Effectors of the Type VI Secretion System Have the Potential to Be Modified into Antimicrobial Peptides
ABSTRACT The use of antibiotics has led to the emergence of multidrug-resistant (MDR) bacteria, and there is an urgent need to find alternative treatments to alleviate this pressure. The type VI secretion system (T6SS) is a protein delivery system present in bacterial cells that secretes effectors t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-08-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.00308-23 |
| _version_ | 1797741745449992192 |
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| author | Wenjia Lu Hao Lu Chenchen Wang Gaoyan Wang Wenqi Dong Chen Tan |
| author_facet | Wenjia Lu Hao Lu Chenchen Wang Gaoyan Wang Wenqi Dong Chen Tan |
| author_sort | Wenjia Lu |
| collection | DOAJ |
| description | ABSTRACT The use of antibiotics has led to the emergence of multidrug-resistant (MDR) bacteria, and there is an urgent need to find alternative treatments to alleviate this pressure. The type VI secretion system (T6SS) is a protein delivery system present in bacterial cells that secretes effectors that participate in bacterial virulence. Given the potential for the transformation of these effectors into antimicrobial peptides (AMPs), we designed T6SS effectors into AMPs that have a membrane-disrupting effect. These effectors kill bacteria by altering the membrane potential and increasing the intracellular reactive oxygen species (ROS) content. Moreover, AMPs also have a significant therapeutic effect both in vivo and in vitro. This finding suggests that it is possible to modify bacterial components of bacteria themselves to create compounds that fight bacteria. IMPORTANCE This study first identified and modified the T6SS effector into positively charged alpha-helical peptides. These peptides have good antibacterial and bactericidal effects on G+ bacteria and G− bacteria. This study broadens the source of AMPs and makes T6SS effectors more useful. |
| first_indexed | 2024-03-12T14:31:05Z |
| format | Article |
| id | doaj.art-2567d4df39734378a4fbbd4975105773 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-12T14:31:05Z |
| publishDate | 2023-08-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-2567d4df39734378a4fbbd49751057732023-08-17T13:04:15ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-08-0111410.1128/spectrum.00308-23Effectors of the Type VI Secretion System Have the Potential to Be Modified into Antimicrobial PeptidesWenjia Lu0Hao Lu1Chenchen Wang2Gaoyan Wang3Wenqi Dong4Chen Tan5National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, ChinaABSTRACT The use of antibiotics has led to the emergence of multidrug-resistant (MDR) bacteria, and there is an urgent need to find alternative treatments to alleviate this pressure. The type VI secretion system (T6SS) is a protein delivery system present in bacterial cells that secretes effectors that participate in bacterial virulence. Given the potential for the transformation of these effectors into antimicrobial peptides (AMPs), we designed T6SS effectors into AMPs that have a membrane-disrupting effect. These effectors kill bacteria by altering the membrane potential and increasing the intracellular reactive oxygen species (ROS) content. Moreover, AMPs also have a significant therapeutic effect both in vivo and in vitro. This finding suggests that it is possible to modify bacterial components of bacteria themselves to create compounds that fight bacteria. IMPORTANCE This study first identified and modified the T6SS effector into positively charged alpha-helical peptides. These peptides have good antibacterial and bactericidal effects on G+ bacteria and G− bacteria. This study broadens the source of AMPs and makes T6SS effectors more useful.https://journals.asm.org/doi/10.1128/spectrum.00308-23T6SS effectorantimicrobial peptidesmultidrug-resistantTsapanti-inflammation |
| spellingShingle | Wenjia Lu Hao Lu Chenchen Wang Gaoyan Wang Wenqi Dong Chen Tan Effectors of the Type VI Secretion System Have the Potential to Be Modified into Antimicrobial Peptides Microbiology Spectrum T6SS effector antimicrobial peptides multidrug-resistant Tsap anti-inflammation |
| title | Effectors of the Type VI Secretion System Have the Potential to Be Modified into Antimicrobial Peptides |
| title_full | Effectors of the Type VI Secretion System Have the Potential to Be Modified into Antimicrobial Peptides |
| title_fullStr | Effectors of the Type VI Secretion System Have the Potential to Be Modified into Antimicrobial Peptides |
| title_full_unstemmed | Effectors of the Type VI Secretion System Have the Potential to Be Modified into Antimicrobial Peptides |
| title_short | Effectors of the Type VI Secretion System Have the Potential to Be Modified into Antimicrobial Peptides |
| title_sort | effectors of the type vi secretion system have the potential to be modified into antimicrobial peptides |
| topic | T6SS effector antimicrobial peptides multidrug-resistant Tsap anti-inflammation |
| url | https://journals.asm.org/doi/10.1128/spectrum.00308-23 |
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