Exploration of unique relation among industrial fungi by statistical analysis
This work was carried out to explore the relation among thermophilic cellulolytic fungi, which are of industrialimportance. There was no report found about the genetic relationship of fungi, which are used to produce industrial enzymes.So the aim of the study was to observe the simil...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2012-12-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://rdo.psu.ac.th/sjstweb/journal/34-6/0597-0721-34-6-607-613.pdf |
| _version_ | 1818309282708324352 |
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| author | Asma Siddique Aftab Bashir Farooq Latif |
| author_facet | Asma Siddique Aftab Bashir Farooq Latif |
| author_sort | Asma Siddique |
| collection | DOAJ |
| description | This work was carried out to explore the relation among thermophilic cellulolytic fungi, which are of industrialimportance. There was no report found about the genetic relationship of fungi, which are used to produce industrial enzymes.So the aim of the study was to observe the similarity among different cellulolytic fungi on genetic level, which will providethe background to understand the correlation among cellulase producing systems of these fungi. Eleven (11) fungi werestudied for genetic diversity using the Random Amplified Polymorphic DNA (RAPD) a PCR based molecular marker system.In this regard twenty universal decamers used for RAPD resulted in 1527 numbers of bands observed during comparison ofall wild strains. Maximum polymorphism was generated with GLA-07. Average numbers of bands per 20 primers were 65-72.An Interesting feature of the study was the similarity of Humicola insolens with Torula thermophile, more than with theother members of the Humicola family. This genetic pattern affects the physical structure of the fungi. Spores of Torulathermophila are more related to Humicola insolens than to its own family. Similarity between the two was found to be 57.8%,whereas between Humicola lanuginosa (Thermomysis lanuginosus) and Humicola grisea it was 57.3%. Apart from this,similarity between Talaromyces dupontii and Rhizomucor pusillus was 51.5%. Least similarity was found in Rhizomucorpusillus and Humicola grisea, which was 18.7% and Chaetomium thermophile and Sporotrichum thermophile, which was18.3%. Genetic similarity matrix was constructed on the basis of Nei and Li’s index. |
| first_indexed | 2024-12-13T07:27:42Z |
| format | Article |
| id | doaj.art-256be203f1484bdca3e59fd878c86ffa |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-13T07:27:42Z |
| publishDate | 2012-12-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-256be203f1484bdca3e59fd878c86ffa2022-12-21T23:55:16ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952012-12-01346607613Exploration of unique relation among industrial fungi by statistical analysisAsma SiddiqueAftab BashirFarooq LatifThis work was carried out to explore the relation among thermophilic cellulolytic fungi, which are of industrialimportance. There was no report found about the genetic relationship of fungi, which are used to produce industrial enzymes.So the aim of the study was to observe the similarity among different cellulolytic fungi on genetic level, which will providethe background to understand the correlation among cellulase producing systems of these fungi. Eleven (11) fungi werestudied for genetic diversity using the Random Amplified Polymorphic DNA (RAPD) a PCR based molecular marker system.In this regard twenty universal decamers used for RAPD resulted in 1527 numbers of bands observed during comparison ofall wild strains. Maximum polymorphism was generated with GLA-07. Average numbers of bands per 20 primers were 65-72.An Interesting feature of the study was the similarity of Humicola insolens with Torula thermophile, more than with theother members of the Humicola family. This genetic pattern affects the physical structure of the fungi. Spores of Torulathermophila are more related to Humicola insolens than to its own family. Similarity between the two was found to be 57.8%,whereas between Humicola lanuginosa (Thermomysis lanuginosus) and Humicola grisea it was 57.3%. Apart from this,similarity between Talaromyces dupontii and Rhizomucor pusillus was 51.5%. Least similarity was found in Rhizomucorpusillus and Humicola grisea, which was 18.7% and Chaetomium thermophile and Sporotrichum thermophile, which was18.3%. Genetic similarity matrix was constructed on the basis of Nei and Li’s index.http://rdo.psu.ac.th/sjstweb/journal/34-6/0597-0721-34-6-607-613.pdfgenetic diversitycellulolytic fungiDNA fingerprintingRAPD |
| spellingShingle | Asma Siddique Aftab Bashir Farooq Latif Exploration of unique relation among industrial fungi by statistical analysis Songklanakarin Journal of Science and Technology (SJST) genetic diversity cellulolytic fungi DNA fingerprinting RAPD |
| title | Exploration of unique relation among industrial fungi by statistical analysis |
| title_full | Exploration of unique relation among industrial fungi by statistical analysis |
| title_fullStr | Exploration of unique relation among industrial fungi by statistical analysis |
| title_full_unstemmed | Exploration of unique relation among industrial fungi by statistical analysis |
| title_short | Exploration of unique relation among industrial fungi by statistical analysis |
| title_sort | exploration of unique relation among industrial fungi by statistical analysis |
| topic | genetic diversity cellulolytic fungi DNA fingerprinting RAPD |
| url | http://rdo.psu.ac.th/sjstweb/journal/34-6/0597-0721-34-6-607-613.pdf |
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