Possible bioremediation of arsenic toxicity by isolating indigenous bacteria from the middle Gangetic plain of Bihar, India
In middle Gangetic plain, high arsenic concentration is present in water, which causes a significant health risk. Total 48 morphologically distinct arsenite resistant bacteria were isolated from middle Gangetic plain. The minimum inhibitory concentration (MIC) values of arsenite varied widely in the...
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| Format: | Article |
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Elsevier
2018-03-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X17300899 |
| _version_ | 1811199967183765504 |
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| author | Ghanshyam Kumar Satyapal Santosh Kumar Mishra Amrita Srivastava Rajesh Kumar Ranjan Krishna Prakash Rizwanul Haque Nitish Kumar |
| author_facet | Ghanshyam Kumar Satyapal Santosh Kumar Mishra Amrita Srivastava Rajesh Kumar Ranjan Krishna Prakash Rizwanul Haque Nitish Kumar |
| author_sort | Ghanshyam Kumar Satyapal |
| collection | DOAJ |
| description | In middle Gangetic plain, high arsenic concentration is present in water, which causes a significant health risk. Total 48 morphologically distinct arsenite resistant bacteria were isolated from middle Gangetic plain. The minimum inhibitory concentration (MIC) values of arsenite varied widely in the range 1–15 mM of the isolates. On the basis of their MIC, two isolates, AK1 (KY569423) and AK9 (KY569424) were selected. The analysis of the 16S rRNA gene sequence of selected isolates revealed that they are belong to the genus Pseudomonas. The AgNO3 test based microplate method revealed that isolates, AK1 and AK9, have potential in transformation of arsenic species. Further, the presence of aoxR, aoxB and aoxC genes in the both isolated strain AK1 and AK9 was confirmed, which play an important role in arsenic bioremediation by arsenite oxidation. Isolated strains also showed heavy metal resistance against Cr(IV), Ni(II), Co(II), Pb(II), Cu(II), Hg(II), Ag(I) and Cd(II). |
| first_indexed | 2024-04-12T01:56:43Z |
| format | Article |
| id | doaj.art-25664a3b24484bbd8b72ad0d735260af |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-12T01:56:43Z |
| publishDate | 2018-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-25664a3b24484bbd8b72ad0d735260af2022-12-22T03:52:47ZengElsevierBiotechnology Reports2215-017X2018-03-0117C11712510.1016/j.btre.2018.02.002Possible bioremediation of arsenic toxicity by isolating indigenous bacteria from the middle Gangetic plain of Bihar, IndiaGhanshyam Kumar Satyapal0Santosh Kumar Mishra1Amrita Srivastava2Rajesh Kumar Ranjan3Krishna Prakash4Rizwanul Haque5Nitish Kumar6Centre for Biological Sciences (Biotechnology), Central University of South Bihar, Patna, Bihar, IndiaCentre for Biological Sciences (Biotechnology), Central University of South Bihar, Patna, Bihar, IndiaCentre for Biological Sciences (Life Science), Central University of South Bihar, Patna, Bihar, IndiaCentre for Environmental Sciences, Central University of South Bihar, Patna, Bihar, IndiaCentre for Biological Sciences (Biotechnology), Central University of South Bihar, Patna, Bihar, IndiaCentre for Biological Sciences (Biotechnology), Central University of South Bihar, Patna, Bihar, IndiaCentre for Biological Sciences (Biotechnology), Central University of South Bihar, Patna, Bihar, IndiaIn middle Gangetic plain, high arsenic concentration is present in water, which causes a significant health risk. Total 48 morphologically distinct arsenite resistant bacteria were isolated from middle Gangetic plain. The minimum inhibitory concentration (MIC) values of arsenite varied widely in the range 1–15 mM of the isolates. On the basis of their MIC, two isolates, AK1 (KY569423) and AK9 (KY569424) were selected. The analysis of the 16S rRNA gene sequence of selected isolates revealed that they are belong to the genus Pseudomonas. The AgNO3 test based microplate method revealed that isolates, AK1 and AK9, have potential in transformation of arsenic species. Further, the presence of aoxR, aoxB and aoxC genes in the both isolated strain AK1 and AK9 was confirmed, which play an important role in arsenic bioremediation by arsenite oxidation. Isolated strains also showed heavy metal resistance against Cr(IV), Ni(II), Co(II), Pb(II), Cu(II), Hg(II), Ag(I) and Cd(II).http://www.sciencedirect.com/science/article/pii/S2215017X17300899ArsenicBacteriaBioremediationMiddle Gangetic plainOxidation |
| spellingShingle | Ghanshyam Kumar Satyapal Santosh Kumar Mishra Amrita Srivastava Rajesh Kumar Ranjan Krishna Prakash Rizwanul Haque Nitish Kumar Possible bioremediation of arsenic toxicity by isolating indigenous bacteria from the middle Gangetic plain of Bihar, India Biotechnology Reports Arsenic Bacteria Bioremediation Middle Gangetic plain Oxidation |
| title | Possible bioremediation of arsenic toxicity by isolating indigenous bacteria from the middle Gangetic plain of Bihar, India |
| title_full | Possible bioremediation of arsenic toxicity by isolating indigenous bacteria from the middle Gangetic plain of Bihar, India |
| title_fullStr | Possible bioremediation of arsenic toxicity by isolating indigenous bacteria from the middle Gangetic plain of Bihar, India |
| title_full_unstemmed | Possible bioremediation of arsenic toxicity by isolating indigenous bacteria from the middle Gangetic plain of Bihar, India |
| title_short | Possible bioremediation of arsenic toxicity by isolating indigenous bacteria from the middle Gangetic plain of Bihar, India |
| title_sort | possible bioremediation of arsenic toxicity by isolating indigenous bacteria from the middle gangetic plain of bihar india |
| topic | Arsenic Bacteria Bioremediation Middle Gangetic plain Oxidation |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X17300899 |
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