Phenotypic and Genotypic Antimicrobial Resistance in Non-O157 Shiga Toxin-Producing Escherichia coli Isolated From Cattle and Swine in Chile
Non-O157 Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen that causes bloody diarrhea and hemolytic-uremic syndrome in humans, and a major cause of foodborne disease. Despite antibiotic treatment of STEC infections in humans is not recommended, the presence of antimicrobial-resis...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fvets.2020.00367/full |
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| author | Nicolás Galarce Fernando Sánchez Verónica Fuenzalida Romina Ramos Beatriz Escobar Lisette Lapierre Esteban Paredes-Osses Gabriel Arriagada Raúl Alegría-Morán Raúl Alegría-Morán Nilton Lincopán Danny Fuentes-Castillo Alejandra Vera-Leiva Gerardo González-Rocha Gerardo González-Rocha Helia Bello-Toledo Helia Bello-Toledo Consuelo Borie |
| author_facet | Nicolás Galarce Fernando Sánchez Verónica Fuenzalida Romina Ramos Beatriz Escobar Lisette Lapierre Esteban Paredes-Osses Gabriel Arriagada Raúl Alegría-Morán Raúl Alegría-Morán Nilton Lincopán Danny Fuentes-Castillo Alejandra Vera-Leiva Gerardo González-Rocha Gerardo González-Rocha Helia Bello-Toledo Helia Bello-Toledo Consuelo Borie |
| author_sort | Nicolás Galarce |
| collection | DOAJ |
| description | Non-O157 Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen that causes bloody diarrhea and hemolytic-uremic syndrome in humans, and a major cause of foodborne disease. Despite antibiotic treatment of STEC infections in humans is not recommended, the presence of antimicrobial-resistant bacteria in animals and food constitutes a risk to public health, as the pool of genes from which pathogenic bacteria can acquire antibiotic resistance has increased. Additionally, in Chile there is no information on the antimicrobial resistance of this pathogen in livestock. Thus, the aim of this study was to characterize the phenotypic and genotypic antimicrobial resistance of STEC strains isolated from cattle and swine in the Metropolitan region, Chile, to contribute relevant data to antimicrobial resistance surveillance programs at national and international level. We assessed the minimal inhibitory concentration of 18 antimicrobials, and the distribution of 12 antimicrobial resistance genes and class 1 and 2 integrons in 54 STEC strains. All strains were phenotypically resistant to at least one antimicrobial drug, with a 100% of resistance to cefalexin, followed by colistin (81.5%), chloramphenicol (14.8%), ampicillin and enrofloxacin (5.6% each), doxycycline (3.7%), and cefovecin (1.9%). Most detected antibiotic resistance genes were dfrA1 and tetA (100%), followed by tetB (94.4%), blaTEM−1 (90.7%), aac(6)-Ib (88.9%), blaAmpC (81.5%), cat1 (61.1%), and aac(3)-IIa (11.1%). Integrons were detected only in strains of swine origin. Therefore, this study provides further evidence that non-O157 STEC strains present in livestock in the Metropolitan region of Chile exhibit phenotypic and genotypic resistance against antimicrobials that are critical for human and veterinary medicine, representing a major threat for public health. Additionally, these strains could have a competitive advantage in the presence of antimicrobial selective pressure, leading to an increase in food contamination. This study highlights the need for coordinated local and global actions regarding the use of antimicrobials in animal food production. |
| first_indexed | 2024-12-12T19:53:26Z |
| format | Article |
| id | doaj.art-256801589a3b42c8801789f43d1c3e9f |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-12T19:53:26Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-256801589a3b42c8801789f43d1c3e9f2022-12-22T00:13:55ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-07-01710.3389/fvets.2020.00367538595Phenotypic and Genotypic Antimicrobial Resistance in Non-O157 Shiga Toxin-Producing Escherichia coli Isolated From Cattle and Swine in ChileNicolás Galarce0Fernando Sánchez1Verónica Fuenzalida2Romina Ramos3Beatriz Escobar4Lisette Lapierre5Esteban Paredes-Osses6Gabriel Arriagada7Raúl Alegría-Morán8Raúl Alegría-Morán9Nilton Lincopán10Danny Fuentes-Castillo11Alejandra Vera-Leiva12Gerardo González-Rocha13Gerardo González-Rocha14Helia Bello-Toledo15Helia Bello-Toledo16Consuelo Borie17Departamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileDepartamento de Salud Ambiental, Instituto de Salud Pública de Chile, Santiago, ChileInstituto de Ciencias Agroalimentarias, Animales y Ambientales, Universidad de O'Higgins, San Fernando, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileFacultad de Ciencias Agropecuarias, Universidad Pedro de Valdivia, Santiago, ChileDepartamento de Microbiología, Instituto de Ciências