized Eggs of <i>Misgurnus anguillicaudatus</i> and Identification of Immune-Related Maternal Genes |
| title_full_unstemmed | Transcriptome Analysis of Maternal Gene Transcripts in Unfertilized Eggs of <i>Misgurnus anguillicaudatus</i> and Identification of Immune-Related Maternal Genes |
| title_short | Transcriptome Analysis of Maternal Gene Transcripts in Unfertilized Eggs of <i>Misgurnus anguillicaudatus</i> and Identification of Immune-Related Maternal Genes |
| title_sort | transcriptome analysis of maternal gene transcripts in unfertilized eggs of i misgurnus anguillicaudatus i and identification of immune related maternal genes |
| topic | de novo transcriptome maternal gene transcript unfertilized egg immune-related gene <i>Misgurnus anguillicaudatus</i> |
| url | https://www.mdpi.com/1422-0067/21/11/3872 |
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