Highly Sensitive Nanomagnetic Quantification of Extracellular Vesicles by Immunochromatographic Strips: A Tool for Liquid Biopsy
Extracellular vesicles (EVs) are promising agents for liquid biopsy—a non-invasive approach for the diagnosis of cancer and evaluation of therapy response. However, EV potential is limited by the lack of sufficiently sensitive, time-, and cost-efficient methods for their registration. This research...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/9/1579 |
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| author | Vera A. Bragina Elena Khomyakova Alexey V. Orlov Sergey L. Znoyko Elizaveta N. Mochalova Liliia Paniushkina Victoria O. Shender Thalia Erbes Evgeniy G. Evtushenko Dmitry V. Bagrov Victoria N. Lavrenova Irina Nazarenko Petr I. Nikitin |
| author_facet | Vera A. Bragina Elena Khomyakova Alexey V. Orlov Sergey L. Znoyko Elizaveta N. Mochalova Liliia Paniushkina Victoria O. Shender Thalia Erbes Evgeniy G. Evtushenko Dmitry V. Bagrov Victoria N. Lavrenova Irina Nazarenko Petr I. Nikitin |
| author_sort | Vera A. Bragina |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) are promising agents for liquid biopsy—a non-invasive approach for the diagnosis of cancer and evaluation of therapy response. However, EV potential is limited by the lack of sufficiently sensitive, time-, and cost-efficient methods for their registration. This research aimed at developing a highly sensitive and easy-to-use immunochromatographic tool based on magnetic nanoparticles for EV quantification. The tool is demonstrated by detection of EVs isolated from cell culture supernatants and various body fluids using characteristic biomarkers, CD9 and CD81, and a tumor-associated marker—epithelial cell adhesion molecules. The detection limit of 3.7 × 10<sup>5</sup> EV/µL is one to two orders better than the most sensitive traditional lateral flow system and commercial ELISA kits. The detection specificity is ensured by an isotype control line on the test strip. The tool’s advantages are due to the spatial quantification of EV-bound magnetic nanolabels within the strip volume by an original electronic technique. The inexpensive tool, promising for liquid biopsy in daily clinical routines, can be extended to other relevant biomarkers. |
| first_indexed | 2024-03-10T03:51:27Z |
| format | Article |
| id | doaj.art-25669832ab2a4e998ad7af22dfab4a88 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T03:51:27Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-25669832ab2a4e998ad7af22dfab4a882023-11-23T08:56:24ZengMDPI AGNanomaterials2079-49912022-05-01129157910.3390/nano12091579Highly Sensitive Nanomagnetic Quantification of Extracellular Vesicles by Immunochromatographic Strips: A Tool for Liquid BiopsyVera A. Bragina0Elena Khomyakova1Alexey V. Orlov2Sergey L. Znoyko3Elizaveta N. Mochalova4Liliia Paniushkina5Victoria O. Shender6Thalia Erbes7Evgeniy G. Evtushenko8Dmitry V. Bagrov9Victoria N. Lavrenova10Irina Nazarenko11Petr I. Nikitin12Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., 119991 Moscow, RussiaInstitute for Infection Prevention and Hospital Epidemiology, Medical Center—University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, GermanyFederal Research and Clinical Center of Physical-Chemical Medicine of the Federal Medical and Biological Agency, 1a Malaya Pirogovskaya St., 119992 Moscow, RussiaDepartment of Obstetrics and Gynecology, Medical Center—University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, GermanyLomonosov Moscow State University, 1 Leninskie Gory, 119991 Moscow, RussiaLomonosov Moscow State University, 1 Leninskie Gory, 119991 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of the Federal Medical and Biological Agency, 1a Malaya Pirogovskaya St., 119992 Moscow, RussiaInstitute for Infection Prevention and Hospital Epidemiology, Medical Center—University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, GermanyProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., 119991 Moscow, RussiaExtracellular vesicles (EVs) are promising agents for liquid biopsy—a non-invasive approach for the diagnosis of cancer and evaluation of therapy response. However, EV potential is limited by the lack of sufficiently sensitive, time-, and cost-efficient methods for their registration. This research aimed at developing a highly sensitive and easy-to-use immunochromatographic tool based on magnetic nanoparticles for EV quantification. The tool is demonstrated by detection of EVs isolated from cell culture supernatants and various body fluids using characteristic biomarkers, CD9 and CD81, and a tumor-associated marker—epithelial cell adhesion molecules. The detection limit of 3.7 × 10<sup>5</sup> EV/µL is one to two orders better than the most sensitive traditional lateral flow system and commercial ELISA kits. The detection specificity is ensured by an isotype control line on the test strip. The tool’s advantages are due to the spatial quantification of EV-bound magnetic nanolabels within the strip volume by an original electronic technique. The inexpensive tool, promising for liquid biopsy in daily clinical routines, can be extended to other relevant biomarkers.https://www.mdpi.com/2079-4991/12/9/1579antibody-functionalized magnetic nanoparticlesmagnetic particle quantificationnonlinear magnetizationimmunochromatographic test stripsextracellular vesiclesbreast and ovarian cancers |
| spellingShingle | Vera A. Bragina Elena Khomyakova Alexey V. Orlov Sergey L. Znoyko Elizaveta N. Mochalova Liliia Paniushkina Victoria O. Shender Thalia Erbes Evgeniy G. Evtushenko Dmitry V. Bagrov Victoria N. Lavrenova Irina Nazarenko Petr I. Nikitin Highly Sensitive Nanomagnetic Quantification of Extracellular Vesicles by Immunochromatographic Strips: A Tool for Liquid Biopsy Nanomaterials antibody-functionalized magnetic nanoparticles magnetic particle quantification nonlinear magnetization immunochromatographic test strips extracellular vesicles breast and ovarian cancers |
| title | Highly Sensitive Nanomagnetic Quantification of Extracellular Vesicles by Immunochromatographic Strips: A Tool for Liquid Biopsy |
| title_full | Highly Sensitive Nanomagnetic Quantification of Extracellular Vesicles by Immunochromatographic Strips: A Tool for Liquid Biopsy |
| title_fullStr | Highly Sensitive Nanomagnetic Quantification of Extracellular Vesicles by Immunochromatographic Strips: A Tool for Liquid Biopsy |
| title_full_unstemmed | Highly Sensitive Nanomagnetic Quantification of Extracellular Vesicles by Immunochromatographic Strips: A Tool for Liquid Biopsy |
| title_short | Highly Sensitive Nanomagnetic Quantification of Extracellular Vesicles by Immunochromatographic Strips: A Tool for Liquid Biopsy |
| title_sort | highly sensitive nanomagnetic quantification of extracellular vesicles by immunochromatographic strips a tool for liquid biopsy |
| topic | antibody-functionalized magnetic nanoparticles magnetic particle quantification nonlinear magnetization immunochromatographic test strips extracellular vesicles breast and ovarian cancers |
| url | https://www.mdpi.com/2079-4991/12/9/1579 |
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