Biomedicas, Universidade de São Paulo, São Paulo, BrazilDepartamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, BrazilLaboratorio de Investigación en Agentes Antibacterianos, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileLaboratorio de Investigación en Agentes Antibacterianos, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileMillenium Nucleus on Interdisciplinary Approach to Antimicrobial Resistance, Santiago, ChileLaboratorio de Investigación en Agentes Antibacterianos, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, ChileMillenium Nucleus on Interdisciplinary Approach to Antimicrobial Resistance, Santiago, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, ChileNon-O157 Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen that causes bloody diarrhea and hemolytic-uremic syndrome in humans, and a major cause of foodborne disease. Despite antibiotic treatment of STEC infections in humans is not recommended, the presence of antimicrobial-resistant bacteria in animals and food constitutes a risk to public health, as the pool of genes from which pathogenic bacteria can acquire antibiotic resistance has increased. Additionally, in Chile there is no information on the antimicrobial resistance of this pathogen in livestock. Thus, the aim of this study was to characterize the phenotypic and genotypic antimicrobial resistance of STEC strains isolated from cattle and swine in the Metropolitan region, Chile, to contribute relevant data to antimicrobial resistance surveillance programs at national and international level. We assessed the minimal inhibitory concentration of 18 antimicrobials, and the distribution of 12 antimicrobial resistance genes and class 1 and 2 integrons in 54 STEC strains. All strains were phenotypically resistant to at least one antimicrobial drug, with a 100% of resistance to cefalexin, followed by colistin (81.5%), chloramphenicol (14.8%), ampicillin and enrofloxacin (5.6% each), doxycycline (3.7%), and cefovecin (1.9%). Most detected antibiotic resistance genes were dfrA1 and tetA (100%), followed by tetB (94.4%), blaTEM−1 (90.7%), aac(6)-Ib (88.9%), blaAmpC (81.5%), cat1 (61.1%), and aac(3)-IIa (11.1%). Integrons were detected only in strains of swine origin. Therefore, this study provides further evidence that non-O157 STEC strains present in livestock in the Metropolitan region of Chile exhibit phenotypic and genotypic resistance against antimicrobials that are critical for human and veterinary medicine, representing a major threat for public health. Additionally, these strains could have a competitive advantage in the presence of antimicrobial selective pressure, leading to an increase in food contamination. This study highlights the need for coordinated local and global actions regarding the use of antimicrobials in animal food production.https://www.frontiersin.org/article/10.3389/fvets.2020.00367/fullantimicrobial resistanceShiga toxinEscherichia colidrug resistancecattleswine |
| spellingShingle | Nicolás Galarce Fernando Sánchez Verónica Fuenzalida Romina Ramos Beatriz Escobar Lisette Lapierre Esteban Paredes-Osses Gabriel Arriagada Raúl Alegría-Morán Raúl Alegría-Morán Nilton Lincopán Danny Fuentes-Castillo Alejandra Vera-Leiva Gerardo González-Rocha Gerardo González-Rocha Helia Bello-Toledo Helia Bello-Toledo Consuelo Borie Phenotypic and Genotypic Antimicrobial Resistance in Non-O157 Shiga Toxin-Producing Escherichia coli Isolated From Cattle and Swine in Chile Frontiers in Veterinary Science antimicrobial resistance Shiga toxin Escherichia coli drug resistance cattle swine |
| title | Phenotypic and Genotypic Antimicrobial Resistance in Non-O157 Shiga Toxin-Producing Escherichia coli Isolated From Cattle and Swine in Chile |
| title_full | Phenotypic and Genotypic Antimicrobial Resistance in Non-O157 Shiga Toxin-Producing Escherichia coli Isolated From Cattle and Swine in Chile |
| title_fullStr | Phenotypic and Genotypic Antimicrobial Resistance in Non-O157 Shiga Toxin-Producing Escherichia coli Isolated From Cattle and Swine in Chile |
| title_full_unstemmed | Phenotypic and Genotypic Antimicrobial Resistance in Non-O157 Shiga Toxin-Producing Escherichia coli Isolated From Cattle and Swine in Chile |
| title_short | Phenotypic and Genotypic Antimicrobial Resistance in Non-O157 Shiga Toxin-Producing Escherichia coli Isolated From Cattle and Swine in Chile |
| title_sort | phenotypic and genotypic antimicrobial resistance in non o157 shiga toxin producing escherichia coli isolated from cattle and swine in chile |
| topic | antimicrobial resistance Shiga toxin Escherichia coli drug resistance cattle swine |
| url | https://www.frontiersin.org/article/10.3389/fvets.2020.00367/full |
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