Chaperonin TRiC/CCT subunit CCT7 is involved in the replication of canine parvovirus in F81 cells
Canine parvovirus (CPV) is one of the most common lethal viruses in canines. The virus disease is prevalent throughout the year, with high morbidity and mortality rate, causing serious harm to dogs and the dog industry. Previously, yeast two hybrid method was used to screen the protein chaperonin co...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1346894/full |
| _version_ | 1797322338916630528 |
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| author | Xia Su Hongzhuan Zhou Fuzhou Xu Jin Zhang Bing Xiao Qi Qi Lulu Lin Bing Yang |
| author_facet | Xia Su Hongzhuan Zhou Fuzhou Xu Jin Zhang Bing Xiao Qi Qi Lulu Lin Bing Yang |
| author_sort | Xia Su |
| collection | DOAJ |
| description | Canine parvovirus (CPV) is one of the most common lethal viruses in canines. The virus disease is prevalent throughout the year, with high morbidity and mortality rate, causing serious harm to dogs and the dog industry. Previously, yeast two hybrid method was used to screen the protein chaperonin containing TCP-1 (CCT7) that interacts with VP2. However, the mechanism of interactions between CCT7 and VP2 on CPV replication remains unclear. In this study, we first verified the interaction between CCT7 and viral VP2 proteins using yeast one-to-one experiment and co-immunoprecipitation (CoIP) experiment. Laser confocal microscopy observation showed that CCT7 and VP2 were able to co-localize and were mostly localized in the cytoplasm. In addition, the study of VP2 truncated mutant found that the interaction region of VP2 with CCT7 was located between amino acids 231 and 320. Cycloheximide (CHX) chase experiments showed that CCT7 can improve the stability of VP2 protein. After further regulation of CCT7 expression in F81 cells, it was found that the expression level of VP2 protein was significantly reduced after knocking down CCT7 expression by RNA interference (RNAi) or HSF1A inhibitor, and increased after overexpressing host CCT7. The study reveals the role of VP2 interacting protein CCT7 in the replication process of CPV, which could provide a potential target for the prevention and control of CPV. |
| first_indexed | 2024-03-08T05:11:51Z |
| format | Article |
| id | doaj.art-2565d7e5d3c040f79949d5226fee4545 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T05:11:51Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2565d7e5d3c040f79949d5226fee45452024-02-07T05:34:29ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-02-011510.3389/fmicb.2024.13468941346894Chaperonin TRiC/CCT subunit CCT7 is involved in the replication of canine parvovirus in F81 cellsXia SuHongzhuan ZhouFuzhou XuJin ZhangBing XiaoQi QiLulu LinBing YangCanine parvovirus (CPV) is one of the most common lethal viruses in canines. The virus disease is prevalent throughout the year, with high morbidity and mortality rate, causing serious harm to dogs and the dog industry. Previously, yeast two hybrid method was used to screen the protein chaperonin containing TCP-1 (CCT7) that interacts with VP2. However, the mechanism of interactions between CCT7 and VP2 on CPV replication remains unclear. In this study, we first verified the interaction between CCT7 and viral VP2 proteins using yeast one-to-one experiment and co-immunoprecipitation (CoIP) experiment. Laser confocal microscopy observation showed that CCT7 and VP2 were able to co-localize and were mostly localized in the cytoplasm. In addition, the study of VP2 truncated mutant found that the interaction region of VP2 with CCT7 was located between amino acids 231 and 320. Cycloheximide (CHX) chase experiments showed that CCT7 can improve the stability of VP2 protein. After further regulation of CCT7 expression in F81 cells, it was found that the expression level of VP2 protein was significantly reduced after knocking down CCT7 expression by RNA interference (RNAi) or HSF1A inhibitor, and increased after overexpressing host CCT7. The study reveals the role of VP2 interacting protein CCT7 in the replication process of CPV, which could provide a potential target for the prevention and control of CPV.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1346894/fullcanine parvovirusCCT7VP2replicationstability |
| spellingShingle | Xia Su Hongzhuan Zhou Fuzhou Xu Jin Zhang Bing Xiao Qi Qi Lulu Lin Bing Yang Chaperonin TRiC/CCT subunit CCT7 is involved in the replication of canine parvovirus in F81 cells Frontiers in Microbiology canine parvovirus CCT7 VP2 replication stability |
| title | Chaperonin TRiC/CCT subunit CCT7 is involved in the replication of canine parvovirus in F81 cells |
| title_full | Chaperonin TRiC/CCT subunit CCT7 is involved in the replication of canine parvovirus in F81 cells |
| title_fullStr | Chaperonin TRiC/CCT subunit CCT7 is involved in the replication of canine parvovirus in F81 cells |
| title_full_unstemmed | Chaperonin TRiC/CCT subunit CCT7 is involved in the replication of canine parvovirus in F81 cells |
| title_short | Chaperonin TRiC/CCT subunit CCT7 is involved in the replication of canine parvovirus in F81 cells |
| title_sort | chaperonin tric cct subunit cct7 is involved in the replication of canine parvovirus in f81 cells |
| topic | canine parvovirus CCT7 VP2 replication stability |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1346894/full |
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